Construct: ORF TRCN0000481468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010002.1_s317c1
- Derived from:
- ccsbBroadEn_03366
- DNA Barcode:
- CTACGTAAACCGGCCGTCGTGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS29 (51699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NM_057180.2 | 100% | 100% | |
| 2 | human | 51699 | VPS29 | VPS29 retromer complex comp... | XM_006719459.4 | 98% | 97.3% | 3_4insGCTGGGCAC;5_6delAGinsGA |
| 3 | human | 51699 | VPS29 | VPS29 retromer complex comp... | XM_006719460.4 | 98% | 97.3% | 3_4insGCTGGGCAC;5_6delAGinsGA |
| 4 | human | 51699 | VPS29 | VPS29 retromer complex comp... | XM_011538484.2 | 98% | 97.3% | 3_4insGCTGGGCAC;5_6delAGinsGA |
| 5 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NM_016226.5 | 97.8% | 97.3% | 3_4insGCTGGGCACAGA |
| 6 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NM_001282150.1 | 86.7% | 86.4% | 1_84del;86G>T |
| 7 | human | 51699 | VPS29 | VPS29 retromer complex comp... | XM_017019457.2 | 86.7% | 86.4% | 1_84del;86G>T |
| 8 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NM_001282151.1 | 48.9% | 36.7% | 3_4insGCTGGGCACAGA;195_196ins236;273_274ins37 |
| 9 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NR_104099.1 | 46.7% | 1_89del;105_144del;688_1193del | |
| 10 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NR_104100.1 | 46.2% | (many diffs) | |
| 11 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NR_104101.1 | 45.3% | 1_89del;105_182del;726_1231del | |
| 12 | human | 51699 | VPS29 | VPS29 retromer complex comp... | XM_017019459.2 | 44.2% | 33.7% | (many diffs) |
| 13 | human | 51699 | VPS29 | VPS29 retromer complex comp... | NR_104102.1 | 42.6% | (many diffs) | |
| 14 | mouse | 56433 | Vps29 | VPS29 retromer complex comp... | NM_001347453.1 | 90.1% | 99.4% | (many diffs) |
| 15 | mouse | 56433 | Vps29 | VPS29 retromer complex comp... | NM_019780.1 | 87.9% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 624
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggcacaga ttggtgttgg tattaggaga tctgcacatc ccacaccggt 121 gcaacagttt gccagctaaa ttcaaaaaac tcctggtgcc aggaaaaatt cagcaCATTC 181 TCTGCACAGG AAACCTTTGC ACCAAAGAGA GTTATGACTA TCTCAAGACT CTGGCTGGTG 241 ATGTTCATAT TGTGAGAGGA GACTTCGATG AGAATCTGAA TTATCCAGAA CAGAAAGTTG 301 TGACTGTTGG ACAGTTCAAA ATTGGTCTGA TCCATGGACA TCAAGTTATT CCATGGGGAG 361 ATATGGCCAG CTTAGCCCTG TTGCAGAGGC AATTTGATGT GGACATTCTT ATCTCGGGAC 421 ACACACACAA ATTTGAAGCA TTTGAGCATG AAAATAAATT CTACATTAAT CCAGGTTCTG 481 CCACTGGGGC ATATAATGCC TTGGAAACAA ACATTATTCC ATCATTTGTG TTGATGGATA 541 TCCAGGCTTC TACAGTGGTC ACCTATGTGT ATCAGCTAAT TGGAGATGAT GTGAAAGTAG 601 AACGAATCGA ATACAAAAAA CCTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TACGTAAACC 781 GGCCGTCGTG ACCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att