Construct: ORF TRCN0000481444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013821.1_s317c1
- Derived from:
- ccsbBroadEn_05807
- DNA Barcode:
- TTGTCTGAGCACATACCTAATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALPL (249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_000478.6 | 99.8% | 99.8% | 330T>C;787T>C;876A>G |
| 2 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_001369803.2 | 99.8% | 99.8% | 330T>C;787T>C;876A>G |
| 3 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_001369804.2 | 99.8% | 99.8% | 330T>C;787T>C;876A>G |
| 4 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_001369805.2 | 99.8% | 99.8% | 330T>C;787T>C;876A>G |
| 5 | human | 249 | ALPL | alkaline phosphatase, biomi... | XM_017000903.1 | 89.8% | 85.6% | (many diffs) |
| 6 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_001127501.4 | 89.3% | 89.3% | (many diffs) |
| 7 | human | 249 | ALPL | alkaline phosphatase, biomi... | NM_001177520.3 | 85.1% | 79.1% | (many diffs) |
| 8 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | NM_001287172.1 | 84.6% | 90% | (many diffs) |
| 9 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | NM_007431.3 | 84.6% | 90% | (many diffs) |
| 10 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | XM_006538497.3 | 84.6% | 90% | (many diffs) |
| 11 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | XM_006538498.3 | 84.6% | 90% | (many diffs) |
| 12 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | XM_006538499.3 | 84.6% | 90% | (many diffs) |
| 13 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | XM_006538500.2 | 84.6% | 90% | (many diffs) |
| 14 | mouse | 11647 | Alpl | alkaline phosphatase, liver... | XM_017319924.1 | 84.6% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1641
- ORF length:
- 1572
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatttcacca ttcttagtac tggccattgg cacctgcctt actaactcct 121 tagtgccaga gaaagagaaa gaccccaagt actggcgaga ccaagcgcaa gagacactga 181 aatatgccct ggagcttcag aagctcaaca ccaacgtggc taagaatgtc atcatgttcc 241 tgggagatgg gatgggtgtc tccacagtga cggctgcccg catcctcaag ggtcagctcc 301 accacaaccc tggggaggag accaggctgg agatggacaa gttccccttc gtggccctct 361 ccaagacgta caacaccaat gcccaggtcc ctgacagcgc cggcaccgcc accgcctacc 421 tgtgtggggt gaaggccaat gagggcaccg tgggggtaag cgcagccact gagcgttccc 481 ggtgcaacac cacccagggg aacgaggtca cctccatcct gcgctgggcc aaggacgctg 541 ggaaatctgt gggcattgtg accaccacga gagtgaacca tgccaccccc agcgccgcct 601 acgcccactc ggctgaccgg gactggtact cagacaacga gatgccccct gaggccttga 661 gccagggctg taaggacatc gcctaccagc tcatgcataa catcagggac attgacgtga 721 tcatgggggg tggccggaaa tacatgtacc ccaagaataa aactgatgtg gagtatgaga 781 gtgacgagaa agccaggggc acgaggctgg acggcctgga cctcgttgac acctggaaga 841 gcttcaaacc gagacacaag cactcccact tcatctggaa ccgcacggaa ctcctgaccc 901 ttgaccccca caatgtggac tacctattgg gtctcttcga gccgggggac atgcagtacg 961 agctgaacag gaacaacgtg acggacccgt cactctccga gatggtggtg gtggccatcc 1021 agatcctgcg gaagaacccc aaaggcttct tcttgctggt ggaaggaggc agaattgacc 1081 acgggcacca tgaaggaaaa gccaagcagg ccctgcatga ggcggtggag atggaccggg 1141 ccatcgggca ggcaggcagc ttgacctcct cggaagacac tctgaccgtg gtcactgcgg 1201 accatTCCCA CGTCTTCACA TTTGGTGGAT ACACCCCCCG TGGCAACTCT ATCTTTGGTC 1261 TGGCCCCCAT GCTGAGTGAC ACAGACAAGA AGCCCTTCAC TGCCATCCTG TATGGCAATG 1321 GGCCTGGCTA CAAGGTGGTG GGCGGTGAAC GAGAGAATGT CTCCATGGTG GACTATGCTC 1381 ACAACAACTA CCAGGCGCAG TCTGCTGTGC CCCTGCGCCA CGAGACCCAC GGCGGGGAGG 1441 ACGTGGCCGT CTTCTCCAAG GGCCCCATGG CGCACCTGCT GCACGGCGTC CACGAGCAGA 1501 ACTACGTCCC CCACGTGATG GCGTATGCAG CCTGCATCGG GGCCAACCTC GGCCACTGTG 1561 CTCCTGCCAG CTCGGCAGGC AGCCTTGCTG CAGGCCCCCT GCTGCTCGCG CTGGCCCTCT 1621 ACCCCCTGAG CGTCCTGTTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTGT CTGAGCACAT 1801 ACCTAATACC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt