Construct: ORF TRCN0000481560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002945.1_s317c1
- Derived from:
- ccsbBroadEn_08356
- DNA Barcode:
- CTCTGCCCATAACTTCCAGGACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIL3 (53938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | NM_130906.3 | 99.7% | 99.3% | 438T>G |
| 2 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_005246652.5 | 99.7% | 99.3% | 438T>G |
| 3 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_011511358.2 | 99.7% | 99.3% | 438T>G |
| 4 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004355.2 | 99.7% | 99.3% | 438T>G |
| 5 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004356.2 | 99.7% | 99.3% | 438T>G |
| 6 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | NM_032472.4 | 88% | 74.8% | (many diffs) |
| 7 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_005246651.3 | 88% | 74.8% | (many diffs) |
| 8 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004352.1 | 88% | 74.8% | (many diffs) |
| 9 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004353.1 | 88% | 74.8% | (many diffs) |
| 10 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004354.2 | 88% | 74.8% | (many diffs) |
| 11 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004357.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 12 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004358.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 13 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004359.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 14 | human | 53938 | PPIL3 | peptidylprolyl isomerase li... | XM_017004360.1 | 69.3% | 68.9% | 0_1ins147;291T>G |
| 15 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_001285826.1 | 93.1% | 97.5% | (many diffs) |
| 16 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_001285827.1 | 93.1% | 97.5% | (many diffs) |
| 17 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_027351.3 | 93.1% | 97.5% | (many diffs) |
| 18 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238590.2 | 74.9% | 61.3% | (many diffs) |
| 19 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238591.2 | 74.9% | 61.3% | (many diffs) |
| 20 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238592.2 | 74.9% | 61.3% | (many diffs) |
| 21 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238593.2 | 74.9% | 61.3% | (many diffs) |
| 22 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238594.2 | 74.9% | 61.3% | (many diffs) |
| 23 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | XM_011238595.2 | 74.9% | 61.3% | (many diffs) |
| 24 | mouse | 70225 | Ppil3 | peptidylprolyl isomerase (c... | NM_027374.3 | 68.9% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 549
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgacactg catacagatg taggtgatat taaaattgaa gtcttctgtg 121 agaggacacc caaaacatgt gagaatttct tGGCTCTTTG TGCCAGTAAT TACTACAATG 181 GCTGTATATT TCATAGGAAT ATCAAGGGTT TCATGGTTCA AACAGGAGAT CCAACAGGAA 241 CTGGAAGAGG AGGCAACAGT ATTTGGGGCA AGAAGTTTGA GGATGAATAC AGTGAATATC 301 TTAAGCACAA TGTTAGAGGT GTTGTATCTA TGGCTAATAA TGGCCCGAAC ACCAATGGAT 361 CTCAGTTCTT CATCACCTAT GGCAAACAGC CACATTTGGA CATGAAATAC ACCGTATTTG 421 GAAAGGTAAT AGATGGTCTG GAAACTCTAG ATGAGTTGGA GAAGTTGCCA GTAAATGAGA 481 AGACATACCG ACCTCTTAAT GAGGTACACA TTAAGGACAT AACTATTCAT GCCAACCCAT 541 TTGCTCAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCTGC CCATAACTTC CAGGACTAAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt