Construct: ORF TRCN0000481353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013725.1_s317c1
- Derived from:
- ccsbBroadEn_12472
- DNA Barcode:
- CACAAACGCGCGGACCTATCTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUDS3 (64426)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XM_017019820.1 | 72.8% | 72.8% | 1_189del |
| 2 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XM_017019818.1 | 65.7% | 65.7% | 1_264del |
| 3 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XM_017019819.1 | 65.7% | 65.7% | 1_264del |
| 4 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XM_017019817.1 | 55.2% | 55.2% | 1_411del |
| 5 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | NM_022491.3 | 51.5% | 51.5% | 1_477del |
| 6 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XM_011538671.2 | 41.5% | 41.5% | 1_714del |
| 7 | human | 64426 | SUDS3 | SDS3 homolog, SIN3A corepre... | XR_944677.2 | 5.9% | 1_3980del;4176_4177ins22;4466_8063del | |
| 8 | mouse | 71954 | Suds3 | suppressor of defective sil... | NM_178622.5 | 45.7% | 51.2% | (many diffs) |
| 9 | mouse | 71954 | Suds3 | suppressor of defective sil... | NM_001122666.2 | 44.8% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattgaaaat gaaaagctga caatggaact gactggagat tctatggagg 121 tgaaacctat catgaCCAGA AAGTTGCGGA GGCGACCAAA TGATCCCGTC CCCATCCCAG 181 ACAAGAGGAG GAAACCTGCT CCAGCCCAGC TAAACTATTT GTTAACAGAT GAACAGATCA 241 TGGAGGATCT GAGAACATTA AATAAGCTTA AGTCACCCAA GAGACCAGCA TCTCCATCCT 301 CTCCTGAGCA CTTGCCTGCA ACACCCGCGG AATCTCCAGC CCAGAGGTTC GAAGCTCGGA 361 TAGAAGATGG CAAACTGTAC TATGACAAAA GATGGTACCA CAAGAGCCAG GCCATCTATC 421 TGGAGTCAAA GGACAACCAG AAACTGAGCT GCGTGATCAG TTCTGTAGGA GCCAATGAGA 481 TCTGGGTGAG GAAGACAAGT GACAGCACCA AGATGAGGAT CTACCTGGGC CAGCTTCAGC 541 GCGGGCTCTT CGTGATCCGC CGGCGCTCAG CTGCTTTGCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ACACAAACGC GCGGACCTAT CTCGAACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt