Construct: ORF TRCN0000481543
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003966.1_s317c1
- Derived from:
- ccsbBroadEn_12577
- DNA Barcode:
- AGACAATGCAAGACCGGAAAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM184A (79632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481543
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79632 | FAM184A | family with sequence simila... | NM_024581.6 | 96.5% | 96.4% | 1_117del;130A>T;587G>A |
| 2 | human | 79632 | FAM184A | family with sequence simila... | NM_001100411.2 | 88.1% | 88.1% | 0_1ins243;227G>A;2408_2409ins147 |
| 3 | human | 79632 | FAM184A | family with sequence simila... | NM_001288576.1 | 84.9% | 84.9% | (many diffs) |
| 4 | mouse | 75906 | Fam184a | family with sequence simila... | NM_001081428.1 | 82.7% | 85.8% | (many diffs) |
| 5 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512889.3 | 81.7% | 84.7% | (many diffs) |
| 6 | mouse | 75906 | Fam184a | family with sequence simila... | XM_017314133.1 | 79.8% | 82.9% | (many diffs) |
| 7 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512890.3 | 79.6% | 82.5% | (many diffs) |
| 8 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512891.3 | 78.9% | 81.9% | (many diffs) |
| 9 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512894.3 | 78.6% | 81.5% | (many diffs) |
| 10 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512893.3 | 77.6% | 80.5% | (many diffs) |
| 11 | mouse | 75906 | Fam184a | family with sequence simila... | XM_011243225.2 | 75.7% | 78.6% | (many diffs) |
| 12 | mouse | 75906 | Fam184a | family with sequence simila... | XM_017314136.1 | 75.2% | 78.6% | (many diffs) |
| 13 | mouse | 75906 | Fam184a | family with sequence simila... | XM_017314134.1 | 74.8% | 77.7% | (many diffs) |
| 14 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512895.3 | 74.7% | 77.7% | (many diffs) |
| 15 | mouse | 75906 | Fam184a | family with sequence simila... | XM_017314135.1 | 74.2% | 77.7% | (many diffs) |
| 16 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512896.3 | 71.9% | 74.8% | (many diffs) |
| 17 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512897.3 | 71.4% | 74.8% | (many diffs) |
| 18 | mouse | 75906 | Fam184a | family with sequence simila... | XR_380088.3 | 67.7% | (many diffs) | |
| 19 | mouse | 75906 | Fam184a | family with sequence simila... | XM_006512898.3 | 56.9% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3369
- ORF length:
- 3303
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cctgaaattg agcaagaaaa tcgcccagct caccaaggta atatatgctt 121 taaacactaa aaatgatgag catgaatctg caattcaagc cctcaaagat gctcatgaag 181 aagaaattca acaaattctt gctgaaacaa gagaaaaaat attgcagtat aaaagcaaag 241 taacagagga gctagacctt agaagaaaga ttcaagtttt agaatcatca ttagaagatc 301 acataaaaat gaagcagcag gctttgacag aatttgaagc ttataagcac agagttgagg 361 acatgcaact ttgtgcagaa gcccagcatg tccaacgcat agtgaccatg tctagagaag 421 tcgaagagat tagaaggaaa tttgaagaaa aattacggag ctttggacaa cttcaagtac 481 agtttgaaaa agacaaacga ttggcattgg aagacttgca agctgctcac agacaggaga 541 tacaagagct attgaagtca cagcaggatc acagtgcctc agtaaataaa ggccaggaaa 601 aggcagagga actacacaga atggaggtgg agtccctaaa caaaatgctt gaggagctaa 661 gacttgaacg gaagaaacta attgaggatt atgaaggcaa gttgaataaa gctcagtcct 721 