Construct: ORF TRCN0000481551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003255.1_s317c1
- Derived from:
- ccsbBroadEn_09737
- DNA Barcode:
- CCATCTGTTACCGTGTTGTCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRED1 (161742)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161742 | SPRED1 | sprouty related EVH1 domain... | NM_152594.3 | 99.8% | 100% | 291G>A;1044T>C |
| 2 | human | 161742 | SPRED1 | sprouty related EVH1 domain... | XM_005254202.3 | 97.2% | 97.1% | 32_67del;327G>A;1080T>C |
| 3 | human | 161742 | SPRED1 | sprouty related EVH1 domain... | XM_011521289.3 | 95.1% | 95.2% | 0_1ins63;228G>A;981T>C |
| 4 | mouse | 114715 | Spred1 | sprouty protein with EVH-1 ... | NM_033524.3 | 91% | 93% | (many diffs) |
| 5 | mouse | 114715 | Spred1 | sprouty protein with EVH-1 ... | NM_001277256.1 | 45.1% | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1401
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgaggag acggcgactt ctgacaacga taatagttat gcacgagtgc 121 gagctgtggt gatgacccga gatgactcaa gtggtggatg gttaccactt ggagggagtg 181 gactaagcag cgtcactgtc ttcaaagtcc ctcatcagga agagaatggc tgtgctgact 241 tttttatccg tggagagcga ctcagggaca aaatggtggt tttggaatgt atgcttaaaa 301 aagacctcat ttataataag gtcactccaa catttcacca ctggaagatt gatgacaaaa 361 agtttggtct tacgtttcaa agtcctgctg atgctagggc ttttgataga ggtatccgaa 421 gagctataga ggatatttct caaggatgcc ccgaatcaaa aaatgaagct gaaggggcag 481 atgacttaca agcaaatgaa gaggattctt ccagttctct agtgaaggat cacctttttc 541 agcaagagac agttgttacc agtgagcctt atagaagctc aaatataaga ccttctccct 601 ttgaagatct gaatgccaga agagtctaca tgcaaagcca agccaatcag ataacatttg 661 gtcagccagg cttggacatt cagagcagaa gtatggaata cgtacagcgg caaatatcca 721 aggaatgtgg aagcctaaag tcccaaaata gggtcccttt gaaatcaatc agacatgtca 781 gctttcaaga tgaggatgag attgtcagaa taaaccctcg agatatctta atacgtcgct 841 atgcagacta cagacatcct gacatgtgga aaaatgactt ggaaagagat gatgctgatt 901 ccagtattca gttttctaaa ccagacagta aaaaatcaga ctatctgtac tcttGTGGGG 961 ATGAGACTAA GTTAAGTTCA CCCAAAGACT CTGTGGTATT TAAGACGCAG CCTTCCTCAT 1021 TAAAAATTAA GAAGTCAAAA CGAAGAAAAG AGGATGGTGA ACGTTCTCGC TGCGTATACT 1081 GCCAGGAAAG GTTTAATCAT GAAGAAAATG TCAGGGGAAA ATGTCAGGAT GCTCCAGACC 1141 CTATTAAAAG ATGCATATAT CAAGTTAGTT GCATGCTCTG TGCAGAGAGC ATGTTGTATC 1201 ATTGTATGTC AGACTCAGAG GGAGATTTTT CTGATCCCTG TTCGTGTGAC ACTAGCGACG 1261 ACAAGTTCTG CTTGCGATGG TTAGCCCTGG TAGCTTTGTC TTTCATTGTA CCATGTATGT 1321 GCTGCTACGT CCCTTTGAGA ATGTGCCATC GCTGTGGTGA GGCATGTGGT TGCTGTGGTG 1381 GGAAACATAA AGCTGCTGGA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAT CTGTTACCGT 1561 GTTGTCGAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt