Construct: ORF TRCN0000487788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020130.1_s317c1
- DNA Barcode:
- GCGCACTGCGGATGATCGCCATGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SPHK2 (56848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204159.3 | 100% | 100% | |
| 2 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_020126.5 | 100% | 100% | |
| 3 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_011527133.1 | 100% | 100% | |
| 4 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204160.3 | 94.4% | 94.4% | 0_1ins108 |
| 5 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_006723292.1 | 94.4% | 94.4% | 0_1ins108 |
| 6 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_011527134.1 | 94.4% | 94.4% | 0_1ins108 |
| 7 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204158.3 | 90.9% | 90.9% | 36_37ins177 |
| 8 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_017027008.1 | 89.3% | 89.8% | (many diffs) |
| 9 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001243876.1 | 68.5% | 68.5% | 0_1ins618 |
| 10 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_017027010.2 | 54.3% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2034
- ORF length:
- 1962
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaatgga caccttgaag cagaggagca gcaggaccag aggccagacc 121 aggagctgac cgggagctgg ggccacgggc ctaggagcac cctggtcagg gctaaggcca 181 tggccccgcc cccaccgcca ctggctgcca gcaccccgct cctccatggc gagtttggct 241 cctacccagc ccgaggccca cgctttgccc tcacccttac atcgcaggcc ctgcacatac 301 agcggctgcg ccccaaacct gaagccaggc cccggggtgg cctggtcccg ttggccgagg 361 tctcaggctg ctgcaccctg cgaagccgca gcccctcaga ctcagcggcc tacttctgca 421 tctacaccta ccctcggggc cggcgcgggg cccggcgcag agccactcgc accttccggg 481 cagatggggc cgccacctac gaagagaacc gtgccgaggc ccagcgctgg gccactgccc 541 tcacctgtct gctccgagga ctgccactgc ccggggatgg ggagatcacc cctgacctgc 601 tacctcggcc gccccggttg cttctattgg tcaatccctt tgggggtcgg ggcctggcct 661 ggcagtggtg taagaaccac gtgcttccca tgatctctga agctgggctg tccttcaacc 721 tcatccagac agaacgacag aaccacgccc gggagctggt ccaggggctg agcctgagtg 781 agtgggatgg catcgtcacg gtctcgggag acgggctgct ccatgaggtg ctgaacgggc 841 tcctagatcg ccctgactgg gaggaagctg tgaagatgcc tgtgggcatc ctcccctgcg 901 gctcgggcaa cgcgctggcc ggagcagtga accagcacgg gggatttgag ccagccctgg 961 gcctcgacct gttgctcaac tgctcactgt tgctgtgccg gggtggtggc cacccactgg 1021 acctgctctc cgtgacgctg gcctcgggct cccgctgttt ctccttcctg tctgtggcct 1081 ggggcttcgt gtcagatgtg gatatccaga gcgagcgctt cagggccttg ggcagtgccc 1141 gcttcacact gggcacggtg ctgggcctcg ccacactgca cacctaccgc ggacgcctct 1201 cctacctccc cgccactgtg gaacctgcct cgcccacccc tgcccatagc ctgcctcgtg 1261 ccaagtcgga gctgacccta accccagacc cagccccgcc catggcccac tcacccctgc 1321 atcgttctgt gtctgacctg cctcttcccc tgccccagcc tgccctggcc tctcctggct 1381 cgccagaacc cctgcccatc ctgtccctca acggtggggg cccagagctg gctggggact 1441 ggggtggggc tggggatgct ccgctgtccc cggacccact gctgtcttca cctcctggct 1501 ctcccaaggc agctctacac tcacccgtct ccgaaggggc ccccgtaatt cccccatcct 1561 ctgggctccc acttcccacc cctgatgccc gggtaggggc ctccacctgc ggcccgcccg 1621 accacctgct gcctccgctg ggcaccccgc tgcccccaga ctgggtgacg ctggaggggg 1681 actttgtgct catgttggcc atctcgccca gccacctagg cgctgacctg gtggcagctc 1741 cgcatgcgcg cttcgacgac ggcctggtgc acctgtgctg ggtgcgtagc ggcatctcgc 1801 gggctgcgct gctgcgccTT TTCTTGGCCA TGGAGCGTGG TAGCCACTTC AGCCTGGGCT 1861 GTCCGCAGCT GGGCTACGCC GCGGCCCGTG CCTTCCGCCT AGAGCCGCTC ACACCACGCG 1921 GCGTGCTCAC AGTGGACGGG GAGCAGGTGG AGTATGGGCC GCTACAGGCA CAGATGCACC 1981 CTGGCATCGG TACACTGCTC ACTGGGCCTC CTGGCTGCCC GGGGCGGGAG CCCTGAGACC 2041 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2101 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2161 ATATATCTTG TGGAAAGGAC GAGCGCACTG CGGATGATCG CCATGTACGC GTTAAGTCga 2221 caatcaacct ctggattaca aaatttgtga aagatt