tttatgaacg tgagcttgat actttgaaaa ggtcacagct ttttacagca gaaagcctac 781 aggccagcaa agaaaaggaa gctgatctta gaaaagaatt tcagggacaa gaagcaattt 841 tacgaaaaac tataggaaaa ttaaagacag agttacagat ggtacaggat gaagctggaa 901 gtcttcttga caaatgccaa aagcttcaga cggcacttgc catagcagag aacaatgttc 961 aggttcttca aaaacagctt gatgatgcca aggagggaga aatggcccta ttaagcaagc 1021 acaaagaagt ggaaagtgag ctagcagctg ccagagaacg tttacaacag caagcttcag 1081 atcttgtcct caaagctagt catattggaa tgcttcaagc aactcaaatg acccaggaag 1141 ttacaattaa agatttagaa tcagaaaaat cgagagtcaa tgagagatta tctcaacttg 1201 aagaggaaag agcttttttg cgaagcaaaa cccaaagtct ggatgaagag cagaagcaac 1261 agattctaga actggagaag aaagtaaatg aagcaaagag aactcagcaa gaatattatg 1321 aaagggaact taaaaacctg caaagtagat tggaagagga ggtgactcaa ttaaacgagg 1381 cccattctaa gactttggaa gaattagctt ggaagcacca tatggcaatt gaagctgtcc 1441 acagtaatgc aattagggat aagaaaaaac tgcaaatgga tttggaagaa caacataaca 1501 aagataaact aaacctggaa gaggataaaa atcagcttca acaagagcta gaaaacctaa 1561 aggaagtact ggaagacaag ttgaatacag ccaatcaaga gattggccac ctccaagata 1621 tggtaaggaa aagtgaacaa ggtcttggct ctgcagaagg acttattgct agtcttcagg 1681 actcccagga aaggcttcag aatgagcttg acttgactaa agacagccta aaggagacca 1741 aggatgctct attaaatgtg gagggtgagc tagaacaaga aaggcaacag catgaagaaa 1801 caattgctgc catgaaagaa gaagagaagc tcaaagtgga caaaatggcc catgacttag 1861 aaattaagtg gactgaaaat cttagacaag agtgttctaa acttcgtgaa gagttaaggc 1921 ttcaacatga agaggataag aagtcagcaa tgtctcaact tttgcagttg aaagatcgag 1981 agaaaaatgc agcaagagat tcatggcaga agaaagtaga agatctctta aaccagattt 2041 ccttgctgaa acagaatctg gagatacagc tttcccagtc tcagacttct ttgcaacaac 2101 tgcaagccca gtttacgcaa gaacgacagc ggcttacgca agagcttgaa gaattagagg 2161 agcaacatca gcaaagacac aaatcattaa aagaagcaca tgtccttgca tttcaaacta 2221 tggaagagga aaaggaaaag gagcaaagag ctcttgaaaa tcatttacaa cagaagcatt 2281 ctgcagagct tcaatcacta aaagatgcac acagagagtc aatggagggc ttccggatag 2341 aaatggaaca ggaacttcag actcttcggt ttgaattaga agatgaagga aaggctatgc 2401 ttgcttcctt gcgctcagaa ctcaaccatc aacatgcagc tgcaattgat ttgttacggc 2461 ataatcatca tcaagaattg gcagctgcta aaatggaatt agagagaagc atagacatca 2521 gcagaagaca gagtaaggag cacatatgta gaattacaga tctacaagag gaattaagac 2581 acagagagca tcacatctct gaattggata aggaggttca gcaccttcat gagaatataa 2641 gtgccctaac caaagaactg gaatttaagg ggaaagaaat tctcagaata cgaagtgaat 2701 ctaaccaaca gataaggttg catgaacaag atttaaacaa gagacttgaa aaagagttgg 2761 atgtcatgac agcagaccac ctcagagaga aaaatatcat gcgggcagat tttaataaga 2821 ctaacgagct actcaaggaa ataaatgccg ctttacaagt gtcattagaa gaaatggaag 2881 aaaaatatct aatgagagaa tcaaaaccag aagatataca gatgattaca gaattaaaag 2941 ccatgcttac agaaagagac cagaTCATAA AGAAACTAAT TGAGGATAAT AAGTTTTATC 3001 AGCTGGAATT AGTCAATCGA GAAACTAACT TCAACAAAGT GTTTAACTCA AGTCCTACTG 3061 TTGGTGTTAT TAATCCATTG GCTAAGCAAA AGAAGAAGAA TGATAAATCA CCAACAAACA 3121 GGTTTGTGAG TGTTCCCAAT CTAAGTGCTC TGGAATCTGG TGGAGTGGGC AATGGACATC 3181 CTAACCGCCT GGATCCCATT CCTAATTCTC CAGTCCACGA TATTGAGTTC AACAGCAGCA 3241 AACCACTTCC ACAGCCAGTG CCACCTAAAG GGCCCAAGAC ATTTTTGAGT CCTGCTCAGA 3301 GTGAAGCTTC TCCAGTGGCT TCTCCAGATC CCCAGCGCCA GGAGTGGTTT GCCCGGTACT 3361 TCACATTCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGACAA TGCAAGACCG GAAAGCGGAC 3541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt