Construct: ORF TRCN0000481334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010476.2_s317c1
- Derived from:
- ccsbBroadEn_03032
- DNA Barcode:
- AGTACACGATGTTCACGGGATGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HTATSF1 (27336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | NM_001163280.1 | 99.8% | 80.3% | 1816_1818delGGCinsCAG;1822delG |
| 2 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | NM_014500.5 | 99.8% | 80.3% | 1816_1818delGGCinsCAG;1822delG |
| 3 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | XM_005262404.4 | 99.8% | 80.3% | 1816_1818delGGCinsCAG;1822delG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1896
- ORF length:
- 1830
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcaccaac ttggatggga acgatgagtt tgatgagcag ttgcgaatgc 121 aagaattgta cggagacggc aaggatggtg acacccagac cgatgccggc ggagaacccg 181 attctctcgg gcagcagccg acggacactc cctacgagtg ggacctggac aaaaaggctt 241 ggttccccaa gattactgaa gatttcattg ctacatatca ggccaattat ggcttctcta 301 acgatggcgc atctagttct accgcaaatg ttgaagatgt ccatgctagg actgcagagg 361 aacctccaca agaaaaagcc ccggaaccca ctgatgccag aaagaaggga gaaaaaagaa 421 aggctgagtc aggatggttt catgttgaag aagacagaaa tacaaatgta tacgtgtctg 481 gtttgcctcc agatattaca gtggatgaat ttatacaact tatgtccaag tttggcatta 541 ttatgagaga tcctcagaca gaagaattta aggtcaaact ttacaaagat aatcaaggaa 601 atcttaaagg agacggtctt tgctgttatt tgaaaagaga atctgtggaa cttgcattaa 661 aacttttgga tgaagatgaa attagaggct acaaattaca tgttgaggtg gcaaagtttc 721 aactgaaggg agaatatgat gcctcaaaga agaagaagaa gtgcaaagac tataagaaga 781 agctgtctat gcaacaaaag cagttggatt ggagacctga gaggcgagcc ggaccatccc 841 ggatgcgcca tgagcgagtt gtcatcatca agaatatgtt tcatcctatg gattttgagg 901 atgatccgtt ggtgctgaat gagatcagag aagaccttcg agtagagtgt tcgaagtttg 961 gacaaattag gaaactcctt ctctttgata ggcacccaga tggtgtggcc tctgtgtcct 1021 ttcgggatcc agaggaagct gattattgta ttcagactct cgatggaaga tggtttggtg 1081 gccgtcaaat cactgcccag gcatgggatg ggactacaga ttatcaggtg gaggaaacct 1141 caagagaaag ggaggaaagg ctgagaggat gggaggcttt cctcaatgct cctgaggcca 1201 acagaggcct taggcgttca gattctgtct ctgcttccga aagggcaggg ccttctagag 1261 caaggcattt ttcagagcac cccagcacat ctaaaatgaa tgctcaagaa actgcaactg 1321 gaatggcgtt tgaagaacct atagatgaga agaagtttga aaagacagaa gatgggggag 1381 aatttgaaga aggtgcttct gaaaacaatg ctaaggaaag tagccccgaa aaagaggctg 1441 aagaaggctg ccctgaaaaa gaatctgaag agggctgccc caaaagaggg tttgaaggca 1501 gctgctccca aaaagagtct gaagaaggca atcccgtaag aggatctgaa gaggatagtc 1561 ctaaaaaaga gtctaaaaag aagacactca aaaatgattg tgaagagaat ggccttgcaa 1621 aggaatctga agatgacctc aacaaggagt ctgaagagga ggttggcccc acaaaagagt 1681 ccgaagaaga tgactcagag aaagagtctg atgaagactg ctctgaaaaa cagtctgaag 1741 atggctccga aagagaattt gaagaaaatg gtctcgagaa agatttggac gaggaaggtt 1801 ctgaaaagga gcttcatgaa aatgttcttg acaaagagtt agaagaaaat gactctgaaa 1861 actccgaatt tgaagatgaC CAGTCTAAAA AGTGTTAGAT GAGGAAGGCT CTGAGAGAGA 1921 GTTTGACGAA GATTCAGATG AAAAGGAAGA AGAGGAGGAT ACATATGAAA AAGTATTTGA 1981 TGATGAGTCT GATGAGAAAG AGGATGAAGA ATATGCAGAT GAAAAGGGGC TTGAAGCTGC 2041 TGATAAAAAG GCGGAAGAAG GTGATGCAGA TGAAAAGCTG TTTGAAGAGT CAGATGACAA 2101 GGAAGATGAA GATGCAGATG GAAAGGAAGT TGAAGATGCT GACGAAAAGT TGTTCGAAGA 2161 TGATGATTCC AATGAGAAGT TGTTTGATGA GGAGGAAGAT TCCAGTGAGA AGTTGTTTGA 2221 CGATTCTGAT GAGAGGGGGA CTTTGGGTGG TTTTGGGAGT GTTGAAGAAG GGCCCCTATC 2281 CACTGGCAGC AGCTTTATTC TCAGTAGCGA TGATGATGAC GATGATATTT ACCCAACTTT 2341 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 2401 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2461 TTGTGGAAAG GACGAAGTAC ACGATGTTCA CGGGATGTCA CGCGTTAAGT Cgacaatcaa 2521 cctctggatt acaaaatttg tgaaagatt