Construct: ORF TRCN0000487732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019466.1_s317c1
- DNA Barcode:
- GTACTTCCTAGACCAAGAACGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RY12 (64805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_022788.4 | 99.7% | 99.7% | 18C>T;36T>G;1026_1027insG |
| 2 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_176876.3 | 99.7% | 99.7% | 18C>T;36T>G;1026_1027insG |
| 3 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | NM_027571.3 | 82% | 85.9% | (many diffs) |
| 4 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502083.3 | 82% | 85.9% | (many diffs) |
| 5 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502084.3 | 82% | 85.9% | (many diffs) |
| 6 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502085.3 | 82% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1104
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcaa gccgtcgaca atctcacctc tgcgcctggg aacaccagtc 121 tgtgcaccag agactacaaa atcacccagg tcctcttccc actgctctac actgtcctgt 181 tttttgttgg acttatcaca aatggcctgg cgatgaggat tttctttcaa atccggagta 241 aatcaaactt tattattttt cttaagaaca cagtcatttc tgatcttctc atgattctga 301 cttttccatt caaaattctt agtgatgcca aactgggaac aggaccactg agaacttttg 361 tgtgtcaagt tacctccgtc atattttatt tcacaatgta tatcagtatt tcattcctgg 421 gactgataac tatcgatcgc taccagaaga ccaccaggcc atttaaaaca tccaacccca 481 aaaatctctt gggggctaag attctctctg ttgtcatctg ggcattcatg ttcttactct 541 ctttgcctaa catgattctg accaacaggc agccgagaga caagaatgtg aagaaatgct 601 ctttccttaa atcagagttc ggtctagtct ggcatgaaat agtaaattac atctgtcaag 661 tcattttctg gattaatttc ttaattgtta ttgtatgtta tacactcatt acaaaagaac 721 tgtaccggtc atacgtaaga acgaggggtg taggtaaagt ccccaggaaa aaggtgaacg 781 tcaaagtttt cattatcatt gctgtattct ttatttgttt tgttcctttc cattttgccc 841 gaattcctta cacccTGAGC CAAACCCGGG ATGTCTTTGA CTGCACTGCT GAAAATACTC 901 TGTTCTATGT GAAAGAGAGC ACTCTGTGGT TAACTTCCTT AAATGCATGC CTGGATCCGT 961 TCATCTATTT TTTCCTTTGC AAGTCCTTCA GAAATTCCTT GATAAGTATG CTGAAGTGCC 1021 CCAATTCTGC AACATCTCTG TCCCAGGACA ATAGGAAAAA AGAACAGGAT GGTGGTGACC 1081 CAAATGAAGA GACTCCAATG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTAC TTCCTAGACC 1261 AAGAACGTAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt