Construct: ORF TRCN0000487742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020623.1_s317c1
- DNA Barcode:
- ACACCCGCTTGAGCTACACACACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRB (5159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_002609.4 | 99.9% | 99.9% | 539C>T |
| 2 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355016.2 | 94.1% | 94.1% | 0_1ins192;347C>T |
| 3 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355017.2 | 83.5% | 81.8% | 0_1ins517;22C>T;114_147del |
| 4 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_008809.2 | 83.1% | 85.4% | (many diffs) |
| 5 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_001146268.1 | 83% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3390
- ORF length:
- 3318
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcggctt ccgggtgcga tgccagctct ggccctcaaa ggcgagctgc 121 tgttgctgtc tctcctgtta cttctggaac cacagatctc tcagggcctg gtcgtcacac 181 ccccggggcc agagcttgtc ctcaatgtct ccagcacctt cgttctgacc tgctcgggtt 241 cagctccggt ggtgtgggaa cggatgtccc aggagccccc acaggaaatg gccaaggccc 301 aggatggcac cttctccagc gtgctcacac tgaccaacct cactgggcta gacacgggag 361 aatacttttg cacccacaat gactcccgtg gactggagac cgatgagcgg aaacggctct 421 acatctttgt gccagatccc accgtgggct tcctccctaa tgatgccgag gaactattca 481 tctttctcac ggaaataact gagatcacca ttccatgccg agtaacagac ccacagctgg 541 tggtgacact gcacgagaag aaaggggacg ttgcactgcc tgtcccctat gatcaccaac 601 gtggcttttt tggtatcttt gaggacagaa gctacatctg caaaaccacc attggggaca 661 gggaggtgga ttctgatgcc tactatgtct acagactcca ggtgtcatcc atcaacgtct 721 ctgtgaacgc agtgcagact gtggtccgcc agggtgagaa catcaccctc atgtgcattg 781 tgatcgggaa tgaggtggtc aacttcgagt ggacataccc ccgcaaagaa agtgggcggc 841 tggtggagcc ggtgactgac ttcctcttgg atatgcctta ccacatccgc tccatcctgc 901 acatccccag tgccgagtta gaagactcgg ggacctacac ctgcaatgtg acggagagtg 961 tgaatgacca tcaggatgaa aaggccatca acatcaccgt ggttgagagc ggctacgtgc 1021 ggctcctggg agaggtgggc acactacaat ttgctgagct gcatcggagc cggacactgc 1081 aggtagtgtt cgaggcctac ccaccgccca ctgtcctgtg gttcaaagac aaccgcaccc 1141 tgggcgactc cagcgctggc gaaatcgccc tgtccacgcg caacgtgtcg gagacccggt 1201 atgtgtcaga gctgacactg gttcgcgtga aggtggcaga ggctggccac tacaccatgc 1261 gggccttcca tgaggatgct gaggtccagc tctccttcca gctacagatc aatgtccctg 1321 tccgagtgct ggagctaagt gagagccacc ctgacagtgg ggaacagaca gtccgctgtc 1381 gtggccgggg catgccccag ccgaacatca tctggtctgc ctgcagagac ctcaaaaggt 1441 gtccacgtga gctgccgccc acgctgctgg ggaacagttc cgaagaggag agccagctgg 1501 agactaacgt gacgtactgg gaggaggagc aggagtttga ggtggtgagc acactgcgtc 1561 tgcagcacgt ggatcggcca ctgtcggtgc gctgcacgct gcgcaacgct gtgggccagg 1621 acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg gtgatctcag 1681 ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc atgctttggc 1741 agaagaagcc acgttacgag atccgatgga aggtgattga gtctgtgagc tctgacggcc 1801 atgagtacat ctacgtggac cccatgcagc tgccctatga ctccacgtgg gagctgccgc 1861 gggaccagct tgtgctggga cgcaccctcg gctctggggc ctttgggcag gtggtggagg 1921 ccacggctca tggcctgagc cattctcagg ccacgatgaa agtggccgtc aagatgctta 1981 aatccacagc ccgcagcagt gagaagcaag cccttatgtc ggagctgaag atcatgagtc 2041 accttgggcc ccacctgaac gtggtcaacc tgttgggggc ctgcaccaaa ggaggaccca 2101 tctatatcat cactgagtac tgccgctacg gagacctggt ggactacctg caccgcaaca 2161 aacacacctt cctgcagcac cactccgaca agcgccgccc gcccagcgcg gagctctaca 2221 gcaatgctct gcccgttggg ctccccctgc ccagccatgt gtccttgacc ggggagagcg 2281 acggtggcta catggacatg agcaaggacg agtcggtgga ctatgtgccc atgctggaca 2341 tgaaaggaga cgtcaaatat gcagacatcg agtcctccaa ctacatggcc ccttacgata 2401 actacgttcc ctctgcccct gagaggacct gccgagcaac tttgatcaac gagtctccag 2461 tgctaagcta catggacctc gtgggcttca gctaccaggt ggccaatggc atggagtttc 2521 tggcctccaa gaactgcgtc cacagagacc tggcggctag gaacgtgctc atctgtgaag 2581 gcaagctggt caagatctgt gactttggcc tggctcgaga catcatgcgg gactcgaatt 2641 acatctccaa aggcagcacc tttttgcctt taaagtggat ggctccggag agcatcttca 2701 acagcctcta caccaccctg agcgacgtgt ggtccttcgg gatcctgctc tgggagatct 2761 tcaccttggg tggcacccct tacccagagc tgcccatgaa cgagcagttc tacaatgcca 2821 tcaaacgggg ttaccgcatg gcccagcctg cccatgcctc cgacgagatc tatgagatca 2881 tgcagaagtg ctgggaagag aagtttgaga ttcggccccc cttctcccag ctggtgctgc 2941 ttctcgagag actgttgggc gaaggttaca aaaagaagta ccagcaggtg gatgaggagt 3001 ttctgaggag tgaccaccca gccatccttc ggtcccaggc ccgcttgcct gggttccatg 3061 gcctccgatc tcccctggac accagctccg tcctctatac tgccgtgcag cccaatgagg 3121 gtgacaacga ctatatcatc cccctgcctg accccaaacc cgaggttgct gacgagggcc 3181 cactggaggg ttcccccagc ctagccagct ccaccctgaa tgaagtcaac acctcctcaa 3241 ccatcTCCTG TGACAGCCCC CTGGAGCCCC AGGACGAACC AGAGCCAGAG CCCCAGCTTG 3301 AGCTCCAGGT GGAGCCGGAG CCAGAGCTGG AACAGTTGCC GGATTCGGGG TGCCCTGCGC 3361 CTCGGGCGGA AGCAGAGGAT AGCTTCCTGT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 3421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 3541 ACCCGCTTGA GCTACACACA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 3601 ttgtgaaaga tt