Construct: ORF TRCN0000481367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004286.3_s317c1
- Derived from:
- ccsbBroadEn_01282
- DNA Barcode:
- ACAAAATTCATGAGACGGCGACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCB (5579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5579 | PRKCB | protein kinase C beta | NM_002738.7 | 100% | 100% | |
| 2 | human | 5579 | PRKCB | protein kinase C beta | NM_212535.3 | 95.3% | 95.6% | (many diffs) |
| 3 | mouse | 18751 | Prkcb | protein kinase C, beta | NM_008855.2 | 91.4% | 98.8% | (many diffs) |
| 4 | mouse | 18751 | Prkcb | protein kinase C, beta | NM_001316672.1 | 87.6% | 94.5% | (many diffs) |
| 5 | mouse | 18751 | Prkcb | protein kinase C, beta | XM_011241695.2 | 73.6% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2085
- ORF length:
- 2019
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaataag 61 ttggcatggc tgacccggct gcggggccgc cgccgagcga gggcgaggag agcaccgtgc 121 gcttcgcccg caaaggcgcc ctccggcaga agaacgtgca tgaggtcaag aaccacaaat 181 tcaccgcccg cttcttcaag cagcccacct tctgcagcca ctgcaccgac ttcatctggg 241 gcttcgggaa gcagggattc cagtgccaag tttgctgctt tgtggtgcac aagcggtgcc 301 atgaatttgt cacattctcc tgccctggcg ctgacaaggg tccagcctcc gatgaccccc 361 gcagcaaaca caagtttaag atccacacgt actccagccc cacgttttgt gaccactgtg 421 ggtcactgct gtatggactc atccaccagg ggatgaaatg tgacacctgc atgatgaatg 481 tgcacaagcg ctgcgtgatg aatgttccca gcctgtgtgg cacggaccac acggagcgcc 541 gcggccgcat ctacatccag gcccacatcg acagggacgt cctcattgtc ctcgtaagag 601 atgctaaaaa ccttgtacct atggacccca atggcctgtc agatccctac gtaaaactga 661 aactgattcc cgatcccaaa agtgagagca aacagaagac caaaaccatc aaatgctccc 721 tcaaccctga gtggaatgag acatttagat ttcagctgaa agaatcggac aaagacagaa 781 gactgtcagt agagatttgg gattgggatt tgaccagcag gaatgacttc atgggatctt 841 tgtcctttgg gatttctgaa cttcagaaag ccagtgttga tggctggttt aagttactga 901 gccaggagga aggcgagtac ttcaatgtgc ctgtgccacc agaaggaagt gaggccaatg 961 aagaactgcg gcagaaattt gagagggcca agatcagtca gggaaccaag gtcccggaag 1021 aaaagacgac caacactgtc tccaaatttg acaacaatgg caacagagac cggatgaaac 1081 tgaccgattt taacttccta atggtgctgg ggaaaggcag ctttggcaag gtcatgcttt 1141 cagaacgaaa aggcacagat gagctctatg ctgtgaagat cctgaagaag gacgttgtga 1201 tccaagatga tgacgtggag tgcactatgg tggagaagcg ggtgttggcc ctgcctggga 1261 agccgccctt cctgacccag ctccactcct gcttccagac catggaccgc ctgtactttg 1321 tgatggagta cgtgaatggg ggcgacctca tgtatcacat ccagcaagtc ggccggttca 1381 aggagcccca tgctgtattt tacgctgcag aaattgccat cggtctgttc ttcttacaga 1441 gtaagggcat catttaccgt gacctaaaac ttgacaacgt gatgctcgat tctgagggac 1501 acatcaagat tgccgatttt ggcatgtgta aggaaaacat ctgggatggg gtgacaacca 1561 agacattctg tggcactcca gactacatcg cccccgagat aattgcttat cagccctatg 1621 ggaagtccgt ggattggtgg gcatttggag tcctgctgta tgaaatgttG GCTGGGCAGG 1681 CACCCTTTGA AGGGGAGGAT GAAGATGAAC TCTTCCAATC CATCATGGAA CACAACGTAG 1741 CCTATCCCAA GTCTATGTCC AAGGAAGCTG TGGCCATCTG CAAAGGGCTG ATGACCAAAC 1801 ACCCAGGCAA ACGTCTGGGT TGTGGACCTG AAGGCGAACG TGATATCAAA GAGCATGCAT 1861 TTTTCCGGTA TATTGATTGG GAGAAACTTG AACGCAAAGA GATCCAGCCC CCTTATAAGC 1921 CAAAAGCTTG TGGGCGAAAT GCTGAAAACT TCGACCGATT TTTCACCCGC CATCCACCAG 1981 TCCTAACACC TCCCGACCAG GAAGTCATCA GGAATATTGA CCAATCAGAA TTCGAAGGAT 2041 TTTCCTTTGT TAACTCTGAA TTTTTAAAAC CCGAAGTCAA GAGCTACCCA ACTTTCTTGT 2101 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2161 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2221 GAAAGGACGA ACAAAATTCA TGAGACGGCG ACCGACGCGT TAAGTCgaca atcaacctct 2281 ggattacaaa atttgtgaaa gatt