Construct: ORF TRCN0000481649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014104.1_s317c1
- Derived from:
- ccsbBroadEn_06022
- DNA Barcode:
- ATGATTGCGGCAATGCGTCGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX7B (1349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1349 | COX7B | cytochrome c oxidase subuni... | NM_001866.3 | 99.5% | 100% | 12G>A |
| 2 | mouse | 66142 | Cox7b | cytochrome c oxidase subuni... | NM_025379.2 | 85.4% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 226
- ORF end:
- 466
- ORF length:
- 240
- Sequence:
-
1 GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA 61 GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT 121 GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT 181 GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGTT TCCCTTAGTC 241 AAAAGCGCAC TAAATCGTCT CCAAGTTCGA AGCATTCAGC AAACAATGGC AAGGCAGAGC 301 CACCAGAAAC GTACACCTGA TTTTCATGAC AAATACGGTA ATGCTGTATT AGCTAGTGGA 361 GCCACTTTCT GTATTGTTAC ATGGACATAT GTAGCAACAC AAGTCGGAAT AGAATGGAAC 421 CTGTCCCCTG TTGGCAGAGT TACCCCAAAG GAATGGAGGA ATCAGTACCC AACTTTCTTG 481 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 541 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 601 GGAAAGGACG AATGATTGCG GCAATGCGTC GCGCAACGCG TTAAGTCgac aatcaacctc 661 tggattacaa aatttgtgaa agatt