Construct: ORF TRCN0000487743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020502.1_s317c1
- DNA Barcode:
- TTATCTCACTGTGTCGAGGCGGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTK7 (5754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_002821.5 | 100% | 100% | |
| 2 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_001270398.1 | 96.9% | 96.5% | (many diffs) |
| 3 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152880.4 | 96.2% | 96.2% | 1497_1498ins120 |
| 4 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152882.4 | 94.7% | 94.7% | 1878_1879ins168 |
| 5 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XM_011514765.2 | 93.2% | 92.9% | (many diffs) |
| 6 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152881.4 | 87.8% | 87.7% | 1228_1229ins390 |
| 7 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XM_011514766.2 | 85% | 84.5% | (many diffs) |
| 8 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NR_072997.2 | 71.3% | (many diffs) | |
| 9 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NR_072998.2 | 70.5% | 1_175del;2581_2582ins233;3153_3989del | |
| 10 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XR_926286.2 | 70.3% | (many diffs) | |
| 11 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | NM_175168.4 | 87.5% | 91.8% | (many diffs) |
| 12 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524922.2 | 86.6% | 90.2% | (many diffs) |
| 13 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524924.2 | 86.2% | 90.7% | (many diffs) |
| 14 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524923.1 | 85.7% | 89.3% | (many diffs) |
| 15 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_017317638.1 | 68.2% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3282
- ORF length:
- 3210
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggagct gcgcggggat ccccggccag accccgccgg ttgcctctgc 121 tcagcgtcct gctgctgccg ctgctgggcg gtacccagac agccattgtc ttcatcaagc 181 agccgtcctc ccaggatgca ctgcaggggc gccgggcgct gcttcgctgt gaggttgagg 241 ctccgggccc ggtacatgtg tactggctgc tcgatggggc ccctgtccag gacacggagc 301 ggcgtttcgc ccagggcagc agcctgagct ttgcagctgt ggaccggctg caggactctg 361 gcaccttcca gtgtgtggct cgggatgatg tcactggaga agaagcccgc agtgccaacg 421 cctccttcaa catcaaatgg attgaggcag gtcctgtggt cctgaagcat ccagcctcgg 481 aagctgagat ccagccacag acccaggtca cacttcgttg ccacattgat gggcaccctc 541 ggcccaccta ccaatggttc cgagatggga cccccctttc tgatggtcag agcaaccaca 601 cagtcagcag caaggagcgg aacctgacgc tccggccagc tggtcctgag catagtgggc 661 tgtattcctg ctgcgcccac agtgcttttg gccaggcttg cagcagccag aacttcacct 721 tgagcattgc tgatgaaagc tttgccaggg tggtgctggc accccaggac gtggtagtag 781 cgaggtatga ggaggccatg ttccattgcc agttctcagc ccagccaccc ccgagcctgc 841 agtggctctt tgaggatgag actcccatca ctaaccgcag tcgcccccca cacctccgca 901 gagccacagt gtttgccaac gggtctctgc tgctgaccca ggtccggcca cgcaatgcag 961 ggatctaccg ctgcattggc caggggcaga ggggcccacc catcatcctg gaagccacac 1021 ttcacctagc agagattgaa gacatgccgc tatttgagcc acgggtgttt acagctggca 1081 gcgaggagcg tgtgacctgc cttcccccca agggtctgcc agagcccagc gtgtggtggg 1141 agcacgcggg agtccggctg cccacccatg gcagggtcta ccagaagggc cacgagctgg 1201 tgttggccaa tattgctgaa agtgatgctg gtgtctacac ctgccacgcg gccaacctgg 1261 ctggtcagcg gagacaggat gtcaacatca ctgtggccac tgtgccctcc tggctgaaga 1321 agccccaaga cagccagctg gaggagggca aacccggcta cttggattgc ctgacccagg 1381 ccacaccaaa acctacagtt gtctggtaca gaaaccagat gctcatctca gaggactcac 1441 ggttcgaggt cttcaagaat gggaccttgc gcatcaacag cgtggaggtg tatgatggga 1501 catggtaccg ttgtatgagc agcaccccag ccggcagcat cgaggcgcaa gcccgtgtcc 1561 aagtgctgga aaagctcaag ttcacaccac caccccagcc acagcagtgc atggagtttg 1621 acaaggaggc cacggtgccc tgttcagcca caggccgaga gaagcccact attaagtggg 1681 aacgggcaga tgggagcagc ctcccagagt gggtgacaga caacgctggg accctgcatt 1741 ttgcccgggt gactcgagat gacgctggca actacacttg cattgcctcc aacgggccgc 1801 agggccagat tcgtgcccat gtccagctca ctgtggcagt ttttatcacc ttcaaagtgg 1861 aaccagagcg tacgactgtg taccagggcc acacagccct actgcagtgc gaggcccagg 1921 gggaccccaa gccgctgatt cagtggaaag gcaaggaccg catcctggac cccaccaagc 1981 tgggacccag gatgcacatc ttccagaatg gctccctggt gatccatgac gtggcccctg 2041 aggactcagg ccgctacacc tgcattgcag gcaacagctg caacatcaag cacacggagg 2101 cccccctcta tgtcgtggac aagcctgtgc cggaggagtc ggagggccct ggcagccctc 2161 ccccctacaa gatgatccag accattgggt tgtcggtggg tgccgctgtg gcctacatca 2221 ttgccgtgct gggcctcatg ttctactgca agaagcgctg caaagccaag cggctgcaga 2281 agcagcccga gggcgaggag ccagagatgg aatgcctcaa cggtgggcct ttgcagaacg 2341 ggcagccctc agcagagatc caagaagaag tggccttgac cagcttgggc tccggccccg 2401 cggccaccaa caaacgccac agcacaagtg ataagatgca cttcccacgg tctagcctgc 2461 agcccatcac cacgctgggg aagagtgagt ttggggaggt gttcctggca aaggctcagg 2521 gcttggagga gggagtggca gagaccctgg tacttgtgaa gagcctgcag agcaaggatg 2581 agcagcagca gctggacttc cggagggagt tggagatgtt tgggaagctg aaccacgcca 2641 acgtggtgcg gctcctgggg ctgtgccggg aggctgagcc ccactacatg gtgctggaat 2701 atgtggatct gggagacctc aagcagttcc tgaggatttc caagagcaag gatgaaaaat 2761 tgaagtcaca gcccctcagc accaagcaga aggtggccct atgcacccag gtagccctgg 2821 gcatggagca cctgtccaac aaccgctttg tgcataagga cttggctgcg cgtaactgcc 2881 tggtcagtgc ccagagacaa gtgaaggtgt ctgccctggg cctcagcaag gatgtgtaca 2941 acagtgagta ctaccacttc cgccaggcct gggtgccgct gcgctggatg tcccccgagg 3001 ccatcctgga gggtgacttc tctaccaagt ctgatgtctg ggccTTCGGT GTGCTGATGT 3061 GGGAAGTGTT TACACATGGA GAGATGCCCC ATGGTGGGCA GGCAGATGAT GAAGTACTGG 3121 CAGATTTGCA GGCTGGGAAG GCTAGACTTC CTCAGCCCGA GGGCTGCCCT TCCAAACTCT 3181 ATCGGCTGAT GCAGCGCTGC TGGGCCCTCA GCCCCAAGGA CCGGCCCTCC TTCAGTGAGA 3241 TTGCCAGCGC CCTGGGAGAC AGCACCGTGG ACAGCAAGCC GTAGGACCCA GCTTTCTTGT 3301 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3361 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3421 GAAAGGACGA TTATCTCACT GTGTCGAGGC GGCTACGCGT TAAGTCgaca atcaacctct 3481 ggattacaaa atttgtgaaa gatt