Transcript: Human NM_001127501.4

Homo sapiens alkaline phosphatase, biomineralization associated (ALPL), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
ALPL (249)
Length:
2371
CDS:
200..1609

Additional Resources:

NCBI RefSeq record:
NM_001127501.4
NBCI Gene record:
ALPL (249)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001127501.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359333 CATTCTCAAAGCCTCTTATTT pLKO_005 1887 3UTR 100% 15.000 10.500 N ALPL n/a
2 TRCN0000052005 GCGCAAGAGACACTGAAATAT pLKO.1 131 5UTR 100% 15.000 10.500 N ALPL n/a
3 TRCN0000359331 TTTGGCCAACAGGGTAGATTT pLKO_005 1834 3UTR 100% 13.200 9.240 N ALPL n/a
4 TRCN0000359332 AGTATGAGAGTGACGAGAAAG pLKO_005 738 CDS 100% 10.800 7.560 N ALPL n/a
5 TRCN0000359258 ATGTCTCCATGGTGGACTATG pLKO_005 1323 CDS 100% 10.800 7.560 N ALPL n/a
6 TRCN0000052003 CCCACAATGTGGACTACCTAT pLKO.1 873 CDS 100% 4.950 3.465 N ALPL n/a
7 TRCN0000052004 CGTGGCTAAGAATGTCATCAT pLKO.1 181 5UTR 100% 4.950 3.465 N ALPL n/a
8 TRCN0000052006 GAAGAGCTTCAAACCGAGATA pLKO.1 802 CDS 100% 4.950 3.465 N ALPL n/a
9 TRCN0000052007 ACTGCCATCCTGTATGGCAAT pLKO.1 1265 CDS 100% 0.405 0.284 N ALPL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127501.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05807 pDONR223 100% 89.3% 89.3% None (many diffs) n/a
2 ccsbBroad304_05807 pLX_304 0% 89.3% 89.3% V5 (many diffs) n/a
3 TRCN0000481444 TTGTCTGAGCACATACCTAATACC pLX_317 27.9% 89.3% 89.3% V5 (many diffs) n/a
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