Construct: ORF TRCN0000487764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020656.1_s317c1
- DNA Barcode:
- AGTAATCCAAATGCTTTATAAAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HUNK (30811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30811 | HUNK | hormonally up-regulated Neu... | NM_014586.2 | 99.9% | 100% | 1413T>C;1548T>C |
| 2 | human | 30811 | HUNK | hormonally up-regulated Neu... | XM_011529537.2 | 97.6% | 97.7% | 1255_1256ins48;1365T>C;1500T>C |
| 3 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | NM_015755.2 | 87.3% | 92.4% | (many diffs) |
| 4 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | XM_011246130.2 | 78.4% | 80.1% | (many diffs) |
| 5 | mouse | 26559 | Hunk | hormonally upregulated Neu-... | XR_384941.2 | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 2241
- ORF length:
- 2142
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacctcta gaagcgcgat gccggcggcg gcgggggacg 121 ggctcctggg ggagccggcg gcgcctgggg gcggcggcgg cgcggaggac gcggccaggc 181 ccgcggcggc ctgcgaggga agtttcctgc ctgcctgggt gagcggcgtg ccccgcgagc 241 ggctccgcga cttccagcac cacaagcgcg tgggcaacta cctcatcggc agcaggaagc 301 tgggcgaggg ctcctttgcc aaggtgcgcg aggggctgca cgtgctgacc ggggagaagg 361 tggccataaa agtcattgat aagaagagag ccaaaaagga cacctatgtc accaaaaacc 421 tgcggcgaga gggtcagatc cagcagatga tccgccaccc caatatcact cagctccttg 481 atattttaga aacggaaaac agctactacc tggtcatgga gctgtgccct gggggcaacc 541 tgatgcacaa gatctatgag aagaagcggc tggaggagtc cgaagcccgc agatacatcc 601 gacagctcat ctctgccgta gagcacctgc accgggccgg ggtggtccac agagacttga 661 agatagagaa tttgctacta gatgaagaca ataatatcaa gctgattgac tttggtttga 721 gcaactgcgc agggatcctg ggttactcgg atccgttcag cacacagtgt ggcagccctg 781 cctacgctgc acctgaactg ctcgccagga agaaatacgg ccccaaaatc gatgtctggt 841 ccataggtgt gaacatgtat gccatgttga ccgggacgct gcctttcacg gtggagcctt 901 tcagcctgag ggctttgtac cagaagatgg tagacaaaga aatgaacccc ctccccactc 961 agctctccac aggtgccatc agtttcctgc gctctctcct ggaaccggat cctgtgaaga 1021 ggccaaatat tcagcaggca ctggcgaatc gctggcttaa tgagaattac acgggcaaag 1081 tgccctgtaa tgtcacctat cccaacagga tttctctgga agatctgagc ccgagcgtcg 1141 tgctgcacat gaccgagaag ctgggttaca agaacagcga cgtgatcaac actgtgctct 1201 ccaaccgcgc ctgccacatc ctggccatct acttcctctt aaacaagaaa ctggagcgct 1261 atttgtcagg gaaatctgac atccaggaca gcctctgcta caagacccgg ctctaccaga 1321 tagaaaagta cagggccccc aaggagtcct atgaggcctc tctggacacc tggacacgag 1381 atcttgaatt ccatgccgtg caggataaaa agcccaaaga acaagaaaaa agaggggatt 1441 ttcttcatcg accattctcc aagaagttgg acaagaacct gccctcgcac aaacagccct 1501 caggctcgct catgacacag attcagaaca ccaaagccct cctgaaggac cggaaggcct 1561 ccaagtccag cttccccgac aaagattcct ttggctgccg caatattttc cgcaaaacct 1621 cagattccaa ttgtgtggct tcttcctcca tggagttcat ccccgtgcca ccgcccagga 1681 ccccgaggat tgtgaagaaa ccggagcccc atcagccagg gcccggaagc actggcatcc 1741 cccacaagga agaccccctg atgctggaca tggtgcgctc cttcgagtct gtggatcgcg 1801 acgaccacgt agaagtgctg tctccctctc atcactacag gattctgaac tccccggtca 1861 gcttggctcg cagaaattcc agcgagagga cgctgtcccc gggtctgcca tccggaagca 1921 tgtcgcctct ccatactcct ttgcatccaa ctctggtctc ttttgctcac gaagataaga 1981 acagcccccc aaaagaggag ggcctgtgtt gcccaccTCC GGTTCCCAGC AATGGCCCCA 2041 TGCAGCCTCT GGGGAGCCCC AATTGTGTGA AAAGCCGAGG CCGGTTCCCT ATGATGGGCA 2101 TCGGACAGAT GTTAAGGAAG CGCCATCAGA GTCTGCAGCC ATCTGCAGAT AGGCCCCTGG 2161 AGGCCAGCCT GCCCCCACTG CAGCCCCTAG CCCCTGTGAA CCTTGCCTTT GACATGGCCG 2221 ATGGGGTCAA GACCCAGTGC TAAAAGCTTA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT 2281 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2341 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2401 GAAAGGACGA AGTAATCCAA ATGCTTTATA AAAGACGCGT TAAGTCgaca atcaacctct 2461 ggattacaaa atttgtgaaa gatt