Construct: ORF TRCN0000481508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010225.1_s317c1
- Derived from:
- ccsbBroadEn_07931
- DNA Barcode:
- TCCGAAAGAGCCTGGATGACGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLRT1 (23769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481508
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23769 | FLRT1 | fibronectin leucine rich tr... | NM_013280.4 | 99.9% | 100% | 714C>T |
| 2 | human | 23769 | FLRT1 | fibronectin leucine rich tr... | XM_005273861.2 | 99.9% | 100% | 714C>T;1893A>G |
| 3 | human | 23769 | FLRT1 | fibronectin leucine rich tr... | XM_011544883.2 | 99.9% | 100% | 714C>T;1893A>G |
| 4 | human | 23769 | FLRT1 | fibronectin leucine rich tr... | XM_024448413.1 | 99.9% | 100% | 714C>T;1893A>G |
| 5 | mouse | 396184 | Flrt1 | fibronectin leucine rich tr... | NM_201411.2 | 89.2% | 96.1% | (many diffs) |
| 6 | mouse | 396184 | Flrt1 | fibronectin leucine rich tr... | XM_006527169.2 | 89.2% | 96.1% | (many diffs) |
| 7 | mouse | 396184 | Flrt1 | fibronectin leucine rich tr... | XM_006527170.3 | 89.2% | 96.1% | (many diffs) |
| 8 | mouse | 396184 | Flrt1 | fibronectin leucine rich tr... | XM_006527171.3 | 89.2% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2088
- ORF length:
- 2022
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtggcacac cccaccgcca ctgccaccac cacgcccact gccactgtca 121 cggccaccgt tgtgatgacc acggccacca tggacctgcg ggactggctg ttcctctgct 181 acgggctcat cgccttcctg acggaggtca tcgacagcac cacctgcccc tcggtgtgcc 241 gctgcgacaa cggcttcatc tactgcaacg accggggact cacatccatc cccgcagata 301 tccctgatga tgccaccacc ctctacctgc agaacaacca gatcaacaac gccggcatcc 361 cccaggacct caagaccaag gtcaacgtgc aggtcatcta cctatacgag aatgacctgg 421 atgagttccc catcaacctg ccccgctccc tccgggagct gcacctgcag gacaacaatg 481 tgcgcaccat tgccagggac tcgctggccc gcatcccgct gctggagaag ctgcacctgg 541 atgacaactc cgtgtccacc gtcagcattg aggaggacgc cttcgccgac agcaaacagc 601 tcaagctgct cttcctgagc cggaaccacc tgagcagcat cccctcgggg ctgccgcaca 661 cgctggagga gctgcggctg gatgacaacc gcatctccac catcccgctg catgccttca 721 agggcctcaa cagcctgcgg cgcctggtgc tggacggtaa cctgctggcc aaccagcgta 781 tcgccgacga caccttcagc cgcctacaga acctcacaga gctctcgctg gtgcgcaatt 841 cgctggccgc gccacccctc aacctgccca gcgcccacct gcagaagctc tacctgcagg 901 acaatgccat cagccacatc ccctacaaca cgctggccaa gatgcgtgag ctggagcggc 961 tggacctgtc caacaacaac ctgaccacgc tgccccgcgg cctgttcgac gacctgggga 1021 acctggccca gctgctgctc aggaacaacc cttggttttg tggctgcaac ctcatgtggc 1081 tgcgggactg ggtgaaggca cgggcggccg tggtcaacgt gcggggcctc atgtgccagg 1141 gccctgagaa ggtccggggc atggccatca aggacattac cagcgagatg gacgagtgtt 1201 ttgagacggg gccgcagggc ggcgtggcca atgcggctgc caagaccacg gccagcaacc 1261 acgcctctgc caccacgccc cagggttccc tgtttaccct caaggccaaa aggccagggc 1321 tgcgcctccc cgactccaac attgactacc ccatggccac gggtgatggc gccaagaccc 1381 tggccatcca cgtgaaggcc ctgacggcag actccatccg catcacgtgg aaggccacgc 1441 tccccgcctc ctctttccgg ctcagttggc tgcgcctggg ccacagccca gccgtgggct 1501 ccatcacgga gaccttggtg cagggggaca agacagagta cctgctgaca gccctggagc 1561 ccaagtccac ctacatcatc tgcatggtca ccatggagac cagcaatgcc tacGTAGCTG 1621 ATGAGACACC CGTGTGTGCC AAGGCAGAGA CAGCCGACAG CTATGGCCCT ACCACCACAC 1681 TCAACCAGGA GCAGAACGCT GGCCCCATGG CGAGCCTGCC CCTGGCGGGC ATCATCGGCG 1741 GGGCAGTGGC TCTGGTCTTC CTCTTCCTGG TCCTGGGGGC CATCTGCTGG TACGTGCACC 1801 AGGCTGGCGA GCTGCTGACC CGGGAGAGGG CCTACAACCG GGGCAGCAGG AAAAAGGATG 1861 ACTATATGGA GTCAGGGACC AAGAAGGATA ACTCCATCCT GGAAATCCGC GGCCCTGGGC 1921 TGCAGATGCT GCCCATCAAC CCGTACCGCG CCAAAGAGGA GTACGTGGTC CACACTATCT 1981 TCCCCTCCAA CGGCAGCAGC CTCTGCAAGG CCACACACAC CATTGGCTAC GGCACCACGC 2041 GGGGCTACCG GGACGGCGGC ATCCCCGACA TAGACTACTC CTACACATGC CCAACTTTCT 2101 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2161 CGTAGTAATG AACTAGTCCG CAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2221 GTGGAAAGGA CGATCCGAAA GAGCCTGGAT GACGTTGACG CGTTAAGTCg acaatcaacc 2281 tctggattac aaaatttgtg aaagatt