Construct: ORF TRCN0000487694
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021966.1_s317c1
- DNA Barcode:
- CAAGCTGGTCTTCCAATCGACGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SYK (6850)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487694
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174167.3 | 99.7% | 100% | (many diffs) |
| 2 | human | 6850 | SYK | spleen associated tyrosine ... | NM_003177.7 | 99.7% | 100% | (many diffs) |
| 3 | human | 6850 | SYK | spleen associated tyrosine ... | XM_005252147.4 | 99.7% | 100% | (many diffs) |
| 4 | human | 6850 | SYK | spleen associated tyrosine ... | XM_011518946.3 | 99.7% | 100% | (many diffs) |
| 5 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001135052.4 | 96.1% | 96.3% | (many diffs) |
| 6 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174168.3 | 96.1% | 96.3% | (many diffs) |
| 7 | human | 6850 | SYK | spleen associated tyrosine ... | XR_001746370.2 | 37.5% | (many diffs) | |
| 8 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_001198977.1 | 84.6% | 92.1% | (many diffs) |
| 9 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_011518.2 | 84.6% | 92.1% | (many diffs) |
| 10 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516895.4 | 84.6% | 92.1% | (many diffs) |
| 11 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516897.4 | 81.6% | 88.8% | (many diffs) |
| 12 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_017315471.2 | 47.5% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1974
- ORF length:
- 1905
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccagcagc ggcatggctg acagcgccaa ccacctgccc ttctttttcg 121 gcaacatcac ccgggaggag gcagaagatt acctggtcca ggggggcatg agtgatgggc 181 tttatttgct gcgccagagc cgcaactacc tgggtggctt cgccctgtcc gtggcccacg 241 ggaggaaggc acaccactac accatcgagc gggagctgaa tggcacctac gccatcgccg 301 gtggcaggac ccatgccagc cccgccgacc tctgccacta ccactcccag gagtctgatg 361 gcctggtctg cctcctcaag aagcccttca accggcccca aggggtgcag cccaagactg 421 ggccctttga ggatttgaag gaaaacctca tcagggaata tgtgaagcag acatggaacc 481 tgcagggtca ggctctggag caggccatca tcagtcagaa gcctcagctg gagaagctga 541 tcgctaccac agcccatgaa aaaatgcctt ggttccatgg aaaaatctct cgggaagaat 601 ctgagcaaat tgtcctgata ggatcaaaga caaatggaaa gttcctgatc cgagccagag 661 acaacaacgg ctcctacgcc ctgtgcctgc tgcacgaagg gaaggtgctg cactatcgca 721 tcgacaaaga caagacaggg aagctctcca tccccgaggg aaagaagttc gacacgctct 781 ggcagctagt cgagcattat tcttataaag cagatggttt gttaagagtt cttactgtcc 841 catgtcaaaa aatcggcaca cagggaaatg ttaattttgg aggccgtcca caacttccag 901 gttcccatcc tgcgacttgg tcagcgggtg gaataatctc aagaatcaaa tcatactcct 961 tcccaaagcc tggccacaga aagtcctccc ctgcccaagg gaaccggcaa gagagtactg 1021 tgtcattcaa tccgtatgag ccagaacttg caccctgggc tgcagacaaa ggcccccaga 1081 gagaagccct acccatggac acagaggtgt acgagagccc ctacgcggac cctgaggaga 1141 tcaggcccaa ggaggtttac ctggaccgaa agctgctgac gctggaagac aaagaactgg 1201 gctctggtaa ttttggaact gtgaaaaagg gctactacca aatgaaaaaa gttgtgaaaa 1261 ccgtggctgt gaaaatactg aaaaacgagg ccaatgaccc cgctcttaaa gatgagttat 1321 tagcagaagc aaatgtcatg cagcagctgg acaacccgta catcgtgcgc atgatcggga 1381 tatgcgaggc cgagtcctgg atgctagtta tggagatggc agaacttggt cccctcaata 1441 agtatttgca gcagaacaga catgtcaagg ataagaacat catagaactg gttcatcagg 1501 tttccatggg catgaagtac ttggaggaga gcaattttgt gcacagagat ctggctgcaa 1561 gaaatgtgtt gctagttacc caacattatg ccaagatcag tgatttcgga ctctccaaag 1621 cactgcgtgc tgatgaaaac tactacaagg cccagaccca tggaaagtgg cctgtcaagt 1681 ggtacgctcc ggaatgcatc aactactaca agttctccag caaaagcgat gtctggagct 1741 ttggagtgtt gatgtgggaa gcatTCTCCT ATGGGCAGAA GCCATATCGA GGGATGAAAG 1801 GAAGTGAAGT CACCGCTATG TTAGAGAAAG GAGAGCGGAT GGGGTGCCCT GCAGGGTGTC 1861 CAAGAGAGAT GTACGATCTC ATGAATCTGT GCTGGACATA CGATGTGGAA AACAGGCCCG 1921 GATTCGCAGC AGTGGAACTG CGGCTGCGCA ATTACTACTA TGACGTGGTG AACTAGAACC 1981 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2041 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2101 ATATATCTTG TGGAAAGGAC GACAAGCTGG TCTTCCAATC GACGTGACGC GTTAAGTCga 2161 caatcaacct ctggattaca aaatttgtga aagatt