Construct: ORF TRCN0000481391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000776.1_s317c1
- Derived from:
- ccsbBroadEn_04966
- DNA Barcode:
- CAGCCTCTCCAGACCCCCTGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPINDOC (144097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144097 | SPINDOC | spindlin interactor and rep... | NM_138471.3 | 99.9% | 100% | 574C>A |
| 2 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_011544770.1 | 94.1% | 79.2% | (many diffs) |
| 3 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_011544772.1 | 89.6% | 84.5% | 574C>A;927_928ins38;1026_1027ins79 |
| 4 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_006718437.1 | 88.5% | 80.1% | 574C>A;934_991del;1122_1123ins79 |
| 5 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_005273782.3 | 86.5% | 82.8% | (many diffs) |
| 6 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_011544771.1 | 83.6% | 69.1% | (many diffs) |
| 7 | human | 144097 | SPINDOC | spindlin interactor and rep... | XM_005273783.3 | 81.9% | 81.6% | (many diffs) |
| 8 | human | 144097 | SPINDOC | spindlin interactor and rep... | XR_001747777.1 | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctaaaggcc gagggcgccg cactcgactg cttcgaggtg acgctgaaat 121 gcgaggaagg ggaggacgag gaggaggcca tggtggtggc cgtaattccg cggcccgagc 181 cgatgctcag agtgacccaa caggagaaga ccccaccgcc tagacccagc ccgctagagg 241 caggcagtga tggctgtgag gagccgaagc agcaggtgtc ttgggagcag gagttcctgg 301 tgggcagcag cccaggaggc agcgggcggg cactgtgcat ggtgtgtggc gctgagatcc 361 gggcaccctc ggccgacaca gctcgctcgc acatcttgga gcagcaccct cacaccttgg 421 acctgagccc ttctgagaag agcaatatcc tggaggcctg gagtgaaggg gtggccctct 481 tgcaagacgt gagagctgag cagccgtccc cacccaactc agactcgggc caggatgccc 541 acccagaccc agacgccaac ccagacgctg ccagaatgcc agccgaaatc gtcgttctcc 601 ttgactctga ggataaccca tccctcccta aaaggagcag gcccagggga ctccgccccc 661 tcgagcttcc tgctgtccct gccacagagc caggaaataa gaagccccgt ggtcagagat 721 ggaaggaacc cccaggggaa gagccagtca gaaagaaaag aggCAGACCT ATGACCAAAA 781 ACCTGGACCC TGACCCAGAG CCCCCATCGC CAGACTCGCC CACGGAGACT TTCGCAGCAC 841 CAGCCGAGGT CCGACACTTC ACTGACGGCA GCTTCCCCGC CGGCTTCGTC TTGCAGCTCT 901 TCTCCCACAC CCAGCTCAGG GGCCCAGACA GCAAGGACTC ACCCAAAGAC AGGGAAGTGG 961 CAGAAGGAGG CCTTCCCCGG GCGGAGAGCC CCTCTCCAGC TCCCCCTCCG GGGCTCCGCG 1021 GGACACTGGA TCTCCAGGTT ATCCGCGTGC GGATGGAGGA GCCCCCAGCG GTCAGCCTCC 1081 TGCAAGACTG GTCCAGGCAC CCCCAGGGCA CCAAGCGTGT GGGAGCAGGT GACACCTCAG 1141 ACTGGCCCAC AGTTCTGTCA GAATCCAGCA CCACTGTGGC AGGGAAGCCG GAAAAAGGGA 1201 ATGGAGTGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAGCCT CTCCAGACCC CCTGCCTTAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt