Construct: ORF TRCN0000481596
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000100.1_s317c1
- Derived from:
- ccsbBroadEn_06938
- DNA Barcode:
- ATTCAGGAAAGCCCAAGGATTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFSWAP (6433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481596
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6433 | SFSWAP | splicing factor SWAP | NM_004592.4 | 99.8% | 99.8% | 1262T>C;1635A>G;2514T>C |
| 2 | human | 6433 | SFSWAP | splicing factor SWAP | NM_001261411.2 | 94.7% | 94.7% | (many diffs) |
| 3 | human | 6433 | SFSWAP | splicing factor SWAP | XM_017019798.1 | 91.7% | 91.7% | (many diffs) |
| 4 | human | 6433 | SFSWAP | splicing factor SWAP | XM_011538653.1 | 87% | 87% | (many diffs) |
| 5 | human | 6433 | SFSWAP | splicing factor SWAP | XM_011538655.2 | 85.9% | 82.5% | (many diffs) |
| 6 | human | 6433 | SFSWAP | splicing factor SWAP | XM_011538654.2 | 82% | 81.8% | (many diffs) |
| 7 | human | 6433 | SFSWAP | splicing factor SWAP | XM_024449125.1 | 82% | 81.8% | (many diffs) |
| 8 | human | 6433 | SFSWAP | splicing factor SWAP | XM_024449126.1 | 74.4% | 74.1% | (many diffs) |
| 9 | human | 6433 | SFSWAP | splicing factor SWAP | XM_017019799.1 | 74% | 74% | (many diffs) |
| 10 | mouse | 231769 | Sfswap | splicing factor, suppressor... | NM_172276.3 | 83.6% | 89% | (many diffs) |
| 11 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_011240840.1 | 81.4% | 86.9% | (many diffs) |
| 12 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504300.2 | 79.3% | 84.5% | (many diffs) |
| 13 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504301.2 | 77.3% | 82.5% | (many diffs) |
| 14 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504304.3 | 71.6% | 76.5% | (many diffs) |
| 15 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_017320872.1 | 71.6% | 76.5% | (many diffs) |
| 16 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504305.3 | 69.5% | 74.4% | (many diffs) |
| 17 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504302.3 | 68% | 72.6% | (many diffs) |
| 18 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_006504303.3 | 68% | 72.6% | (many diffs) |
| 19 | mouse | 231769 | Sfswap | splicing factor, suppressor... | XM_011240841.2 | 68% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2922
- ORF length:
- 2853
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtacggcgcg agcgggggcc gcgccaaacc cgagaggaaa agcggcgcga 121 aggaggaggc cgggccaggc ggtgccggcg gtgggggcag ccgagtggag ctcttggttt 181 tcggctatgc ctgcaagctg ttccgggacg acgagcgggc cctggctcag gaacagggac 241 agcacctcat cccctggatg ggggaccaca agatcctcat cgacagatat gatggacgtg 301 gtcacctgca tgacctttct gagtacgatg ctgagtattc cacgtggaac agagattatc 361 agctgtctga agaggaggcg cgaatagagg ccctgtgtga tgaagagagg tatttagcct 421 tgcatacgga cttgcttgag gaggaggcaa ggcaagagga agaatacaag cgattgagtg 481 aagcactagc agaggatggg agctacaatg ccgtggggtt cacttacggt agcgactatt 541 acgacccgtc agagccgacg gaggaggagg agccttccaa acagagagaa aaaaatgagg 601 ccgaaaattt agaggaaaat gaagagccct tcgttgcccc cttaggattg agcgtcccgt 661 ctgacgtgga gttgccacca accgctaaaa tgcacgccat catcgagcgc acggccagct 721 tcgtgtgcag gcagggagca cagtttgaga tcatgctgaa ggccaagcag gcccggaact 781 cccagtttga ctttctgcgc ttcgaccact acctcaaccc ctactataag ttcatccaga 841 aagccatgaa agagggacgc tacactgtcc tggcagaaaa caaaagtgac gagaaaaaaa 901 aatcaggagt cagctctgac aatgaagatg atgatgatga agaagatggg aattaccttc 961 atccctctct ctttgcctcc aagaagtgta accgccttga agagctgatg aagcccttga 1021 aggtagtgga cccagatcat cccctcgcag cacttgttcg taaggcacag gctgacagtt 1081 ccactcccac cccacacaac gcagacggtg cgcctgtgca gccctcccag gtggagtaca 1141 cggcagactc gaccgtggca gccatgtatt acagctacta catgctaccg gacggcactt 1201 actgcctggc gccgccccct cccggaatcg acgtgactac ttactacagc acccttcctg 1261 ctggcgtgac cgtgtctaac tcccctggag tgacgaccac cgccccacca cctcctggga 1321 ccacaccacc accgccccca accacagcag agactagcag cggggccacc tccacaacca 1381 ccaccacaag tgcacttgcc cccgtggccg ccatcatccc cccgcccccc gacgtccagc 1441 ccgtgattga caagctggcc gagtatgtcg ccaggaacgg cctgaagttc gagaccagtg 1501 ttcgtgccaa gaatgatcaa agatttgagt tcctgcagcc gtggcaccag tataatgctt 1561 attatgagtt taagaagcag ttcttcctcc agaaagaagg gggcgatagc atgcaggctg 1621 tgtctgcacc agaagaggct cccacagact ctgctcccga gaagccaagt gatgctgggg 1681 aggatggcgc gcctgaagac gcggccgagg tgggagcacg ggcaggctca ggcgggaaga 1741 aggaggcatc gtccagtaag accgtcccgg acgggaagct ggtgaaagct tcctttgctc 1801 caataagctt tgcaatcaag gccaaagaaa atgatctgct tcccctggaa aaaaatcgtg 1861 ttaagctaga tgatgacagt gatgatgatg aagaaagcaa agaaggccaa gaaagttcta 1921 gtagtgctgc aaacactaac ccagcagttg ccccaccctg tgtagttgtt gaggagaaga 1981 agcctcaact tacccaggag gagctagaag caaagcaagc aaagcaaaag ctggaagatc 2041 gcctcgcagc tgctgcccgg gaaaagctgg cccaggcgtc taaggagtca aaagagaaac 2101 agcttcaagc agaacgtaaa aggaaagcgg cgttattttt acagaccctc aaaaatcctc 2161 tgccggaagc agaagctggg aaaattgagg agagtccttt cagtgtcgag gaatccagca 2221 ctacgccctg ccctctactg actggaggca ggcctctgcc tactttagaa gttaaaccac 2281 ccgataggcc ttcgagcaaa agcaaagatc caccgagaga agaagagaaa gaaaagaaaa 2341 agaaaaagca caaaaaaaga tctcgaacaa gatcacgttc tcccaagtac cattcgtcat 2401 ccaagtccag gtctagatca cactcaaaag caaagcattc tcttcccagt gcctatcgga 2461 cagtgcggcg gtcgaggtcc cgctcccggt cccctcggag gagagcccac tcccctgaga 2521 gacggagGGA AGAGAGGAGT GTGCCCACTG CCTACCGCGT GAGCCGCAGC CCTGGGGCCA 2581 GCAGGAAGCG GACCCGCTCC AGAAGTCCCC ACGAGAAGAA GAAGAAGAGG CGGTCCCGGT 2641 CGCGGACCAA GTCCAAGGCC AGGTCTCAGT CGGTGTCACC CAGCAAGCAG GCAGCGCCCC 2701 GGCCCGCGGC CCCCGCGGCC CACTCGGCGC ACTCAGCCAG CGTCTCCCCT GTGGAGAGTC 2761 GGGGCTCCAG CCAGGAGCGC TCCAGGGGAG TCTCTCAGGA AAAAGAAGCC CAGATCTCTT 2821 CAGCAATCGT TTCTTCCGTG CAGAGCAAAA TCACTCAGGA TCTCATGGCC AAAGTCAGAG 2881 CGATGCTTGC AGCTTCCAAA AACCTGCAAA CCAGCGCTTC CTTGCCAACT TTCTTGTACA 2941 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3001 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3061 AGGACGAATT CAGGAAAGCC CAAGGATTCT GACGCGTTAA GTCgacaatc aacctctgga 3121 ttacaaaatt tgtgaaagat t