Construct: ORF TRCN0000481645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012375.1_s317c1
- Derived from:
- ccsbBroadEn_01114
- DNA Barcode:
- AGGAGCGGCATAAAGAGTTATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP88 (4927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4927 | NUP88 | nucleoporin 88 | NM_002532.6 | 100% | 100% | |
| 2 | human | 4927 | NUP88 | nucleoporin 88 | NM_001320653.1 | 97.8% | 97.7% | 1644_1691del |
| 3 | human | 4927 | NUP88 | nucleoporin 88 | XM_017024694.2 | 82.3% | 82.3% | 0_1ins393 |
| 4 | human | 4927 | NUP88 | nucleoporin 88 | XM_011523893.3 | 80.5% | 80.4% | 0_1ins393;1251_1298del |
| 5 | mouse | 19069 | Nup88 | nucleoporin 88 | NM_172394.3 | 86.7% | 89.9% | (many diffs) |
| 6 | mouse | 19069 | Nup88 | nucleoporin 88 | NM_001276406.1 | 86.1% | 89.3% | (many diffs) |
| 7 | mouse | 19069 | Nup88 | nucleoporin 88 | NM_001083331.2 | 85.4% | 88.5% | (many diffs) |
| 8 | mouse | 19069 | Nup88 | nucleoporin 88 | NR_075087.1 | 57.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2292
- ORF length:
- 2223
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccgcc gagggaccgg tgggcgacgg cgagctgtgg cagacctggc 121 ttcctaacca cgtcgtgttc ttgcggctcc gggagggact gaaaaaccag agtccaaccg 181 aagctgagaa accagcttct tcgtcgttgc cttcgtcgcc gccgccgcag ttgctgacga 241 gaaacgtggt ctttggcctc ggcggagagc ttttcctgtg ggacggagaa gacagctcct 301 tcttagtcgt tcgccttcgg ggccccagcg gcggcggcga agagcccgcc ctgtcccagt 361 accagagatt gctttgcata aatccacccc tgtttgaaat ctatcaagtc ttgttaagcc 421 caacacaaca tcatgtagca cttataggaa taaaaggact tatggtatta gaattaccta 481 aaagatgggg gaagaattct gaatttgaag gtggaaaatc aacagtgaat tgtagtacca 541 ctccagttgc ggagagattt ttcaccagtt ccacctctct gactctaaag catgctgcat 601 ggtatccaag tgaaatcctg gatccccacg tagtgctgtt aacatcagac aacgtaatca 661 gaatttactc actacgtgag ccgcagacac ccactaacgt gataatactt tcagaagccg 721 aagaggaaag tctagtactc aataaaggaa gggcgtatac cgcatctcta ggagagacag 781 cagttgcatt tgactttggg ccattggcag cagtcccaaa gactctattt ggacaaaacg 841 gcaaagatga agtagtggca tacccactgt acatcttata tgaaaatgga gagactttcc 901 tgacatacat cagtctgtta cacagccctg gaaatattgg aaagctgttg ggtccattgc 961 ccatgcatcc tgcggctgaa gataactatg gttatgatgc gtgtgctgta ctctgcttac 1021 cctgtgtccc caatatctta gtgatcgcta ctgaatcagg aatgctgtat cactgtgtcg 1081 tgctagaagg ggaagaagaa gatgaccaca cgtcagaaaa gtcctgggat tccaggattg 1141 acctcattcc ttctctgtat gtgtttgaat gtgttgagtt ggagcttgct ttgaaactgg 1201 catctggaga ggatgaccct tttgattctg acttttcttg tccagtcaaa cttcatagag 1261 atcccaagtg tccttcaaga tatcactgta ctcatgaagc tggtgtacat agtgttgggc 1321 taacttggat tcataaactt cacaaatttc ttggatcaga tgaagaagat aaggatagtt 1381 tacaggaact ctctacagaa cagaaatgct ttgttgaaca catcctttgt acgaagccat 1441 tgccctgcag gcagccagct ccaattcgag gattttggat tgtacctgac attctgggac 1501 ccacgatgat ctgcatcacc agtacctatg aatgcctcat atggccgtta ttaagtacag 1561 tccatccagc gtctcctccc ctgctttgta ctcgagaaga tgttgaagtg gcagagtctc 1621 ccctccgtgt tctggctgaa accccagatt cctttgaaaa gcatattaga agcattttgc 1681 aacgtagtgt tgccaatcca gcatttttga aagcttctga aaaggacata gcccctcctc 1741 ctgaagaatg ccttcagctc ctcagcagag ccacccaggt gttcagagag cagtacattc 1801 tcaaacagga cttggCAAAG GAGGAGATTC AGCGGAGGGT CAAATTATTA TGTGACCAAA 1861 AAAAGAAACA ACTAGAAGAT CTCAGTTATT GTCGAGAAGA GAGGAAAAGT CTGCGGGAAA 1921 TGGCTGAGCG TTTAGCTGAC AAATATGAGG AAGCTAAAGA AAAACAAGAG GATATCATGA 1981 ACAGGATGAA AAAACTACTT CACAGTTTTC ACTCTGAGCT CCCAGTTCTC TCTGATAGTG 2041 AGCGAGACAT GAAGAAAGAA TTACAGCTGA TACCTGATCA ACTTCGACAT TTGGGCAATG 2101 CCATCAAACA GGTTACTATG AAAAAGGATT ATCAACAGCA AAAGATGGAG AAGGTGTTGA 2161 GTCTTCCAAA ACCCACCATT ATTCTCAGTG CCTACCAGCG AAAGTGCATT CAGTCCATCC 2221 TGAAAGAGGA GGGTGAACAT ATAAGGGAAA TGGTGAAGCA AATCAATGAT ATCCGCAATC 2281 ATGTAAACTT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2341 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2401 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGG AGCGGCATAA AGAGTTATTC 2461 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t