Construct: ORF TRCN0000481653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013447.1_s317c1
- Derived from:
- ccsbBroadEn_09526
- DNA Barcode:
- GCCGAATATTACTCTCAGTAGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGPP2 (130367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481653
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130367 | SGPP2 | sphingosine-1-phosphate pho... | NM_152386.4 | 99.7% | 100% | 450A>G;471C>T;945A>G |
| 2 | human | 130367 | SGPP2 | sphingosine-1-phosphate pho... | NM_001320833.2 | 67.6% | 67.9% | (many diffs) |
| 3 | human | 130367 | SGPP2 | sphingosine-1-phosphate pho... | NM_001320834.2 | 67.6% | 67.9% | (many diffs) |
| 4 | human | 130367 | SGPP2 | sphingosine-1-phosphate pho... | XM_017003355.1 | 67.6% | 67.9% | (many diffs) |
| 5 | mouse | 433323 | Sgpp2 | sphingosine-1-phosphate pho... | NM_001004173.2 | 77.4% | 80.2% | (many diffs) |
| 6 | mouse | 433323 | Sgpp2 | sphingosine-1-phosphate pho... | XM_006496506.3 | 65.9% | 69.4% | (many diffs) |
| 7 | mouse | 433323 | Sgpp2 | sphingosine-1-phosphate pho... | XM_017321596.1 | 58.8% | 62.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1266
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgagctg ctgcggagcc tgcaggattc ccagctcgtc gcccgcttcc 121 agcgccgctg cgggctcttc cccgctccgg atgaaggccc ccgggagaac ggcgcggacc 181 ccacggagcg cgcggcgcgg gtccccgggg tcgagcatct ccccgcagcc aacggcaagg 241 gcggcgaggc tccggccaac gggctgcgca gagccgcggc gccggaggct tatgtacaga 301 agtacgtcgt gaagaattat ttctactatt acctattcca attttcagct gctttgggcc 361 aagaagtgtt ctacatcacg tttcttccat tcactcactg gaatattgac ccttatttat 421 ccagaagatt gatcatcata tgggttttgg tgatgtatat tggccaagtg gccaaggatg 481 tcttgaagtg gccccgtccc tcctcccctc cagttgtgaa actggaaaag agactgattg 541 ctgaatatgg aatgccatcc acccacgcca tggcggccac tgccattgcc ttcaccctcc 601 ttatctctac tatggacaga taccagtatc catttgtgtt gggactggtg atggccgtgg 661 tgttttccac cttggtgtgt ctcagcaggc tctacactgg gatgcatacg gtcctggatg 721 tgctgggtgg cgtcctgatc accgcactcc tcatcgtcct cacctaccct gcctggacct 781 tcatcgactg cctggactcg gccagccccc tcttccccgt gtgtgtcata gttgtgccat 841 tcttcctgtg ttacaattac cctgtttctg attacTACAG CCCAACCCGG GCGGACACCA 901 CCACCATTCT GGCTGCCGGG GCTGGAGTGA CCATAGGATT CTGGATCAAC CATTTCTTCC 961 AGCTTGTATC CAAGCCCGCT GAATCTCTCC CTGTTATTCA GAACATCCCA CCGCTCACCA 1021 CCTACATGTT AGTTTTGGGT CTGACCAAAT TTGCAGTGGG AATTGTGTTG ATCCTCTTGG 1081 TTCGTCAGCT TGTACAAAAT CTCTCACTGC AAGTATTATA CTCATGGTTC AAGGTGGTCA 1141 CCAGGAACAA GGAGGCCAGG CGGAGACTGG AGATTGAAGT GCCTTACAAG TTTGTTACCT 1201 ACACATCTGT TGGCATCTGC GCTACAACCT TTGTGCCGAT GCTTCACAGG TTTCTGGGAT 1261 TACCCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCGAATAT TACTCTCAGT AGGGCACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt