Construct: ORF TRCN0000481540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007225.1_s317c1
- Derived from:
- ccsbBroadEn_08988
- DNA Barcode:
- ATTGGGGCATGCCGCAGCAGTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM110D (79927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79927 | FAM110D | family with sequence simila... | NM_024869.3 | 99.7% | 99.6% | 237G>A;811G>C |
| 2 | mouse | 72690 | Grrp1 | glycine/arginine rich prote... | NM_001099296.2 | 86.7% | 86.7% | (many diffs) |
| 3 | mouse | 72690 | Grrp1 | glycine/arginine rich prote... | XM_006539198.2 | 86.7% | 86.7% | (many diffs) |
| 4 | mouse | 72690 | Grrp1 | glycine/arginine rich prote... | XM_006539199.3 | 86.7% | 86.7% | (many diffs) |
| 5 | mouse | 72690 | Grrp1 | glycine/arginine rich prote... | XM_006539200.2 | 86.7% | 86.7% | (many diffs) |
| 6 | mouse | 72690 | Grrp1 | glycine/arginine rich prote... | XM_006539197.2 | 77.6% | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cctggcccct ccctccaccc cgtccagagg acggaccccc agcgccgtgg 121 agaggctgga agccgacaaa gccaagtatg tcaagacgca ccaggtgata gctaggcgac 181 aggagccagc cctgcgtggg agtcctgggc cgctcacgcc gcacccctgc aacgagctgg 241 ggccccctgc atcgcccagg acgcccaggc cggtccgccg gggaagcggc aggcggctgc 301 caaggcctga ttccctcatc ttctaccgcc agaagcggga ctgcaaggct tcggtgaaca 361 aagagaacgc caagggccag ggtctggtgc ggcgcctctt tctgggtgcc ccgcgggacg 421 ctgccccgag cagcccggcc tccacagagc gacctGCGGC TTCAGGGGGT TGGGCTGCGC 481 CCCAGGATGC CCCGGAAGCG GCGGGAAAGC GGGCGCTGTG TCCCACGTGC TCGCTGCCCC 541 TGTCGGAGAA GGAGCGCTTC TTCAACTACT GCGGCCTGGA GCGCGCGCTG GTGGAGGTGC 601 TGGGCGCAGA GCGCTTCTCC CCGCAGAGCT GGGGAGCCGA CGCCAGCCCG CAGGCCGGAA 661 CTTCGCCGCC GCCCGGCTCC GGGGACGCCA GCGACTGGAC ATCCAGCGAC AGGGGCGTGG 721 ACAGCCCGGG CGGCGCGGGC GGCGGCGGCG GCTCGGAGGC AGCGGGCTCG GCGCGGGACC 781 GGCGCCCCCC GGTGTCGGTG GTGGAGCGCA ACGCGCGCGT CATCCAGTGG CTGTACGGCT 841 GCCAGCGCGC CCGCGGACCG CCGCGCGAGT CCGAGCTGTG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGAATTGGG GCATGCCGCA GCAGTCATAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt