Transcript: Mouse XM_011238594.2

PREDICTED: Mus musculus peptidylprolyl isomerase (cyclophilin)-like 3 (Ppil3), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ppil3 (70225)
Length:
863
CDS:
260..790

Additional Resources:

NCBI RefSeq record:
XM_011238594.2
NBCI Gene record:
Ppil3 (70225)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011238594.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000101282 CCACACTTGGACATGAAATAT pLKO.1 584 CDS 100% 15.000 10.500 N Ppil3 n/a
2 TRCN0000231438 GTTGTATCTATGGCTAATAAT pLKO_005 515 CDS 100% 15.000 10.500 N PPIL3 n/a
3 TRCN0000101283 GCCACACTTGGACATGAAATA pLKO.1 583 CDS 100% 13.200 9.240 N Ppil3 n/a
4 TRCN0000101281 CGGCTGTGTGTTTCATAGAAA pLKO.1 373 CDS 100% 5.625 3.938 N Ppil3 n/a
5 TRCN0000000186 AGGTGTTGTATCTATGGCTAA pLKO.1 511 CDS 100% 4.050 2.835 N PPIL3 n/a
6 TRCN0000000187 GAGGTGTTGTATCTATGGCTA pLKO.1 510 CDS 100% 2.640 1.848 N PPIL3 n/a
7 TRCN0000000185 TCTCAGTTCTTCATCACCTAT pLKO.1 554 CDS 100% 4.950 2.970 N PPIL3 n/a
8 TRCN0000107330 CCATGATGATTTGTTCCAGTT pLKO.1 652 CDS 100% 4.050 2.835 N ZNF282 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011238594.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08356 pDONR223 100% 74.9% 61.3% None (many diffs) n/a
2 ccsbBroad304_08356 pLX_304 0% 74.9% 61.3% V5 (many diffs) n/a
3 TRCN0000481560 CTCTGCCCATAACTTCCAGGACTA pLX_317 81.1% 74.9% 61.3% V5 (many diffs) n/a
4 TRCN0000488890 ACGCTGCTCGCCTAACGTAACAAC pLX_317 74.8% 74.9% 61.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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