Construct: ORF TRCN0000481574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008850.3_s317c1
- Derived from:
- ccsbBroadEn_06706
- DNA Barcode:
- ACTGCAGCTACAGCAGGAGCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRL (5157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481574
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_006207.2 | 99.9% | 100% | 1041T>C |
| 2 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_001372073.1 | 99.8% | 100% | 792T>C;1041T>C |
| 3 | mouse | 68797 | Pdgfrl | platelet-derived growth fac... | NM_026840.3 | 84.1% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggtctggctg ctgcttggtc ttctgctggt gcacgaagcg ctggaggatg 121 ttactggcca acaccttccc aagaacaagc gtccaaaaga accaggagag aatagaatca 181 aacctaccaa caagaaggtg aagcccaaaa ttcctaaaat gaaggacagg gactcagcca 241 attcagcacc aaagacgcag tctatcatga tgcaagtgct ggataaaggt cgcttccaga 301 aacccgccgc taccctgagt ctgctggcgg ggcaaactgt agagcttcga tgtaaaggga 361 gtagaattgg gtggagctac cctgcgtatc tggacacctt taaggattct cgcctcagcg 421 tcaagcagaa tgagcgctac ggccagttga ctctggtcaa ctccacctcg gcagacacag 481 gtgaattcag ctgctgggtg cagctctgca gcggctacat ctgcaggaag gacgaggcca 541 aaacgggctc cacctacatc ttttttacag agaaaggaga actctttgta ccttctccca 601 gctacttcga tgttgtctac ttgaacccgg acagacaggc tgtggttcct tgtcgggtga 661 ccgtgctgtc ggccaaagtc acgctccaca gggaattccc agccaaggag atcccagcca 721 atggaacgga cattGTTTAT GACATGAAGC GGGGCTTTGT GTATCTGCAA CCTCATTCCG 781 AGCACCAGGG TGTGGTTTAC TGCAGGGCGG AGGCCGGGGG CAGATCTCAG ATCTCCGTCA 841 AGTACCAGCT GCTCTACGTG GCGGTTCCCA GTGGCCCTCC CTCAACAACC ATCTTGGCTT 901 CTTCAAACAA AGTGAAAAGT GGGGACGACA TCAGTGTGCT CTGCACTGTC CTGGGGGAGC 961 CCGATGTGGA GGTGGAGTTC ACCTGGATCT TCCCAGGGCA GAAGGATGAA AGGCCTGTGA 1021 CGATCCAAGA CACTTGGAGG TTGATCCACA GAGGACTGGG ACACACCACG AGAATCTCCC 1081 AGAGTGTCAT TACAGTGGAA GACTTCGAGA CGATTGATGC AGGATATTAC ATTTGCACTG 1141 CTCAGAATCT TCAAGGACAG ACCACAGTAG CTACCACTGT TGAGTTTTCC TGCCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGAACTG CAGCTACAGC AGGAGCACAA ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt