Construct: ORF TRCN0000487750

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020492.1_s317c1
DNA Barcode:
GACAGTGGCTCCGCGAAATTAGCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
STK38L (23012)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487750
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23012 STK38L serine/threonine kinase 38 ... NM_015000.4 99.9% 100% 993G>A
2 human 23012 STK38L serine/threonine kinase 38 ... XM_006719058.4 99.9% 100% 993G>A
3 human 23012 STK38L serine/threonine kinase 38 ... XM_024448894.1 99.9% 100% 993G>A
4 human 23012 STK38L serine/threonine kinase 38 ... XM_024448895.1 99.9% 100% 993G>A
5 human 23012 STK38L serine/threonine kinase 38 ... XM_024448896.1 99.9% 100% 993G>A
6 human 23012 STK38L serine/threonine kinase 38 ... XM_024448897.1 99.9% 100% 993G>A
7 human 23012 STK38L serine/threonine kinase 38 ... XM_005253342.4 91% 91.1% 182_183ins123;870G>A
8 human 23012 STK38L serine/threonine kinase 38 ... XM_024448889.1 88.9% 88.8% 776_946del;1164G>A
9 human 23012 STK38L serine/threonine kinase 38 ... XM_024448890.1 88.9% 88.8% 776_946del;1164G>A
10 human 23012 STK38L serine/threonine kinase 38 ... XM_024448891.1 88.9% 88.8% 776_946del;1164G>A
11 human 23012 STK38L serine/threonine kinase 38 ... XM_024448892.1 88.9% 88.8% 776_946del;1164G>A
12 human 23012 STK38L serine/threonine kinase 38 ... XM_024448893.1 88.9% 88.8% 776_946del;1164G>A
13 human 23012 STK38L serine/threonine kinase 38 ... XM_006719059.3 88.7% 88.7% 0_1ins156;837G>A
14 human 23012 STK38L serine/threonine kinase 38 ... XM_011520613.2 79.8% 79.9% 0_1ins156;26_27ins123;714G>A
15 human 23012 STK38L serine/threonine kinase 38 ... XR_001748626.1 27.1% (many diffs)
16 mouse 232533 Stk38l serine/threonine kinase 38 ... NM_172734.3 89.8% 97.6% (many diffs)
17 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_006507027.2 88.4% 96.1% (many diffs)
18 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_006507029.3 87.3% 94.9% (many diffs)
19 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_006507031.2 78% 85.3% (many diffs)
20 mouse 232533 Stk38l serine/threonine kinase 38 ... NM_001346666.1 66.6% 72.6% (many diffs)
21 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_017321560.1 66.6% 72.6% (many diffs)
22 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_006507032.2 65.6% 71.5% (many diffs)
23 mouse 232533 Stk38l serine/threonine kinase 38 ... XM_006507033.2 65.6% 71.5% (many diffs)
24 mouse 232533 Stk38l serine/threonine kinase 38 ... XR_001785124.1 18.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1464
ORF length:
1392
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcaatg acggcaggga ctacaacaac ctttcctatg agcaaccata
121cccgggaaag agtgactgta gccaagctca cattggagaa tttttatagc aacctaattt
181tacagcatga agagagagaa accaggcaga agaaattaga agtggccatg gaagaagaag
241gattagcaga tgaagagaaa aagttacgtc gatcacaaca cgctcgcaaa gaaacagagt
301tcttacggct caaaaggacc agacttggct tggatgactt tgagtctctg aaagttatag
361gaagaggagc ttttggagag gtgcggttgg tccagaagaa agatacaggc catatctatg
421caatgaagat attgagaaag tctgatatgc ttgaaaaaga gcaggtggcc catatccgag
481cagaaagaga tattttggta gaagcagatg gtgcctgggt ggtgaagatg ttttacagtt
541ttcaggataa gaggaatctt tatctaatca tggaatttct ccctggaggt gacatgatga
601cattgctaat gaagaaagac accttgacag aagaggaaac acagttctac atttcagaga
661ctgttctggc aatagatgcg atccaccagt tgggtttcat ccatcgggat attaagccag
721acaacctttt attggatgcc aagggtcatg taaaattatc tgattttggt ttatgtacgg
781gattaaagaa agctcacagg actgaatttt atagaaatct cacacacaac ccaccaagtg
841acttctcatt tcagaacatg aactcaaaga ggaaagcaga aacttggaag aagaacagga
901gacaactggc atattccaca gttgggacac cagattacat tgctccagaa gtattcatgc
961agactggtta caacaaattg tgtgactggt ggtctttggg agtgattatg tatgaaatgc
1021taataggata tccacctttc tgctctgaaa cacctcaaga aacatacaga aaagtgatga
1081actggaaaga aactctggta tttcctccag aggtacctat atctgagaaa gccaaggact
1141taattctcag attttgtatt gattctgaaa acagaattgg aaatagtgga gtagaagaaa
1201taaaaggtca tccctttttt gaaggtgtcg actgggagca cataagggaa aggccagcag
1261caaTCCCTAT AGAAATCAAA AGCATTGATG ATACTTCAAA TTTTGATGAC TTCCCTGAAT
1321CTGATATTTT ACAACCAGTG CCAAATACCA CAGAACCGGA CTACAAATCC AAAGACTGGG
1381TTTTTCTCAA TTATACCTAT AAAAGGTTTG AAGGGTTGAC TCAACGTGGC TCTATCCCCA
1441CCTACATGAA AGCTGGGAAG TTATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1501GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1561AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACAGTGG
1621CTCCGCGAAA TTAGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1681aagatt

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