Construct: ORF TRCN0000487738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020711.1_s317c1
- DNA Barcode:
- AAGGCGTCTGTAGCGGGATATGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PSKH2 (85481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85481 | PSKH2 | protein serine kinase H2 | NM_033126.3 | 100% | 100% | |
| 2 | human | 85481 | PSKH2 | protein serine kinase H2 | XM_017013929.1 | 76.3% | 68.6% | 1_286del;470_540del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1227
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggtgc ggcgccagca ggaaggtggt cccggggcca ccagcgctgg 121 cttgggccaa gcacgaaggt caaaaccaag ccggcgtcgg aggcgcgggg cctgggcccg 181 aggcggcggc ccaggcggcg cagaggatac aggtggctcg cttccgagcc aagttcgacc 241 cccgggtcct tgccagatat gacatcaaag ctcttattgg gacaggcagt ttcagcaggg 301 ttgtcagggt agagcagaag accaccaaga aaccttttgc aataaaagtg atggaaacca 361 gagagaggga aggtagagaa gcgtgcgtgt ctgagctgag cgtcctgcgg cgggttagcc 421 atcgttacat tgtccagctc atggagatct ttgagactga ggatcaagtt tacatggtaa 481 tggagctggc taccggaggg gagctctttg atcgactcat tgctcaggga tcctttacag 541 agcgggatgc cgtcaggatc ctccagatgg ttgctgatgg gattaggtat ttgcatgcgc 601 tgcagataac tcataggaat ctaaagcctg aaaacctctt atactatcat ccaggtgaag 661 agtcgaaaat tttaattaca gattttggtt tggcatactc cgggaaaaaa agtggtgact 721 ggacaatgaa gacactctgt gggaccccag agtacatagc tcctgaggtt ttgctaagga 781 agccttatac cagtgcagtg gacatgtggg ctcttggtgt gatcacatat gctttactta 841 gcggattcct gccttttgat gatgaaagcc agacaaggct ttacaggaag attctgaaag 901 gcaaatataa ttatacagga gagccttggc caagcatttc ccacttggcg aaggacttta 961 tagacaaact actgattttg gaggctggtc atcgcatgtc agctggccag gccctggacc 1021 atCCCTGGGT GATCACCATG GCTGCAGGGT CTTCCATGAA GAATCTCCAG AGGGCCATAT 1081 CCCGAAACCT CATGCAGAGG GCCTCTCCCC ACTCTCAGAG TCCTGGATCT GCACAGTCTT 1141 CTAAGTCACA TTATTCTCAC AAATCCAGGC ATATGTGGAG CAAGAGAAAC TTAAGGATAG 1201 TAGAATCGCC ACTGTCTGCG CTTTTGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAGGC 1381 GTCTGTAGCG GGATATGTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt