Construct: ORF TRCN0000481518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014600.1_s317c1
- Derived from:
- ccsbBroadEn_06774
- DNA Barcode:
- AATCGCTGTAAGTTTAGGCCGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCQ (5588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323265.1 | 99.9% | 99.8% | 27G>N |
| 2 | human | 5588 | PRKCQ | protein kinase C theta | NM_006257.5 | 99.9% | 99.8% | 27G>N |
| 3 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448076.1 | 99.9% | 99.8% | 27G>N |
| 4 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252496.4 | 95.3% | 95.2% | 1_102del;129G>N |
| 5 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282644.2 | 94.9% | 94.3% | 0_1ins106;10_11insAG |
| 6 | human | 5588 | PRKCQ | protein kinase C theta | NM_001242413.2 | 91% | 90.9% | 27G>N;1645_1646ins189 |
| 7 | human | 5588 | PRKCQ | protein kinase C theta | XM_005252497.4 | 89.1% | 88.5% | (many diffs) |
| 8 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323267.1 | 88.4% | 87.2% | (many diffs) |
| 9 | human | 5588 | PRKCQ | protein kinase C theta | NM_001282645.1 | 82.2% | 82.1% | 0_1ins372;3_4insAGT |
| 10 | human | 5588 | PRKCQ | protein kinase C theta | NM_001323266.2 | 82.2% | 82.1% | 0_1ins372;3_4insAGT |
| 11 | human | 5588 | PRKCQ | protein kinase C theta | XM_024448077.1 | 82.2% | 82.1% | 0_1ins372;3_4insAGT |
| 12 | mouse | 18761 | Prkcq | protein kinase C, theta | NM_008859.2 | 87.1% | 94.4% | (many diffs) |
| 13 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497398.3 | 81.9% | 88.8% | (many diffs) |
| 14 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_011238965.2 | 72.6% | 77.2% | (many diffs) |
| 15 | mouse | 18761 | Prkcq | protein kinase C, theta | XM_006497399.2 | 55.9% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2187
- ORF length:
- 2118
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgccattt cttcggattg gcttntccaa ctttgactgc gggtcctgcc 121 agtcttgtca gggcgaggct gttaaccctt actgtgctgt gctcgtcaaa gagtatgtcg 181 aatcagagaa cgggcagatg tatatccaga aaaagcctac catgtaccca ccctgggaca 241 gcacttttga tgcccatatc aacaagggaa gagtcatgca gatcattgtg aaaggcaaaa 301 acgtggacct catctctgaa accaccgtgg agctctactc gctggctgag aggtgcagga 361 agaacaacgg gaagacagaa atatggttag agctgaaacc tcaaggccga atgctaatga 421 atgcaagata ctttctggaa atgagtgaca caaaggacat gaatgaattt gagacggaag 481 gcttctttgc tttgcatcag cgccggggtg ccatcaagca ggcaaaggtc caccacgtca 541 agtgccacga gttcactgcc accttcttcc cacagcccac attttgctct gtctgccacg 601 agtttgtctg gggcctgaac aaacagggct accagtgccg acaatgcaat gcagcaattc 661 acaagaagtg tattgataaa gttatagcaa agtgcacagg atcagctatc aatagccgag 721 aaaccatgtt ccacaaggag agattcaaaa ttgacatgcc acacagattt aaagtctaca 781 attacaagag cccgaccttc tgtgaacact gtgggaccct gctgtgggga ctggcacggc 841 aaggactcaa gtgtgatgca tgtggcatga atgtgcatca tagatgccag acaaaggtgg 901 ccaacctttg tggcataaac cagaagctaa tggctgaagc gctggccatg attgagagca 961 ctcaacaggc tcgctgctta agagatactg aacagatctt cagagaaggt ccggttgaaa 1021 ttggtctccc atgctccatc aaaaatgaag caaggccgcc atgtttaccg acaccgggaa 1081 aaagagagcc tcagggcatt tcctgggagt ctccgttgga tgaggtggat aaaatgtgcc 1141 atcttccaga acctgaactg aacaaagaaa gaccatctct gcagattaaa ctaaaaattg 1201 aggattttat cttgcacaaa atgttgggga aaggaagttt tggcaaggtc ttcctggcag 1261 aattcaagaa aaccaatcaa tttttcgcaa taaaggcctt aaagaaagat gtggtcttga 1321 tggacgatga tgttgagtgc acgatggtag agaagagagt tctttccttg gcctgggagc 1381 atccgtttct gacgcacatg ttttgtacat tccagaccaa ggaaaacctc ttttttgtga 1441 tggagtacct caacggaggg gacttaatgt accacatcca aagctgccac aagttcgacc 1501 tttccagagc gacgttttat gctgctgaaa tcattcttgg tctgcagttc cttcattcca 1561 aaggaatagt ctacagggac ctgaagctag ataacatcct gttagacaaa gatggacata 1621 tcaagatcgc ggattttgga atgtgcaagg agaacatgtt aggagatgcc aagacgaata 1681 ccttctgtgg gacacctgac tacatcgccc cagagatctt gctgggtcag aaatacaacc 1741 actctgtgga ctggtggtcc ttcggggttc tcctttatga aatgctgatt ggtcagtcgc 1801 ctttccacgg gcaggatgag gaggagctct tccactccat ccgcatggac aatcccttTT 1861 ACCCACGGTG GCTGGAGAAG GAAGCAAAGG ACCTTCTGGT GAAGCTCTTC GTGCGAGAAC 1921 CTGAGAAGAG GCTGGGCGTG AGGGGAGACA TCCGCCAGCA CCCTTTGTTT CGGGAGATCA 1981 ACTGGGAGGA ACTTGAACGG AAGGAGATTG ACCCACCGTT CCGGCCGAAA GTGAAATCAC 2041 CATTTGACTG CAGCAATTTC GACAAAGAAT TCTTAAACGA GAAGCCCCGG CTGTCATTTG 2101 CCGACAGAGC ACTGATCAAC AGCATGGACC AGAATATGTT CAGGAACTTT TCCTTCATGA 2161 ACCCCGGGAT GGAGCGGCTG ATATCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATCGCTG 2341 TAAGTTTAGG CCGCAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt