Construct: ORF TRCN0000481583

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015299.1_s317c1
Derived from:
ccsbBroadEn_05440
DNA Barcode:
AAACACTGTCCTCACTCACAGCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DUPD1 (338599)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481583
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 338599 DUPD1 dual specificity phosphatas... NM_001003892.2 100% 100%
2 human 338599 DUPD1 dual specificity phosphatas... XM_011539747.2 100% 100%
3 human 338599 DUPD1 dual specificity phosphatas... XM_017016176.1 44.4% 35.5% (many diffs)
4 mouse 435391 Dupd1 dual specificity phosphatas... NM_001013826.2 83.7% 82.2% (many diffs)
5 mouse 435391 Dupd1 dual specificity phosphatas... XM_006519217.3 83.7% 82.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
729
ORF length:
660
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacatctgga gaagtgaaga caagcctcaa gaatgcctac tcatctgcca
121agaggctgtc gccgaagatg gaggaggaag gggaggagga ggactactgc acccctggag
181cctttgagct ggagcggctc ttctggaagg gcagtcccca gtacacccac gtcaacgagg
241TCTGGCCCAA GCTCTACATT GGCGATGAGG CGACGGCGCT GGACCGCTAT AGGCTGCAGA
301AGGCGGGGTT CACGCACGTG CTGAACGCGG CCCACGGCCG CTGGAACGTG GACACTGGGC
361CCGACTACTA CCGCGACATG GACATCCAGT ACCACGGCGT GGAGGCCGAC GACCTGCCCA
421CCTTCGACCT CAGTGTCTTC TTCTACCCGG CGGCAGCCTT CATCGACAGA GCGCTAAGCG
481ACGACCACAG TAAGATCCTG GTTCACTGCG TCATGGGCCG CAGCCGGTCA GCCACCCTGG
541TCCTGGCCTA CCTGATGATC CACAAGGACA TGACCCTGGT GGACGCCATC CAGCAAGTGG
601CCAAGAACCG CTGCGTCCTC CCGAACCGGG GCTTTTTGAA GCAGCTCCGG GAGCTGGACA
661AGCAGCTGGT GCAGCAGAGG CGACGGTCCC AGCGCCAGGA CGGTGAGGAG GAGGATGGCA
721GGGAGCTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
781CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
841TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAACAC TGTCCTCACT CACAGCGCAC
901GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)