Construct: ORF TRCN0000481485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012537.1_s317c1
- Derived from:
- ccsbBroadEn_07944
- DNA Barcode:
- TCGCATAATTGTACTGTGCAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRI3 (25798)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25798 | BRI3 | brain protein I3 | NM_015379.5 | 99.7% | 99.2% | 157C>T |
| 2 | human | 25798 | BRI3 | brain protein I3 | XM_017011936.1 | 75.5% | 57.7% | (many diffs) |
| 3 | human | 25798 | BRI3 | brain protein I3 | NM_001159491.2 | 74.9% | 62.4% | (many diffs) |
| 4 | human | 25798 | BRI3 | brain protein I3 | XM_017011935.2 | 66.3% | 34.3% | (many diffs) |
| 5 | human | 25798 | BRI3 | brain protein I3 | XM_017011932.2 | 65.5% | 31.5% | (many diffs) |
| 6 | human | 25798 | BRI3 | brain protein I3 | XM_017011933.2 | 65.5% | 31.5% | (many diffs) |
| 7 | human | 25798 | BRI3 | brain protein I3 | XM_017011934.2 | 65.5% | 31.5% | (many diffs) |
| 8 | human | 25798 | BRI3 | brain protein I3 | XM_017011931.2 | 63.2% | 30.5% | (many diffs) |
| 9 | mouse | 55950 | Bri3 | brain protein I3 | NM_018772.4 | 89.3% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 80
- ORF end:
- 455
- ORF length:
- 375
- Sequence:
-
1 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 61 TTTGTACAAA AAAGTTGGCA TGGACCACAA GCCGCTGCTG CAGGAGCGGC CGCCCGCCTA 121 CAACCTGGAG GCCGGCCAGG GCGACTACGC GTGCGGCCCG CACGGCTACG GCGCCATCCC 181 CGCCGCGCCC CCGCCGCCGC CCTACCCCTA CCTCGTCACA GGGATACCCA CCCACTATCC 241 CAGGGTCTAC AACATCCACA GCCGGACCGT CACCCGCTAT CCTGCCAACT CTATCGTGGT 301 CGTAGGAGGC TGTCCTGTCT GCAGGGTTGG GGTGCTGGAG GACTGCTTCA CCTTCCTGGG 361 CATCTTCCTG GCCATCATCC TCTTCCCCTT TGGGTTCATT TGCTGTTTTG CCTTGAGGAA 421 GCGACGATGC CCCAACTGTG GAGCCACCTT CGCTTACCCA ACTTTCTTGT ACAAAGTGGT 481 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 541 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 601 TCGCATAATT GTACTGTGCA GGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa 661 atttgtgaaa gatt