Construct: ORF TRCN0000487707
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021458.1_s317c1
- DNA Barcode:
- TGCGATTCTGGCCGACCTTATCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GNAL (2774)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487707
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2774 | GNAL | G protein subunit alpha L | NM_001142339.2 | 99.3% | 100% | 1143_1144insTGAAAGCT |
| 2 | human | 2774 | GNAL | G protein subunit alpha L | NM_001261443.1 | 99.3% | 100% | 1143_1144insTGAAAGCT |
| 3 | human | 2774 | GNAL | G protein subunit alpha L | NM_001369387.1 | 99.3% | 100% | 1143_1144insTGAAAGCT |
| 4 | human | 2774 | GNAL | G protein subunit alpha L | NM_182978.4 | 78.6% | 75.5% | (many diffs) |
| 5 | human | 2774 | GNAL | G protein subunit alpha L | NM_001261444.2 | 45.3% | 45.6% | 0_1ins621;522_523insTGAAAGCT |
| 6 | human | 2774 | GNAL | G protein subunit alpha L | XM_006722324.3 | 31.7% | 27.9% | (many diffs) |
| 7 | mouse | 14680 | Gnal | guanine nucleotide binding ... | NM_010307.3 | 90.7% | 99.2% | (many diffs) |
| 8 | mouse | 14680 | Gnal | guanine nucleotide binding ... | NM_177137.5 | 74.4% | 78.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatggg gtgtttgggc ggcaacagca agacgacgga agaccagggc gtcgatgaaa 121 aagaacgacg cgaggccaac aaaaagatcg agaagcagtt gcagaaagag cgcctggctt 181 acaaggctac ccaccgcctg ctgctcctgg gggctggtga gtctgggaaa agcactatcg 241 tcaaacagat gaggatcctg cacgtcaatg ggtttaatcc cgaggaaaag aaacagaaaa 301 ttctggacat ccggaaaaat gttaaagatg ctatcgtgac aattgtttca gcaatgagta 361 ctataatacc tccagttccg ctggccaacc ctgaaaacca atttcgatca gactacatca 421 agagcatagc ccctatcact gactttgaat attcccagga attctttgac catgtgaaaa 481 aactttggga cgatgaaggc gtgaaggcat gctttgagag atccaacgaa taccagctga 541 ttgactgtgc acaatacttc ctggaaagaa tcgacagcgt cagcttggtt gactacacac 601 ccacagacca ggacctcctc agatgcagag ttctgacatc tgggattttt gagacacgat 661 tccaagtgga caaagtaaac ttccacatgt ttgatgttgg tggccagagg gatgagagga 721 gaaaatggat ccagtgcttt aacgatgtca cagctatcat ttacgtcgca gcctgcagta 781 gctacaacat ggtgattcga gaagataaca acaccaacag gctgagagag tccctggatc 841 tttttgaaag catctggaac aacaggtggt tacggaccat ttctatcatc ttgttcttga 901 acaaacaaga tatgctggca gaaaaagtct tGGCAGGGAA ATCAAAAATT GAAGACTATT 961 TCCCAGAATA TGCAAATTAT ACTGTTCCTG AAGACGCAAC ACCAGATGCA GGAGAAGATC 1021 CCAAAGTTAC AAGAGCCAAG TTCTTTATCC GGGACCTGTT TTTGAGGATC AGCACGGCCA 1081 CCGGTGACGG CAAACATTAC TGCTACCCGC ACTTCACCTG CGCCGTGGAC ACAGAGAACA 1141 TCCGCAGGGT GTTCAACGAC TGCCGCGACA TCATCCAGCG GATGCACCTC AAGCAGTATG 1201 AGCTCTTGTG AAAGCTTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCGATT CTGGCCGACC 1381 TTATCACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt