Construct: ORF TRCN0000487701
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021132.1_s317c1
- DNA Barcode:
- CCGTGAGCCAAGAATTGAAACCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NPFFR1 (64106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487701
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64106 | NPFFR1 | neuropeptide FF receptor 1 | NM_022146.5 | 99.9% | 100% | 597G>T |
| 2 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | NM_001177511.1 | 86% | 86.5% | (many diffs) |
| 3 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | XM_011243444.2 | 62.5% | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1362
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagggg gagccctccc agcctcccaa cagcagttgg cccctaagtc 121 agaatgggac taacactgag gccaccccgg ctacaaacct caccttctcc tcctactatc 181 agcacacctc ccctgtggcg gccatgttca ttgtggccta tgcgctcatc ttcctgctct 241 gcatggtggg caacaccctg gtctgtttca tcgtgctcaa gaaccggcac atgcatactg 301 tcaccaacat gttcatcctc aacctggctg tcagtgacct gctggtgggc atcttctgca 361 tgcccaccac ccttgtggac aacctcatca ctgggtggcc cttcgacaat gccacatgca 421 agatgagcgg cttggtgcag ggcatgtctg tgtcggcttc cgttttcaca ctggtggcca 481 ttgctgtgga aaggttccgc tgcatcgtgc accctttccg cgagaagctg accctgcgga 541 aggcgctcgt caccatcgcc gtcatctggg ccctggcgct gctcatcatg tgtccctcgg 601 ccgtcacgct gaccgtcacc cgtgaggagc accacttcat ggtggacgcc cgcaaccgct 661 cctaccctct ctactcctgc tgggaggcct ggcccgagaa gggcatgcgc agggtctaca 721 ccactgtgct cttctcgcac atctacctgg cgccgctggc gctcatcgtg gtcatgtacg 781 cccgcatcgc gcgcaagctc tgccaggccc cgggcccggc ccccgggggc gaggaggctg 841 cggacccgcg agcatcgcgg cgcagagcgc gcgtggtgca catgctggtc atggtggcgc 901 tgttcttcac gctgtcctgg ctgccgctct gggcgctgct gctgctcatc gactacgggc 961 agctcagcgc gccgcagctg cacctggtca ccgtctacgc cttccccttc gcgcactggc 1021 tggccttctt caacagcagc gccaacccca tcatctacgg ctacttcaac gagaacttcc 1081 gccgcggctt ccaggccgcc ttccgcgccc gcctctgccc gcgcccgtcg gGGAGCCACA 1141 AGGAGGCCTA CTCCGAGCGG CCCGGCGGGC TTCTGCACAG GCGGGTCTTC GTGGTGGTGC 1201 GGCCCAGCGA CTCCGGGCTG CCCTCTGAGT CGGGCCCTAG CAGTGGGGCC CCCAGGCCCG 1261 GCCGCCTCCC GCTGCGGAAT GGGCGGGTGG CTCACCACGG CTTGCCCAGG GAAGGGCCTG 1321 GCTGCTCCCA CCTGCCCCTC ACCATTCCAG CCTGGGATAT CTGAGACCCA GCTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA CCGTGAGCCA AGAATTGAAA CCCCACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt