Transcript: Human NM_052869.1

Homo sapiens tweety family member 2 (TTYH2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
TTYH2 (94015)
Length:
3278
CDS:
805..1446

Additional Resources:

NCBI RefSeq record:
NM_052869.1
NBCI Gene record:
TTYH2 (94015)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_052869.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219958 AGACACTTTGAAACGACTTTA pLKO.1 2229 3UTR 100% 13.200 18.480 N TTYH2 n/a
2 TRCN0000172434 CGAGTACATGAACCAAGCCAT pLKO.1 1278 CDS 100% 2.640 2.112 N TTYH2 n/a
3 TRCN0000219957 TACGAGAACGTGCCACTAATC pLKO.1 1321 CDS 100% 10.800 7.560 N TTYH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_052869.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04611 pDONR223 100% 39.8% 39.8% None 0_1ins963 n/a
2 ccsbBroad304_04611 pLX_304 0% 39.8% 39.8% V5 0_1ins963 n/a
3 TRCN0000481343 AGGTTCTTCACTCGAATTCATATA pLX_317 23.1% 39.8% 39.8% V5 0_1ins963 n/a
4 ccsbBroadEn_09364 pDONR223 100% 39.7% 39.7% None (many diffs) n/a
5 ccsbBroad304_09364 pLX_304 0% 39.7% 39.7% V5 (many diffs) n/a
6 TRCN0000481514 CTCAGCTTATGAAGAAGGTCCCCG pLX_317 23.6% 39.7% 39.7% V5 (many diffs) n/a
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