Construct: ORF TRCN0000487818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019195.1_s317c1
- DNA Barcode:
- ATCAGAGCCACGGTGCAACCACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRY2 (1408)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_021117.5 | 100% | 100% | |
| 2 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_001127457.2 | 89.3% | 88% | (many diffs) |
| 3 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | NM_009963.4 | 90.2% | 94.2% | (many diffs) |
| 4 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | XM_017315281.1 | 90.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1854
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcg gcgactgtgg cgacggcggc agctgtggcc ccggcgccag 121 cgcccggcac ggacagcgcc tcttcggtgc actggttccg caaagggctg cgactccacg 181 acaacccggc gttgctggcg gccgtgcgcg gggcgcgctg cgtgcgctgc gtttacattc 241 tcgacccgtg gttcgcggcc tcctcctcag tcgggatcaa ccgatggagg ttcctacttc 301 agtctctgga agatttggac acaagtttaa ggaaactgaa ctcccgcctg tttgtagtcc 361 ggggacagcc agccgacgtg ttcccaaggc tgttcaagga atggggagtg acccgcttga 421 cctttgaata tgactctgaa ccctttggga aagaacggga tgcagccatc atgaagatgg 481 ccaaggaggc tggtgtggaa gtagtgacgg agaattctca taccctctat gacctggaca 541 ggatcattga gctgaatggg cagaagccac cccttacata caagcgcttt caggccatca 601 tcagccgcat ggagctgccc aagaagccag tgggcttggt gaccagccag cagatggaga 661 gctgcagggc cgagatccag gagaaccacg acgagaccta cggcgtgccc tccctggagg 721 agctggggtt ccccactgaa ggacttggtc cagctgtctg gcagggagga gagacagaag 781 ctctggcccg cctggataag cacttggaac ggaaggcctg ggttgccaac tatgagagac 841 cccgaatgaa cgccaactcc ctcctggcca gccccacagg cctcagcccc tacctgcgct 901 ttggttgtct ctcctgccgc ctcttctact accgcctgtg ggacctgtat aaaaaggtga 961 agcggaacag cacacctccc ctctccctat ttgggcaact cctatggcga gagttcttct 1021 acacggcagc taccaacaac cccaggtttg accgcatgga ggggaacccc atctgcatcc 1081 agatcccctg ggaccgcaat cctgaggccc tggccaagtg ggctgagggc aagacaggct 1141 tcccttggat tgatgccatc atgacccaac tgaggcagga gggctggatc caccacctgg 1201 cccggcatgc cgtggcctgc ttcctgaccc gcggggacct ctgggtcagc tgggagagcg 1261 gggtccgggt atttgatgag ctgctcctgg atgcagattt cagcgtgaac gcaggcagct 1321 ggatgtggct gtcctgcagt gctttcttcc agcagttctt ccactgctac tgccctgtgg 1381 gctttggccg tcgcacggac cccagtgggg actacatcag gcgatacctg cccaaattga 1441 aagcgttccc ctctcgatac atctatgagc cctggaatgc cccagagtca attcagaagg 1501 cagccaagtg catcattggt gtggactacc cacggcccat cgtcaaccat gccgagacca 1561 gccggcttaa cattgaacga atgaagcaga tttaccagca gctttcgcgc taccggggac 1621 tctgtctact ggcatctgtc ccttcctgtg tggaagaccT CAGTCACCCT GTGGCAGAGC 1681 CCAGCTCGAG CCAGGCTGGC AGCATGAGCA GTGCAGGCCC AAGACCACTA CCCAGTGGCC 1741 CAGCATCCCC CAAACGCAAG CTGGAAGCAG CCGAGGAACC ACCTGGTGAA GAACTCAGCA 1801 AACGGGCCCG GGTGGCAGAG TTGCCAACCC CAGAGCTGCC GAGCAAGGAT GCCGACCCAG 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAA TCAGAGCCAC GGTGCAACCA CGTACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att