Construct: ORF TRCN0000487710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020266.1_s317c1
- DNA Barcode:
- CAAGATAAGCGTCGGCCCTTGGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RBKS (64080)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64080 | RBKS | ribokinase | NM_022128.3 | 99.8% | 100% | 673T>C |
| 2 | human | 64080 | RBKS | ribokinase | NM_001287580.1 | 79% | 79.1% | 0_1ins201;472T>C |
| 3 | mouse | 71336 | Rbks | ribokinase | NM_153196.1 | 85.4% | 86% | (many diffs) |
| 4 | mouse | 71336 | Rbks | ribokinase | XM_006504114.1 | 71.2% | 71.5% | (many diffs) |
| 5 | mouse | 71336 | Rbks | ribokinase | XM_006504113.3 | 70.1% | 69.6% | (many diffs) |
| 6 | mouse | 71336 | Rbks | ribokinase | XR_376828.2 | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1038
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcg tctggggaac cccagaggca gtggcaagag gaggtggcgg 121 cggtggtagt ggtgggctcc tgcatgaccg acctggtcag tcttacttct cgtttgccaa 181 aaactggaga aaccatccat ggacataagt tttttattgg ctttggaggg aaaggtgcca 241 accagtgtgt ccaagctgct cggcttggag caatgacgtc catggtgtgt aaggttggca 301 aagattcttt tggcaatgat tatatagaaa acttaaaaca gaatgatatt tctacagaat 361 ttacatatca gactaaagat gctgctacag gaactgcttc tataattgtc aataatgaag 421 gccagaatat cattgtcata gtggctggag caaatttact tttgaatacg gaggatctga 481 gggcagcagc caatgtcatt agcagagcca aagtcatggt ctgccagctc gaaataactc 541 cagcaacttc tttggaagcc ctaacaatgg cccgcaggag tggagtgaaa accttgttca 601 atccagcccc tgccattgct gacctggatc cccagttcta caccctctca gatgtgttct 661 gctgcaatga aagtgaggct gagattttaa ctggcctcac ggtgggcagc gctgcagatg 721 ctggggaggc tgcattagtg ctcctgaaaa ggggctgcca ggtggtaatc attaccttag 781 gggctgaagg atgtgtggtg ctgtcacaga cagaaccTGA GCCAAAGCAC ATTCCCACAG 841 AGAAAGTCAA GGCTGTGGAT ACCACGGGTG CTGGTGACAG CTTTGTGGGA GCTCTGGCCT 901 TCTACCTGGC TTACTATCCA AATCTGTCCT TGGAAGACAT GCTCAACAGA TCCAATTTCA 961 TTGCAGCAGT CAGTGTCCAG GCTGCAGGAA CACAGTCATC TTACCCTTAC AAAAAAGACC 1021 TTCCGCTTAC TCTGTTTTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAG ATAAGCGTCG 1201 GCCCTTGGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt