Construct: ORF TRCN0000481343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001588.1_s317c1
- Derived from:
- ccsbBroadEn_04611
- DNA Barcode:
- AGGTTCTTCACTCGAATTCATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTYH2 (94015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481343
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94015 | TTYH2 | tweety family member 2 | NM_032646.6 | 100% | 100% | |
| 2 | human | 94015 | TTYH2 | tweety family member 2 | NM_001330453.2 | 93.8% | 91.6% | (many diffs) |
| 3 | human | 94015 | TTYH2 | tweety family member 2 | NM_052869.1 | 39.8% | 39.8% | 0_1ins963 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1671
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcacaat gcaggcggcg cgcgtggact acatcgctcc ctggtgggtc gtgtggctgc 121 acagcgtccc gcacgtcggc ctgcgcctgc agcccgtgaa cagcaccttc agccccggcg 181 acgagagtta ccaggagtcg ctgctgttcc tggggctggt ggccgccgtc tgcctgggcc 241 tgaacctcat cttccttgtg gcttacctgg tctgtgcatg ccactgccgg cgggacgatg 301 cggtgcagac caagcagcac cactcctgct gcatcacctg gacggccgtg gtggccgggc 361 tcatctgctg tgctgcggtg ggcgttggtt tctatggaaa cagcgagacc aacgatgggg 421 cgtaccagct gatgtactcc ttggacgatg ccaaccacac cttctctggg atcgatgctc 481 tggtttccgg aactacccag aagatgaagg tggacctaga gcagcacctg gcccggctca 541 gtgagatctt tgctgcccgg ggcgattacc tgcagaccct gaagttcata cagcagatgg 601 cgggcagcgt tgttgttcag ctctcaggac tgcccgtgtg gagggaggtc accatggagc 661 tgaccaagct atccgaccag actggctacg tggagtacta caggtggctc tcctacctcc 721 tgctctttat cctggacctg gtcatctgcc tcattgcctg cctgggactg gccaagcgct 781 ccaagtgtct cctggcctcg atgctgtgct gtggggcact gagcctgctc ctcagttggg 841 catccctggc cgctgatggc tctgcggcag tggccaccag tgacttctgt gtggctcctg 901 acaccttcat cctgaacgtc acggagggcc agatcagcac agaggtgact cgctactacc 961 tgtattgcag ccagagtgga agcagcccct tccagcagac cctgaccacc ttccagcgcg 1021 cacttaccac catgcagatc caggtcgcgg ggctgctgca gtttgccgtg cccctcttct 1081 ccactgcaga ggaagacctg cttgcaatcc agctcctgct gaactcctca gagtccagcc 1141 ttcaccagct gaccgccatg gtggactgcc gagggctgca caaggattat ctggacgctc 1201 ttgctggcat ctgctacgac ggcctccagg gcttgctgta ccttggcctc ttctccttcc 1261 tggccgccct cgccttctcc accatgatct gtgcagggcc aagggCCTGG AAGCACTTCA 1321 CCACCAGAAA CAGAGACTAC GATGACATTG ATGATGATGA CCCCTTTAAC CCCCAAGCCT 1381 GGCGCATGGC GGCTCACAGT CCCCCGAGGG GACAGCTTCA CAGCTTCTGC AGCTACAGCA 1441 GTGGCCTGGG AAGTCAGACC AGCCTGCAGC CCCCGGCCCA GACCATCTCC AACGCCCCTG 1501 TCTCCGAGTA CATGAACCAA GCCATGCTCT TTGGTAGGAA CCCACGCTAC GAGAACGTGC 1561 CACTAATCGG GAGAGCCTCC CCTCCGCCTA CGTACTCTCC CAGCATGAGA GCCACCTACC 1621 TGTCTGTGGC GGATGAGCAC CTGAGGCACT ACGGGAATCA GTTTCCAGCC TTGCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGAAGGT TCTTCACTCG AATTCATATA ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt