Construct: ORF TRCN0000481510
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009685.1_s317c1
- Derived from:
- ccsbBroadEn_06630
- DNA Barcode:
- GTCCAGATGAACATCACGTCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEFM (4741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481510
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4741 | NEFM | neurofilament medium | NM_005382.2 | 99.9% | 99.8% | 1315C>A |
| 2 | human | 4741 | NEFM | neurofilament medium | NM_001105541.2 | 58.9% | 58.8% | 0_1ins1128;187C>A |
| 3 | mouse | 18040 | Nefm | neurofilament, medium polyp... | NM_008691.2 | 81.2% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2817
- ORF length:
- 2748
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctacacg ttggactcgc tgggcaaccc gtccgcctac cggcgggtaa 121 ccgagacccg ctcgagcttc agccgcgtca gcggctcccc gtccagtggc ttccgctcgc 181 agtcgtggtc ccgcggctcg cccagcaccg tgtcctcctc ctataagcgc agcatgctcg 241 ccccgcgcct cgcttacagc tcggccatgc tcagctccgc cgagagcagc cttgacttca 301 gccagtcctc gtccctgctc aacggcggct ccggacccgg cggcgactac aagctgtccc 361 gctccaacga gaaggagcag ctgcaggggc tgaacgaccg ctttgccggc tacatagaga 421 aggtgcacta cctggagcag cagaataagg agattgaggc ggagatccag gcgctgcggc 481 agaagcaggc ctcgcacgcc cagctgggcg acgcgtacga ccaggagatc cgcgagctgc 541 gcgccaccct ggagatggtg aaccacgaga aggctcaggt gcagctggac tcggaccacc 601 tggaggaaga catccaccgg ctcaaggagc gctttgagga ggaggcgcgg ttgcgcgacg 661 acactgaggc ggccatccgc gcgctgcgca aagacatcga ggaggcgtcg ctggtcaagg 721 tggagctgga caagaaggtg cagtcgctgc aggatgaggt ggccttcctg cggagcaacc 781 acgaggagga ggtggccgac cttctggccc agatccaggc atcgcacatc acggtggagc 841 gcaaagacta cctgaagaca gacatctcga cggcgctgaa ggaaatccgc tcccagctcg 901 aaagccactc agaccagaat atgcaccagg ccgaagagtg gttcaaatgc cgctacgcca 961 agctcaccga ggcggccgag cagaacaagg aggccatccg ctccgccaag gaagagatcg 1021 ccgagtaccg gcgccagctg cagtccaaga gcatcgagct agagtcggtg cgcggcacca 1081 aggagtccct ggagcggcag ctcagcgaca tcgaggagcg ccacaaccac gacctcagca 1141 gctaccagga caccatccag cagctggaaa atgagcttcg gggcacaaag tgggaaatgg 1201 ctcgtcattt gcgcgaatac caggacctcc tcaacgtcaa gatggctctg gatatagaaa 1261 tcgctgcgta cagaaaactc ctggagggtg aagagactag atttagcaca tttgcaggaa 1321 gcatcactgg gccactgtat acacaccgac ccccaatcac aatatccagt aagattcaga 1381 aaaccaaggt ggaagctccc aagcttaagg tccaacacaa atttgtcgag gagatcatag 1441 aggaaaccaa agtggaggat gagaagtcag aaatggaaga ggccctgaca gccattacag 1501 aggaattggc cgtttccatg aaggaagaga agaaagaagc agcagaagaa aaggaagagg 1561 aacccgaagc tgaagaagaa gaagtagctg ccaaaaagtc tccagtgaaa gcaactgcac 1621 ctgaagttaa agaagaggaa ggggaaaagg aggaagaaga aggccaggaa gaagaggagg 1681 aagaagatga gggagctaag tcagaccaag ccgaagaggg aggatccgag aaggaaggct 1741 ctagtgaaaa agaggaaggt gagcaggaag aaggagaaac agaagctgaa gctgaaggag 1801 aggaagccga agctaaagag gaaaagaaag tggaggaaaa gagtgaggaa gtggctacca 1861 aggaggagct ggtggcagat gccaaggtgg aaaagccaga aaaagccaag tctcctgtgc 1921 caaaatcacc agtggaagag aaaggcaagt ctcctgtgcc caagtcacca gtggaagaga 1981 aaggcaagtc tcctgtgccc aagtcaccag tggaagagaa aggcaagtct cctgtgccga 2041 aatcaccagt ggaagagaaa ggcaagtctc ctgtgtcaaa atcaccagtg gaagagaaag 2101 ccaaatctcc tgtgccaaaa tcaccagtgg aagaggcaaa gtcaaaagca gaagtgggga 2161 aaggtgaaca gaaagaggaa gaagaaaagg aagtcaagga agctcccaag gaagagaagg 2221 tagagaaaaa ggaagagaaa ccaaaggatg tgccagagaa gaagaaagct gagtcccctg 2281 taaaggagga agctgtggca gaggtggtca ccatcaccaa atcggtaaag gtgcacttGG 2341 AGAAAGAGAC CAAAGAAGAG GGGAAGCCAC TGCAGCAGGA GAAAGAGAAG GAGAAAGCGG 2401 GAGGAGAGGG AGGAAGTGAG GAGGAAGGGA GTGATAAAGG TGCCAAGGGA TCCAGGAAGG 2461 AAGACATAGC TGTCAATGGG GAGGTAGAAG GAAAAGAGGA GGTAGAGCAG GAGACCAAGG 2521 AAAAAGGCAG TGGGAGGGAA GAGGAGAAAG GCGTTGTCAC CAATGGCCTA GACTTGAGCC 2581 CAGCAGATGA AAAGAAGGGG GGTGATAAAA GTGAGGAGAA AGTGGTGGTG ACCAAAACGG 2641 TAGAAAAAAT CACCAGTGAG GGGGGAGATG GTGCTACCAA ATACATCACT AAATCTGTAA 2701 CCGTCACTCA AAAGGTTGAA GAGCATGAAG AGACCTTTGA GGAGAAACTA GTGTCTACTA 2761 AAAAGGTAGA AAAAGTCACT TCACACGCCA TAGTAAAGGA AGTCACCCAG AGTGACTTGC 2821 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2881 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2941 ATATATCTTG TGGAAAGGAC GAGTCCAGAT GAACATCACG TCCCCTACGC GTTAAGTCga 3001 caatcaacct ctggattaca aaatttgtga aagatt