Construct: ORF TRCN0000487686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019069.1_s317c1
- DNA Barcode:
- GCTTCCTAGCCATACGCAGCGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA1 (6195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_002953.4 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001330441.2 | 96.1% | 95.7% | (many diffs) |
| 3 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001006665.2 | 95.7% | 95.1% | (many diffs) |
| 4 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | XM_024448871.1 | 87.3% | 87.3% | (many diffs) |
| 5 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_009097.5 | 90.9% | 98.3% | (many diffs) |
| 6 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285505.1 | 89.4% | 96.8% | (many diffs) |
| 7 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | XM_006538677.3 | 87.4% | 92.8% | (many diffs) |
| 8 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285506.1 | 86.7% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2280
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgctc gcccagctca aggagccctg gccgctcatg gagctagtgc 121 cgctggaccc ggagaatgga cagacctcag gggaagaagc tggacttcag ccgtccaagg 181 atgagggcgt cctcaaggag atctccatca cgcaccacgt caaggctggc tctgagaagg 241 ctgatccatc ccatttcgag ctcctcaagg ttctgggcca gggatccttt ggcaaagtct 301 tcctggtgcg gaaagtcacc cggcctgaca gtgggcacct gtatgctatg aaggtgctga 361 agaaggcaac gctgaaagta cgtgaccgcg tccggaccaa gatggagaga gacatcctgg 421 ctgatgtaaa tcacccattc gtggtgaagc tgcactatgc cttccagacc gagggcaagc 481 tctatctcat tctggacttc ctgcgtggtg gggacctctt cacccggctc tcaaaagagg 541 tgatgttcac ggaggaggat gtgaagtttt acctggccga gctggctctg ggcctggatc 601 acctgcacag cctgggtatc atttacagag acctcaagcc tgagaacatc cttctggatg 661 aggagggcca catcaaactc actgactttg gcctgagcaa agaggccatt gaccacgaga 721 agaaggccta ttctttctgc gggacagtgg agtacatggc ccctgaggtc gtcaaccgcc 781 agggccactc ccatagtgcg gactggtggt cctatggggt gttgatgttt gagatgctga 841 cgggctccct gcccttccag gggaaggacc ggaaggagac catgacactg attctgaagg 901 cgaagctagg catgccccag tttctgagca ctgaagccca gagcctcttg cgggccctgt 961 tcaagcggaa tcctgccaac cggctcggct ccggccctga tggggcagag gaaatcaagc 1021 ggcatgtctt ctactccacc attgactgga ataagctata ccgtcgtgag atcaagccac 1081 ccttcaagcc agcagtggct cagcctgatg acaccttcta ctttgacacc gagttcacgt 1141 cccgcacacc caaggattcc ccaggcatcc cccccagcgc tggggcccat cagctgttcc 1201 ggggcttcag cttcgtggcc accggcctga tggaagacga cggcaagcct cgtgccccgc 1261 aggcacccct gcactcggtg gtacagcaac tccatgggaa gaacctggtt tttagtgacg 1321 gctacgtggt aaaggagaca attggtgtgg gctcctactc tgagtgcaag cgctgtgtcc 1381 acaaggccac caacatggag tatgctgtca aggtcattga taagagcaag cgggatcctt 1441 cagaagagat tgagattctt ctgcggtatg gccagcaccc caacatcatc actctgaaag 1501 atgtgtatga tgatggcaaa cacgtgtacc tggtgacaga gctgatgcgg ggtggggagc 1561 tgctggacaa gatcctgcgg cagaagttct tctctgagcg ggaggccagc tttgtcctgc 1621 acaccattgg caaaactgtg gagtatctgc actcacaggg ggttgtgcac agggacctga 1681 agcccagcaa catcctgtat gtggacgagt ccgggaatcc cgagtgcctg cgcatctgtg 1741 actttggttt tgccaaacag ctgcgggctg agaatgggct cctcatgaca ccttgctaca 1801 cagccaactt tgtggcgcct gaggtgctga agcgccaggg ctacgatgaa ggctgcgaca 1861 tctggagcct gggcattctg ctgtacacca tgctggcagg atatactcca tttgccaacg 1921 gtcccagtga cacaccagag gaaatcctaa cccggatcgg cagtgggaag tttaccctca 1981 gtgggggaaa ttggaacaca gtttcagaga cagccaagga cctggtgtcc aagatgctac 2041 acgtggatcc ccaccagcgc ctcACAGCTA AGCAGGTTCT GCAGCATCCA TGGGTCACCC 2101 AGAAAGACAA GCTTCCCCAA AGCCAGCTGT CCCACCAGGA CCTACAGCTT GTGAAGGGAG 2161 CCATGGCTGC CACGTACTCC GCACTCAACA GCTCCAAGCC CACCCCCCAG CTGAAGCCCA 2221 TCGAGTCATC CATCCTGGCC CAGCGGCGAG TGAGGAAGTT GCCATCCACC ACCCTGGACC 2281 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GAGCTTCCTA GCCATACGCA GCGCCAACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt