Construct: ORF TRCN0000487689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020197.1_s317c1
- DNA Barcode:
- CGAGGCACTCAGAAAACCACCCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA10 (4705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_004544.4 | 99.8% | 100% | 105A>G;771A>G |
| 2 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | XM_011511228.3 | 94.4% | 92% | (many diffs) |
| 3 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_001322019.1 | 89.1% | 87% | (many diffs) |
| 4 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_001322020.2 | 89.1% | 84.8% | (many diffs) |
| 5 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | XR_001738750.2 | 62.1% | (many diffs) | |
| 6 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136158.2 | 19.5% | (many diffs) | |
| 7 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136155.2 | 13.4% | (many diffs) | |
| 8 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136156.2 | 11.9% | (many diffs) | |
| 9 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136157.2 | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1137
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccttg cggctcctga agctggcagc gacgtccgcg tccgcccggg 121 tcgtggcggc gggcgcccag cgcgtgagag gaattcatag cagtgtgcag tgcaagctgc 181 gctatggaat gtggcatttc ctacttgggg ataaagcaag caaaagactg acagaacgca 241 gcagagtgat aactgtagat ggcaatatat gtactggaaa aggcaaactt gcaaaagaaa 301 tagcagagaa actaggcttc aagcactttc ctgaagcggg gattcattat ccagacagta 361 ccacaggaga tgggaagccc ctcgccaccg actataatgg caactgtagt ttggagaaat 421 tttacgatga tccgagaagc aatgatggca acagttaccg cctgcagtcc tggttgtaca 481 gcagtcgcct gctgcagtac tcagatgcct tggagcactt gctgaccaca ggacaaggtg 541 ttgtgttgga gcgctccatc ttcagtgact ttgtgttcct ggaggcgatg tacaaccagg 601 gattcatccg aaagcagtgt gtggaccact acaacgaggt gaagagcgtc accatctgcg 661 attacctgcc cccccacctg gtgatttaca tcgatgtgcc cgttccagag gtccagaggc 721 ggattcagaa gaaaggagat ccacatgaaa tgaagatcac ctctgcctat ctacaggaca 781 ttgagaatgc ctataagaaa acctttctcc ctgagatgag tgaaaaatgt gaggttttac 841 agtattctgc aagggaagct caagattcaa aaaaggtggt agaggacatt gaataccTGA 901 AGTTCGATAA AGGGCCGTGG CTCAAGCAGG ACAATCGCAC TTTATACCAC CTGCGATTAC 961 TGGTTCAGGA TAAGTTTGAG GTGCTGAATT ACACAAGCAT TCCTATCTTT CTCCCGGAAG 1021 TCACCATTGG AGCTCATCAG ACTGACCGTG TCTTACATCA GTTCAGAGAG CTGCCGGGCC 1081 GCAAGTACAG CCCTGGGTAC AACACCGAGG TGGGAGACAA GTGGATCTGG CTGAAGTGAG 1141 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGACGAGG CACTCAGAAA ACCACCCACA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt