Construct: ORF TRCN0000481441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016316.1_s317c1
- Derived from:
- ccsbBroadEn_08180
- DNA Barcode:
- GGCGGATGTTTAGCACCTCACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0A4 (50617)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50617 | ATP6V0A4 | ATPase H+ transporting V0 s... | NM_020632.3 | 99.9% | 99.8% | 755C>G;1662C>T |
| 2 | human | 50617 | ATP6V0A4 | ATPase H+ transporting V0 s... | NM_130840.3 | 99.9% | 99.8% | 755C>G;1662C>T |
| 3 | human | 50617 | ATP6V0A4 | ATPase H+ transporting V0 s... | NM_130841.2 | 99.9% | 99.8% | 755C>G;1662C>T |
| 4 | human | 50617 | ATP6V0A4 | ATPase H+ transporting V0 s... | XM_005250393.1 | 99.9% | 99.8% | 755C>G;1662C>T |
| 5 | human | 50617 | ATP6V0A4 | ATPase H+ transporting V0 s... | XM_005250394.3 | 99.9% | 99.8% | 755C>G;1662C>T |
| 6 | mouse | 140494 | Atp6v0a4 | ATPase, H+ transporting, ly... | NM_080467.3 | 83.8% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2589
- ORF length:
- 2520
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgtctgtg tttcgaagcg aggagatgtg tttgtcacaa ctgtttctcc 121 aggtggaagc tgcatattgc tgtgtggctg agctcggaga gctcggattg gttcagttca 181 aagatttaaa tatgaatgtg aacagctttc aaaggaaatt tgtgaatgaa gtcagaaggt 241 gtgaatcact ggagagaatc ctccgttttc tggaagacga gatgcaaaat gagattgtag 301 ttcagttgct cgagaaaagc ccactgaccc cgctcccacg ggaaatgatt accctggaga 361 ctgttctaga aaaactggaa ggagagttac aggaagccaa ccagaaccag caggccttga 421 aacaaagctt cctagaactg acagaactga aatacctcct gaagaaaacc caagacttct 481 ttgagacgga aaccaattta gctgatgatt tctttactga ggacacttct ggcctcctgg 541 agttgaaagc agtgcctgca tatatgaccg gaaagttggg gttcatagcc ggtgtgatca 601 acagggagag gatggcttcc tttgagcggt tactgtggcg aatctgccga ggaaacgtgt 661 acttgaagtt cagtgagatg gacgcccctc tggaggatcc tgtgacgaaa gaagaaattc 721 agaagaacat attcatcata ttttaccaag gagagcagct caggcagaaa atcaagaaga 781 tctgtgatgg gtttcgagcc actgtctacc cttgcccaga gcgtgcggtg gagcgcagag 841 agatgttgga gagcgtcaat gtgaggctgg aagatttaat caccgtcata acacaaacag 901 agtctcaccg ccagcgcctg ctgcaggaag ccgctgccaa ctggcactcc tggctcatca 961 aggtgcagaa gatgaaagct gtctaccaca tcctgaacat gtgcaacatc gacgtcaccc 1021 agcagtgtgt catcgccgag atctggttcc cggtggcaga tgccacacgt atcaagaggg 1081 cactggagca aggcatggaa ctaagtggct cctccatggc ccccatcatg accacagtgc 1141 aatctaaaac agcccctccc acatttaaca ggaccaataa attcacagct ggcttccaga 1201 atattgttga tgcctatggt gtcggcagct accgggagat aaacccagcc ccctacacca 1261 tcatcacttt ccccttcctg ttcgctgtga tgtttggaga ctgtggtcat ggaaccgtga 1321 tgctcctggc tgcactttgg atgattctga atgagagacg cttgctctcc cagaagacag 1381 acaatgagat ttggaacacc ttcttccacg ggcgctatct gatcctactt atgggcatct 1441 tctccatcta cacgggtttg atctacaatg actgcttctc caagtccttg aacatctttg 1501 gctcttcttg gagtgtccaa cccatgttca gaaacggcac atggaatact catgtaatgg 1561 aggaaagtct atatctgcag ctggacccag ccataccagg agtgtatttt ggaaatccat 1621 acccgtttgg gattgatccg atttggaact tggcttcaaa caaactcaca tttctgaact 1681 cgtataaaat gaagatgtcg gtgatcctgg gaattgtcca gatggttttt ggtgtcatcc 1741 tcagcctttt caatcacata tacttcagaa gaactctcaa catcattctg caatttatcc 1801 ctgagatgat ttttatcctg tgtctgtttg gatacctggt tttcatgatc attttcaaat 1861 ggtgctgctt tgacgtccat gtatctcagc acgcccccag catcctcatc cacttcatca 1921 acatgtttct gtttaactac agtgactctt ccaacgcacc cctctacaaa catcagcaag 1981 aagtccaaag tttctttgtg gttatggctt tgatttctgt gccgtggatg cttctgatta 2041 agccgtttat tcttagagcc agtcatcgga aatcccagct gcaggcatcc aggatccaag 2101 aagatgccac tgagaaCATT GAAGGTGATA GCTCCAGCCC TTCTAGCCGT TCTGGCCAGA 2161 GGACTTCTGC AGATACCCAC GGGGCTCTGG ACGACCATGG AGAAGAGTTC AACTTTGGAG 2221 ACGTCTTTGT CCACCAAGCC ATCCACACCA TCGAGTACTG CCTGGGCTGC ATTTCAAACA 2281 CAGCCTCCTA CCTGCGGCTC TGGGCCCTCA GCCTGGCTCA TGCACAACTG TCTGAAGTGC 2341 TCTGGACTAT GGTGATGAAC AGCGGCCTTC AGACGCGAGG CTGGGGAGGA ATCGTCGGGG 2401 TTTTTATTAT TTTTGCCGTA TTTGCTGTCC TGACAGTAGC CATCCTTCTG ATCATGGAGG 2461 GCCTCTCTGC TTTCCTGCAC GCCCTGCGAC TGCACTGGGT TGAGTTCCAG AACAAGTTCT 2521 ATGTCGGGGA TGGTTACAAG TTTTCTCCAT TCTCCTTTAA ACACATCCTG GATGGCACAG 2581 CCGAGGAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2641 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2701 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGCGGA TGTTTAGCAC CTCACCTTAC 2761 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt