Construct: ORF TRCN0000481474

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003163.1_s317c1
Derived from:
ccsbBroadEn_03577
DNA Barcode:
CGTTTGATATCTAATTATGCCGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM144 (55314)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481474
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55314 TMEM144 transmembrane protein 144 NM_018342.5 100% 100%
2 human 55314 TMEM144 transmembrane protein 144 XM_005263110.2 100% 100%
3 human 55314 TMEM144 transmembrane protein 144 XM_005263112.3 100% 100%
4 human 55314 TMEM144 transmembrane protein 144 XM_006714254.1 100% 100%
5 human 55314 TMEM144 transmembrane protein 144 XM_017008366.1 100% 100%
6 human 55314 TMEM144 transmembrane protein 144 XM_024454128.1 100% 100%
7 human 55314 TMEM144 transmembrane protein 144 XM_017008367.1 55% 55% 0_1ins465
8 human 55314 TMEM144 transmembrane protein 144 XM_017008368.1 55% 55% 0_1ins465
9 mouse 70652 Tmem144 transmembrane protein 144 NM_027495.4 82.1% 85.9% (many diffs)
10 mouse 70652 Tmem144 transmembrane protein 144 XM_006502057.3 82.1% 85.9% (many diffs)
11 mouse 70652 Tmem144 transmembrane protein 144 XM_006502058.3 82.1% 85.9% (many diffs)
12 mouse 70652 Tmem144 transmembrane protein 144 XM_006502059.3 82.1% 85.9% (many diffs)
13 mouse 70652 Tmem144 transmembrane protein 144 XM_011240224.2 82.1% 85.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1101
ORF length:
1035
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag caacaatgga gcagacctaa cctttggtta catctcctgt tttgtagcta
121tccttttgtt tggctcaaat tttgtgccac ttaaaaaatt tgatactggt gatggaatgt
181ttctccagtg ggttctttgt gctgccatat ggttggttgc cttggttgtc aatctgatat
241tacattgtcc aaagttttgg ccttttgcaa tgcttggggg ctgcatttgg gcaacaggga
301acattgctgt tgtcccaatt atcaaaacca ttggtttagg ccttggaatc ttaatctggg
361gatcatttaa tgccttaact ggctgggcaa gctcaaggtt tggctggttt ggattggatg
421cagaagaagt atcaaatccg ctgctaaatt acattggagc tgggctatca gtagtaagtg
481ctttcatatt tttgttcatc aaaagtgaaa taccaaataa cacgtgttcc atggatacca
541ctccattaat aacagagcat gtgatcaaca caacccaaga cccctgttcc tgggtggata
601aactttctac agtacaccac cgcatagtgg gctgcagtct tgcagtgata TCTGGAGTAC
661TCTATGGATC TACATTTGTG CCAATCATCT ACATCAAGGA CCACAGCAAA AGAAATGATA
721GTATATATGC AGGGGCAAGC CAATATGATT TAGACTATGT GTTTGCGCAC TTCAGTGGCA
781TCTTTCTTAC AAGTACTGTC TACTTTCTGG CCTACTGCAT AGCCATGAAA AATAGTCCTA
841AACTATATCC TGAAGCAGTC CTACCAGGAT TCCTGTCAGG AGTACTTTGG GCTATAGCTA
901CCTGCTGTTG GTTCATAGCA AATCACTCTC TGAGTGCTGT GGTCAGTTTT CCAATAATCA
961CTGCTGGTCC AGGATTTATA GCTGCAATGT GGGGTATCTT CATGTTTAAG GAAATAAAGG
1021GTCTACAAAA CTACCTATTA ATGATACTTG CATTTTGCAT CATCTTGACT GGAGCCTTAT
1081GCACTGCTTT TTCTAAAATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1141CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1201AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGTT TGATATCTAA
1261TTATGCCGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)