Construct: ORF TRCN0000481508

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010225.1_s317c1
Derived from:
ccsbBroadEn_07931
DNA Barcode:
TCCGAAAGAGCCTGGATGACGTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FLRT1 (23769)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481508
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23769 FLRT1 fibronectin leucine rich tr... NM_013280.4 99.9% 100% 714C>T
2 human 23769 FLRT1 fibronectin leucine rich tr... XM_005273861.2 99.9% 100% 714C>T;1893A>G
3 human 23769 FLRT1 fibronectin leucine rich tr... XM_011544883.2 99.9% 100% 714C>T;1893A>G
4 human 23769 FLRT1 fibronectin leucine rich tr... XM_024448413.1 99.9% 100% 714C>T;1893A>G
5 mouse 396184 Flrt1 fibronectin leucine rich tr... NM_201411.2 89.2% 96.1% (many diffs)
6 mouse 396184 Flrt1 fibronectin leucine rich tr... XM_006527169.2 89.2% 96.1% (many diffs)
7 mouse 396184 Flrt1 fibronectin leucine rich tr... XM_006527170.3 89.2% 96.1% (many diffs)
8 mouse 396184 Flrt1 fibronectin leucine rich tr... XM_006527171.3 89.2% 96.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2088
ORF length:
2022
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ggtggcacac cccaccgcca ctgccaccac cacgcccact gccactgtca
121cggccaccgt tgtgatgacc acggccacca tggacctgcg ggactggctg ttcctctgct
181acgggctcat cgccttcctg acggaggtca tcgacagcac cacctgcccc tcggtgtgcc
241gctgcgacaa cggcttcatc tactgcaacg accggggact cacatccatc cccgcagata
301tccctgatga tgccaccacc ctctacctgc agaacaacca gatcaacaac gccggcatcc
361cccaggacct caagaccaag gtcaacgtgc aggtcatcta cctatacgag aatgacctgg
421atgagttccc catcaacctg ccccgctccc tccgggagct gcacctgcag gacaacaatg
481tgcgcaccat tgccagggac tcgctggccc gcatcccgct gctggagaag ctgcacctgg
541atgacaactc cgtgtccacc gtcagcattg aggaggacgc cttcgccgac agcaaacagc
601tcaagctgct cttcctgagc cggaaccacc tgagcagcat cccctcgggg ctgccgcaca
661cgctggagga gctgcggctg gatgacaacc gcatctccac catcccgctg catgccttca
721agggcctcaa cagcctgcgg cgcctggtgc tggacggtaa cctgctggcc aaccagcgta
781tcgccgacga caccttcagc cgcctacaga acctcacaga gctctcgctg gtgcgcaatt
841cgctggccgc gccacccctc aacctgccca gcgcccacct gcagaagctc tacctgcagg
901acaatgccat cagccacatc ccctacaaca cgctggccaa gatgcgtgag ctggagcggc
961tggacctgtc caacaacaac ctgaccacgc tgccccgcgg cctgttcgac gacctgggga
1021acctggccca gctgctgctc aggaacaacc cttggttttg tggctgcaac ctcatgtggc
1081tgcgggactg ggtgaaggca cgggcggccg tggtcaacgt gcggggcctc atgtgccagg
1141gccctgagaa ggtccggggc atggccatca aggacattac cagcgagatg gacgagtgtt
1201ttgagacggg gccgcagggc ggcgtggcca atgcggctgc caagaccacg gccagcaacc
1261acgcctctgc caccacgccc cagggttccc tgtttaccct caaggccaaa aggccagggc
1321tgcgcctccc cgactccaac attgactacc ccatggccac gggtgatggc gccaagaccc
1381tggccatcca cgtgaaggcc ctgacggcag actccatccg catcacgtgg aaggccacgc
1441tccccgcctc ctctttccgg ctcagttggc tgcgcctggg ccacagccca gccgtgggct
1501ccatcacgga gaccttggtg cagggggaca agacagagta cctgctgaca gccctggagc
1561ccaagtccac ctacatcatc tgcatggtca ccatggagac cagcaatgcc tacGTAGCTG
1621ATGAGACACC CGTGTGTGCC AAGGCAGAGA CAGCCGACAG CTATGGCCCT ACCACCACAC
1681TCAACCAGGA GCAGAACGCT GGCCCCATGG CGAGCCTGCC CCTGGCGGGC ATCATCGGCG
1741GGGCAGTGGC TCTGGTCTTC CTCTTCCTGG TCCTGGGGGC CATCTGCTGG TACGTGCACC
1801AGGCTGGCGA GCTGCTGACC CGGGAGAGGG CCTACAACCG GGGCAGCAGG AAAAAGGATG
1861ACTATATGGA GTCAGGGACC AAGAAGGATA ACTCCATCCT GGAAATCCGC GGCCCTGGGC
1921TGCAGATGCT GCCCATCAAC CCGTACCGCG CCAAAGAGGA GTACGTGGTC CACACTATCT
1981TCCCCTCCAA CGGCAGCAGC CTCTGCAAGG CCACACACAC CATTGGCTAC GGCACCACGC
2041GGGGCTACCG GGACGGCGGC ATCCCCGACA TAGACTACTC CTACACATGC CCAACTTTCT
2101TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2161CGTAGTAATG AACTAGTCCG CAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2221GTGGAAAGGA CGATCCGAAA GAGCCTGGAT GACGTTGACG CGTTAAGTCg acaatcaacc
2281tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)