Construct: ORF TRCN0000487719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021011.1_s317c1
- DNA Barcode:
- CCAGTAACCCTTTATCGGCGTGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADRA1B (147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147 | ADRA1B | adrenoceptor alpha 1B | NM_000679.4 | 99.9% | 100% | 1261C>T |
| 2 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_011534435.1 | 93.4% | 93.5% | 949_1056del;1369C>T |
| 3 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_011534437.2 | 68.2% | 63.8% | (many diffs) |
| 4 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_011534438.2 | 68.1% | 63.3% | (many diffs) |
| 5 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_005265818.3 | 64% | 61.6% | (many diffs) |
| 6 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_005265819.2 | 62.3% | 61.5% | (many diffs) |
| 7 | human | 147 | ADRA1B | adrenoceptor alpha 1B | XM_006714821.3 | 62.3% | 61.5% | (many diffs) |
| 8 | mouse | 11548 | Adra1b | adrenergic receptor, alpha 1b | NM_001284380.1 | 89.8% | 95.3% | (many diffs) |
| 9 | mouse | 11548 | Adra1b | adrenergic receptor, alpha 1b | NM_001284381.1 | 89.8% | 95.3% | (many diffs) |
| 10 | mouse | 11548 | Adra1b | adrenergic receptor, alpha 1b | NM_007416.4 | 89.8% | 95.3% | (many diffs) |
| 11 | mouse | 11548 | Adra1b | adrenergic receptor, alpha 1b | XM_011248675.2 | 89.8% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1632
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatccc gacctggaca ccggccacaa cacatcagca cctgcccact 121 ggggagagtt gaaaaatgcc aacttcactg gccccaacca gacctcgagc aactccacac 181 tgccccagct ggacatcacc agggccatct ctgtgggcct ggtgctgggc gccttcatcc 241 tctttgccat cgtgggcaac atcctagtca tcttgtctgt ggcctgcaac cggcacctgc 301 ggacgcccac caactacttc attgtcaacc tggccatggc cgacctgctg ttgagcttca 361 ccgtcctgcc cttctcagcg gccctagagg tgctcggcta ctgggtgctg gggcggatct 421 tctgtgacat ctgggcagcc gtggatgtcc tgtgctgcac agcgtccatt ctgagcctgt 481 gcgccatctc catcgatcgc tacatcgggg tgcgctactc tctgcagtat cccacgctgg 541 tcacccggag gaaggccatc ttggcgctgc tcagtgtctg ggtcttgtcc accgtcatct 601 ccatcgggcc tctccttggg tggaaggagc cggcacccaa cgatgacaag gagtgcgggg 661 tcaccgaaga acccttctat gccctcttct cctctctggg ctccttctac atccctctgg 721 cggtcattct agtcatgtac tgccgtgtct atatagtggc caagagaacc accaagaacc 781 tagaggcagg agtcatgaag gagatgtcca actccaagga gctgaccctg aggatccatt 841 ccaagaactt tcacgaggac acccttagca gtaccaaggc caagggccac aaccccagga 901 gttccatagc tgtcaaactt tttaagttct ccagggaaaa gaaagcagct aagacgttgg 961 gcattgtggt cggtatgttc atcttgtgct ggctaccctt cttcatcgct ctaccgcttg 1021 gctccttgtt ctccaccctg aagccccccg acgccgtgtt caaggtggtg ttctggctgg 1081 gctacttcaa cagctgcctc aaccccatca tctacccatg ctccagcaag gagttcaagc 1141 gcgctttcgt gcgcatcctc gggtgccagt gccgcggccg cggccgccgc cgacgccgcc 1201 gccgccgtcg cctgggcggc tgcgcctaca cctaccggcc gtggacgcgc ggcggctcgc 1261 tggagcgctc gcagtcgcgc aaggactcgc tggacgacag cggcagctgc ctgagcggca 1321 gccagcggac cttgccctcg gcctcgccga gcccgggcta ccTGGGCCGC GGCGCGCCAC 1381 CGCCAGTCGA GCTGTGCGCC TTCCCCGAGT GGAAGGCGCC CGGCGCCCTC CTGAGCCTGC 1441 CCGCGCCTGA GCCCCCCGGC CGCCGCGGCC GCCACGACTC GGGCCCGCTC TTCACCTTCA 1501 AGCTCCTGAC CGAGCCCGAG AGCCCCGGGA CCGACGGCGG CGCCAGCAAC GGAGGCTGCG 1561 AGGCCGCGGC CGACGTGGCC AACGGGCAGC CGGGCTTCAA AAGCAACATG CCCCTGGCGC 1621 CCGGGCAGTT TTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCAGTAACCC TTTATCGGCG 1801 TGAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt