Transcript: Human NM_004544.4

Homo sapiens NADH:ubiquinone oxidoreductase subunit A10 (NDUFA10), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
NDUFA10 (4705)
Length:
4855
CDS:
42..1109

Additional Resources:

NCBI RefSeq record:
NM_004544.4
NBCI Gene record:
NDUFA10 (4705)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004544.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027175 GCGCTATGGAATGTGGCATTT pLKO.1 149 CDS 100% 10.800 15.120 N NDUFA10 n/a
2 TRCN0000027164 CCGACTATAATGGCAACTGTA pLKO.1 358 CDS 100% 4.950 6.930 N NDUFA10 n/a
3 TRCN0000027176 CGCAGCAGAGTGATAACTGTA pLKO.1 207 CDS 100% 4.950 3.960 N NDUFA10 n/a
4 TRCN0000027210 GCAGGACAATCGCACTTTATA pLKO.1 896 CDS 100% 15.000 10.500 N NDUFA10 n/a
5 TRCN0000428159 GAATACCTGAAGTTCGATAAA pLKO_005 861 CDS 100% 13.200 9.240 N NDUFA10 n/a
6 TRCN0000431199 TACTGGTTCAGGATAAGTTTG pLKO_005 928 CDS 100% 10.800 7.560 N NDUFA10 n/a
7 TRCN0000027168 GTGCTGAATTACACAAGCATT pLKO.1 951 CDS 100% 4.950 3.465 N NDUFA10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004544.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06622 pDONR223 100% 99.8% 100% None 105A>G;771A>G n/a
2 ccsbBroad304_06622 pLX_304 0% 99.8% 100% V5 105A>G;771A>G n/a
3 TRCN0000470535 CTGACGTGCCCTAATTCCCTGTGC pLX_317 52.2% 99.8% 100% V5 105A>G;771A>G n/a
4 TRCN0000487689 CGAGGCACTCAGAAAACCACCCAC pLX_317 23.1% 99.8% 100% V5 (not translated due to prior stop codon) 105A>G;771A>G n/a
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