Construct: ORF TRCN0000481532

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017157.1_s317c1
Derived from:
ccsbBroadEn_09882
DNA Barcode:
AACAAGTTACTTTTCCAGCTACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC35F1 (222553)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481532
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 222553 SLC35F1 solute carrier family 35 me... NM_001029858.4 99.8% 99.5% 461C>A;902C>A
2 human 222553 SLC35F1 solute carrier family 35 me... XM_005266865.4 99.8% 99.5% 461C>A;902C>A
3 mouse 215085 Slc35f1 solute carrier family 35, m... NM_178675.4 90% 96.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1293
ORF length:
1224
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gatcccccct gagcagccgc agcagcagct gcagccgccg tcgccagccc
121cgccgaacca tgtggtgacc accatcgaga acctgccggc cgagggcagc ggcggcggcg
181ggagcctgtc cgcctcctcc cgggctggcg tgcgccagag gatccgcaaa gtgctgaaca
241gggagatgtt aatctctgtg gccctaggcc aggtgttatc cctccttatt tgtggaattg
301gcttgactag caagtatctg tcagaagatt tccacgccaa cacaccagtc ttccagagtt
361tcctcaatta cattcttctc ttcttggtct ataccaccac actagccgtc agacaaggag
421aagaaaacct cctggcaatt ttacgacgaa gatggtggaa gtacatgatt ttgggactca
481tagacctgga agcaaattat ctggtggtca aggcttacca atacacaaat ctgaccagta
541tccagctcct ggactgtttt gtgatcccag tcgtgatttt gctctcctgg ttcttcctgc
601tgatccggta caaggctgtg catttcatcg gcatcgttgt ctgcatcctg ggaatgggct
661gcatggtggg agcagatgtg cttgtgggaa gacatcaggg agcaggggaa aataagctgg
721taggggacct tctggtctta ggaggagcca cactctatgg tatttctaac gtctgggaag
781aatacatcat ccgaactcTG AGCCGAGTGG AATTCCTGGG AATGATTGGT CTCTTTGGAG
841CATTTTTCAG TGGAATTCAA TTAGCTATAA TGGAGCATAA GGAACTGTTG AAGGTGCCCT
901GGGACTGGCA AATAGGACTG CTCTACGTTG GCTTTAGTGC CTGCATGTTT GGTCTCTACA
961GCTTTATGCA AGTCGTCATA AAGAAAACCA GTGCCACTTC AGTCAACCTC TCCTTGCTCA
1021CAGCAGACTT GTACAGCCTG TTCTGTGGAT TGTTTCTCTT CCACTACAAG TTTTCAGGAC
1081TTTATCTCCT GTCTTTCTTC ACCATCCTCA TTGGGCTGGT GCTCTACTCC TCCACCTCCA
1141CCTACATAGC CCAGGACCCC CGAGTGTATA AGCAGTTCCG CAATCCTTCA GGACCTGTTG
1201TGGACTTACC GACCACAGCT CAGGTGGAAC CCTCAGTCAC CTACACCAGC CTGGGCCAGG
1261AGACCGAAGA GGAGCCTCAT GTTCGTGTGG CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG
1321ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1381GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA
1441CAAGTTACTT TTCCAGCTAC GTACGCGTTA AGTCgacaat caacctctgg attacaaaat
1501ttgtgaaaga tt

Download FASTA (ORF) (Full)