Construct: ORF TRCN0000481569
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013504.1_s317c1
- Derived from:
- ccsbBroadEn_07826
- DNA Barcode:
- TATTTCAATAACAGGATGTTAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHDC10 (23008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481569
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23008 | KLHDC10 | kelch domain containing 10 | NM_014997.4 | 99.9% | 99.7% | 302A>G |
| 2 | human | 23008 | KLHDC10 | kelch domain containing 10 | XM_005250233.5 | 93.3% | 93.2% | 164_165ins87;215A>G |
| 3 | mouse | 76788 | Klhdc10 | kelch domain containing 10 | NM_029742.3 | 90.4% | 96.3% | (many diffs) |
| 4 | mouse | 76788 | Klhdc10 | kelch domain containing 10 | NM_001311087.1 | 84% | 89.8% | (many diffs) |
| 5 | mouse | 76788 | Klhdc10 | kelch domain containing 10 | XM_017321829.1 | 81.2% | 81.5% | (many diffs) |
| 6 | mouse | 76788 | Klhdc10 | kelch domain containing 10 | XM_030255666.1 | 59.2% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1392
- ORF length:
- 1326
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggccgcccag ggctgggaca ggaaccgccg gaggggagga ggcgccgccg 121 gcgctggtgg cggaggtagc ggggccggcg ggggcagtgg gggcagcggg ggtcggggga 181 ctggccagct caaccgcttc gtgcaactct ccgggcggcc gcacctgcca ggtaagaaga 241 aaatacgatg ggacccagtt aggaggcgct tcattcagtc ctgtcccatc ataaggatcc 301 ctaacaggtt tttgagaggc cacagacctc caccagcacg aagtggacat cgttgtgtgg 361 cagatagtac caacctatat gtgtttggag gttataaccc agattatgat gaatcgggag 421 ggcctgataa tgaagactat cctctcttca gggaactctg gaggtatcat tttgctacag 481 gagtatggca ccagatgggc acagatggct acatgccccg ggaattggca tctatgtcac 541 ttgtgctgca tggaaacaac ctgttagtat ttggaggtac gggcatccca tttggagaga 601 gcaacggcaa tgacgtccat gtgtgtaatg tgaagtataa gagatgggct ttgctcagct 661 gtcgggggaa gaaacccagt cgtatatatg gacaggctat ggccatcatc aatggctccc 721 tttatgtctt tggaggtaca accggctata tttacagcac agacctgcac aagttagatc 781 tcaataccag agagtggaca caactgaaac caaacaacct atcctgtgat ctaccagaag 841 agagataccg acatgaaatt gcacatgacg ggcagaggat ttacatcttg ggaggtggta 901 cttcctggac agcatattcc ttaaacaaga tccatgcata caaccttgaa acgaatgcct 961 GGGAGGAAAT TGCAACAAAA CCCCATGAAA AAATAGGCTT TCCTGCAGCC CGAAGGTGTC 1021 ACAGTTGTGT TCAAATAAAA AATGATGTAT TTATTTGTGG GGGCTATAAT GGAGAGGTGA 1081 TCCTGGGAGA TATCTGGAAG TTGAATCTGC AGACTTTCCA ATGGGTGAAG CTCCCAGCTA 1141 CCATGCCAGA GCCAGTTTAT TTTCACTGTG CAGCTGTTAC ACCAGCTGGT TGCATGTACA 1201 TTCATGGAGG AGTGGTGAAC ATCCATGAAA ACAAACGGAC TGGGTCATTG TTTAAGATCT 1261 GGCTGGTGGT ACCTAGCCTG CTGGAACTGG CATGGGAGAA GCTGCTTGCG GCCTTCCCTA 1321 ACCTTGCAAA CCTCTCCCGA ACACAACTTC TGCACCTTGG ACTCACACAG GGACTCATCG 1381 AACGCTTGAA ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAT TTCAATAACA GGATGTTAGG 1561 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t