Construct: ORF TRCN0000481502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016904.1_s317c1
- Derived from:
- ccsbBroadEn_15267
- DNA Barcode:
- GGTCAACCAACAGGACACAGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R1C (151242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001080545.2 | 100% | 100% | |
| 2 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001261425.2 | 100% | 100% | |
| 3 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001261424.2 | 93.9% | 93.9% | 79_99del |
| 4 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | XM_017003451.1 | 70.5% | 56.8% | (many diffs) |
| 5 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NR_048567.1 | 24.2% | (many diffs) | |
| 6 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NR_048566.2 | 10.3% | 1_258del;586_3173del | |
| 7 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_172420.4 | 87.4% | 84.4% | (many diffs) |
| 8 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_001290743.1 | 74.4% | 68.8% | (many diffs) |
| 9 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_001290744.1 | 61.7% | 60.5% | (many diffs) |
| 10 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NR_110980.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 396
- ORF length:
- 327
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGAGCCCAAC AGTCCCAAAA AGATACAGTT TGCCGTGCCT GTATTCCAGA 121 GTCAGATTGC ACCTGAAGCA GCAGAGCAGA TCAGGAAAAG AAGACCTACA CCAGCATCAC 181 TTGTGATTCT CAATGAGCAT AACCCCCCAG AAATAGATGA CAAGAGGGGG CCCAACACAC 241 AAGGGGAATT ACAGAATGCA TCCCCTAAGC AAAGGAAGCA GAGTGTGTAC ACACCACCCA 301 CCATAAAAGG GGTTAAGCAT CTGAAAGGCC AGAATGAATC AGCATTCCCT GAAGAAGAAG 361 AAGGCACCAA TGAAAGAGAG GAGCAGCGGG ACCATTTGCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AGGTCAACCA ACAGGACACA GACGCACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt