Construct: ORF TRCN0000481372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000134.1_s317c1
- Derived from:
- ccsbBroadEn_03402
- DNA Barcode:
- ATCAGAACCTAGGAGGGTTACGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM11A (54065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102723553 | SMIM11B | small integral membrane pro... | NM_001313692.1 | 100% | 100% | |
| 2 | human | 54065 | SMIM11A | small integral membrane pro... | NM_058182.4 | 100% | 100% | |
| 3 | human | 102723553 | SMIM11B | small integral membrane pro... | XM_017028204.1 | 100% | 100% | |
| 4 | human | 102723553 | SMIM11B | small integral membrane pro... | XM_017028205.1 | 100% | 100% | |
| 5 | human | 102723553 | SMIM11B | small integral membrane pro... | XR_002958589.1 | 14.3% | 1_154del;329_1209del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 488
- ORF length:
- 174
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGAATT GGAAGGTTCT TGAGCACGTG CCCCTGCTGC TGTATATCTT 361 GGCAGCAAAA ACATTAATTC TCTGCCTGAC ATTTGCTGGG GTGAAAATGT ATCAAAGAAA 421 AAGGTTGGAG GCAAAACAAC AAAAACTGGA GGCTGAAAGG AAGAAGCAAT CAGAGAAAAA 481 AGATAACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 541 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 601 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATCAGAA CCTAGGAGGG TTACGTCACG 661 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt