Construct: ORF TRCN0000487797

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021457.1_s317c1
DNA Barcode:
CAACAAACAGTAACTTGAGACCAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
GNAL (2774)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487797
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2774 GNAL G protein subunit alpha L NM_182978.4 99.3% 100% 243G>T;1374_1375insTGAAAGCT
2 human 2774 GNAL G protein subunit alpha L NM_001142339.2 78.2% 75.5% (many diffs)
3 human 2774 GNAL G protein subunit alpha L NM_001261443.1 78.2% 75.5% (many diffs)
4 human 2774 GNAL G protein subunit alpha L NM_001369387.1 78.2% 75.5% (many diffs)
5 human 2774 GNAL G protein subunit alpha L XM_006722324.3 52% 52.4% 243G>T;720_721ins662
6 human 2774 GNAL G protein subunit alpha L NM_001261444.2 37.7% 37.9% 0_1ins852;522_523insTGAAAGCT
7 mouse 14680 Gnal guanine nucleotide binding ... NM_177137.5 84.8% 91% (many diffs)
8 mouse 14680 Gnal guanine nucleotide binding ... NM_010307.3 72.7% 75.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1440
ORF length:
1374
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat
61tcaccatggg tctgtgctac agtctgcggc cgctgctttt cgggggccca ggggacgacc
121cctgcgcggc ctcggagccg ccggtggagg acgcgcagcc cgccccggcc ccggccctgg
181ccccagtccg ggcggccgca agggacacgg cccggaccct gctccctcgg ggcggcgaag
241ggagcccggc atgcgctcgg cccaaagcag acaagccgaa ggagaagcgg cagcgcaccg
301agcagcttag tgccgaggag cgcgaggcgg ccaaggagcg cgaggcggtc aaggaggcga
361ggaaagtgag ccggggcatc gaccgcatgc tgcgcgacca gaagcgcgac ctgcagcaga
421cgcaccggct cctgctgctc ggggctggtg agtctgggaa aagcactatc gtcaaacaga
481tgaggatcct gcacgtcaat gggtttaatc ccgaggaaaa gaaacagaaa attctggaca
541tccggaaaaa tgttaaagat gctatcgtga caattgtttc agcaatgagt actataatac
601ctccagttcc gctggccaac cctgaaaacc aatttcgatc agactacatc aagagcatag
661cccctatcac tgactttgaa tattcccagg aattctttga ccatgtgaaa aaactttggg
721acgatgaagg cgtgaaggca tgctttgaga gatccaacga ataccagctg attgactgtg
781cacaatactt cctggaaaga atcgacagcg tcagcttggt tgactacaca cccacagacc
841aggacctcct cagatgcaga gttctgacat ctgggatttt tgagacacga ttccaagtgg
901acaaagtaaa cttccacatg tttgatgttg gtggccagag ggatgagagg agaaaatgga
961tccagtgctt taacgatgtc acagctatca tttacgtcgc agcctgcagt agctacaaca
1021tggtgattcg agaagataac aacaccaaca ggctgagaga gtccctggat ctttttgaaa
1081gcatctggaa caacaggtgg ttacggacca tttctatcat cttgttcttg aacaaacaag
1141atatgctggc agaaaaagtc ttGGCAGGGA AATCAAAAAT TGAAGACTAT TTCCCAGAAT
1201ATGCAAATTA TACTGTTCCT GAAGACGCAA CACCAGATGC AGGAGAAGAT CCCAAAGTTA
1261CAAGAGCCAA GTTCTTTATC CGGGACCTGT TTTTGAGGAT CAGCACGGCC ACCGGTGACG
1321GCAAACATTA CTGCTACCCG CACTTCACCT GCGCCGTGGA CACAGAGAAC ATCCGCAGGG
1381TGTTCAACGA CTGCCGCGAC ATCATCCAGC GGATGCACCT CAAGCAGTAT GAGCTCTTGT
1441GAAAGCTTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1501CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1561TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAACAA ACAGTAACTT GAGACCATAC
1621GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)