Construct: ORF TRCN0000487726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021276.1_s317c1
- DNA Barcode:
- GTCGCGGCATACTTTTCTTTACAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TOE1 (114034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114034 | TOE1 | target of EGR1, exonuclease | NM_025077.4 | 100% | 100% | |
| 2 | human | 114034 | TOE1 | target of EGR1, exonuclease | XM_005270412.4 | 98.8% | 98.8% | 52_69del |
| 3 | human | 114034 | TOE1 | target of EGR1, exonuclease | XM_005270413.5 | 90.9% | 90.9% | 0_1ins138 |
| 4 | human | 114034 | TOE1 | target of EGR1, exonuclease | XM_024452837.1 | 86% | 86% | 0_1ins138;57_143del |
| 5 | human | 114034 | TOE1 | target of EGR1, exonuclease | XR_946532.3 | 76.3% | (many diffs) | |
| 6 | human | 114034 | TOE1 | target of EGR1, exonuclease | XR_426587.4 | 76.1% | (many diffs) | |
| 7 | human | 114034 | TOE1 | target of EGR1, exonuclease | XM_011540569.3 | 75% | 75% | 0_1ins381 |
| 8 | human | 114034 | TOE1 | target of EGR1, exonuclease | XR_001736951.2 | 73.3% | 1_187del;939_1042del;1822_2087del | |
| 9 | human | 114034 | TOE1 | target of EGR1, exonuclease | XR_246230.4 | 72.8% | 1_187del;939_1046del;1826_2099del | |
| 10 | human | 114034 | TOE1 | target of EGR1, exonuclease | XR_002959287.1 | 61.1% | (many diffs) | |
| 11 | mouse | 68276 | Toe1 | target of EGR1, member 1 (n... | NM_026654.2 | 83.5% | 81.6% | (many diffs) |
| 12 | mouse | 68276 | Toe1 | target of EGR1, member 1 (n... | XR_376349.2 | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1602
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcc gacagtgacg atggcgcagt ttcagctccc gcagcttccg 121 acggtggtgt cagcaaaagc acaacatctg gggaggagct agtagtccag gttcccgtag 181 tggatgtgca aagcaacaac ttcaaggaga tgtggccatc cctcctgcta gccataaaga 241 cagctaattt cgtggctgtg gacacggagc tgagtgggct tggggacagg aagagtttgc 301 tgaaccagtg cattgaggaa cgttacaagg ccgtgtgtca tgctgccagg acccgttcta 361 tcctttccct gggcctcgcc tgcttcaagc ggcagccaga caagggtgaa cattcctatc 421 tggctcaagt gttcaatctc actctgctgt gcatggagga gtatgtcata gaaccaaagt 481 ctgtgcagtt cctgatacag catggcttca acttcaacca gcagtatgcc caaggcatcc 541 cctaccataa gggcaatgac aagggtgatg agagccagag ccagtcagta cggaccctat 601 tcctggagct aatccgagcc cgccggcccc tggtgctaca caatggcctt atagacttgg 661 tgttcctgta ccagaacttc tatgcacacc tccctgagag tctgggaacc ttcaccgctg 721 acctgtgtga gatgttccca gcaggcattt atgacaccaa atatgctgct gagtttcatg 781 cccgtttcgt ggcctcctac ttagaatatg ccttccggaa atgtgaacgg gaaaatggga 841 agcagcgggc agctggcagc ccacacctta ccctggagtt ctgcaactat ccttccagca 901 tgagggacca tattgattac cgctgctgcc tgcccccagc aacccaccgt cctcatccca 961 ccagcatctg tgacaacttc tcggcttatg gctggtgccc cctgggacca cagtgtcctc 1021 agtctcacga tattgacctt atcattgaca ctgatgaggc tgcggcagag gacaagcggc 1081 gacggcgacg acgtagggaa aaacggaaga gggctttatt gaacctaccg gggacacaga 1141 cctctgggga agctaaggat ggtcctccca agaagcaggt ctgtggggat agcatcaagc 1201 ctgaagaaac cgagcaggag gtggctgccg atgaaactag gaacctgcct cactccaagc 1261 aaggcaacaa aaatgactta gagatgggga ttaaggcagc aaggcctgaa atagctgata 1321 gagctaccTC AGAAGTGCCA GGGAGCCAAG CCAGTCCTAA CCCAGTGCCT GGGGATGGAT 1381 TGCACCGGGC TGGTTTTGAT GCCTTTATGA CAGGTTATGT GATGGCCTAT GTGGAAGTGA 1441 GCCAGGGACC GCAGCCCTGC AGCTCTGGAC CCTGGCTCCC TGAATGCCAC AATAAGGTAT 1501 ATTTGAGTGG CAAAGCTGTA CCCCTCACAG TGGCCAAGAG CCAGTTCTCT CGTTCCTCCA 1561 AAGCCCACAA TCAGAAGATG AAGCTCACTT GGGGCAGTAG CTAGAACCCA GCTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA GTCGCGGCAT ACTTTTCTTT ACAGACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt