Construct: ORF TRCN0000487759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019149.1_s317c1
- DNA Barcode:
- TCAAAGTAATATCTTCCCCTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMBR (4829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4829 | NMBR | neuromedin B receptor | NM_002511.3 | 99.7% | 99.4% | 678T>C;698A>G;1170_1171insG |
| 2 | human | 4829 | NMBR | neuromedin B receptor | NM_001324307.2 | 61.8% | 61.6% | (many diffs) |
| 3 | human | 4829 | NMBR | neuromedin B receptor | NM_001324308.2 | 61.8% | 61.6% | (many diffs) |
| 4 | mouse | 18101 | Nmbr | neuromedin B receptor | NM_008703.2 | 86.2% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1248
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgccc tctaagtctc tttccaacct ctcggtgacc accggcgcga 121 atgagagcgg ttccgttccc gaggggtggg aaagggattt cctgccggcc tcggacggga 181 ccaccacgga gttggtgatc cgctgtgtga tcccgtccct ctacctgctc atcatcaccg 241 tgggcttgct gggcaacatc atgctggtga agatcttcat caccaacagc gccatgagga 301 gcgtccccaa catcttcatc tctaacctgg cggccgggga cttgctgctg ctgctcacct 361 gcgtcccggt ggacgcctcg cgctacttct tcgacgagtg gatgtttggc aaggtgggct 421 gcaaactgat ccctgtcatc cagctcactt ccgtgggggt ttccgtgttc actctcactg 481 ccctcagcgc cgacaggtac agagccatcg ttaaccccat ggacatgcag acgtcagggg 541 cattgctgcg gacctgtgtg aaggccatgg gtatctgggt ggtctccgtg ttgctggcag 601 ttcccgaagc ggtgttttca gaagtggctc gcatcagtag cttggataat agcagcttca 661 cagcatgtat cccataccct caaacagatg aattacatcc aaagattcat tcagtgctca 721 ttttcttggt ctatttcctc ataccacttg ccattattag catttattat tgtcatattg 781 caaagacctt aattaaaagc gcacacaatc ttcctggaga atacaatgaa cataccaaaa 841 aacagatgga aacacggaaa cgcctggcta aaattgtgct tgtctttgtg ggctgtttca 901 tcttctgttg gtttccaaac cacatccttt acatgtatcg gtctttcaac tataatgaga 961 ttgatccatc tctaggccac atgattgtca ccTTAGTTGC CCGGGTTCTC AGTTTTGGCA 1021 ATTCTTGTGT CAACCCATTT GCTCTTTACC TACTCAGTGA AAGCTTCAGG AGGCATTTCA 1081 ACAGCCAACT CTGCTGTGGG AGGAAGTCCT ATCAAGAGAG AGGAACCAGC TACCTACTCA 1141 GCTCTTCAGC GGTGCGTATG ACATCTCTGA AAAGCAATGC TAAGAACATG GTGACCAATT 1201 CTGTTTTACT AAATGGGCAC AGCATGAAGC AGGAAATGGC ACTGGACCCA GCTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA TCAAAGTAAT ATCTTCCCCT CGTCACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt