Construct: ORF TRCN0000487826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020595.1_s317c1
- DNA Barcode:
- CAGTTAACCTTGTCTCTGTAGCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHB2 (2048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487826
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2048 | EPHB2 | EPH receptor B2 | NM_017449.4 | 100% | 100% | |
| 2 | human | 2048 | EPHB2 | EPH receptor B2 | NM_004442.7 | 99.8% | 99.8% | 1699_1701delAGA |
| 3 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710441.4 | 98.8% | 97.2% | (many diffs) |
| 4 | human | 2048 | EPHB2 | EPH receptor B2 | XM_006710442.4 | 96.7% | 96.7% | 1426_1427ins93;1452T>C;1606_1608delAGA |
| 5 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309192.2 | 94% | 94% | 1545T>C;1591_1592ins174 |
| 6 | human | 2048 | EPHB2 | EPH receptor B2 | NM_001309193.2 | 93.3% | 93.3% | 1545T>C;2957_3165delinsTT |
| 7 | human | 2048 | EPHB2 | EPH receptor B2 | XM_024453895.1 | 55.2% | 55.2% | 0_1ins1323;222T>C |
| 8 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_010142.4 | 90.9% | 99.4% | (many diffs) |
| 9 | mouse | 13844 | Ephb2 | Eph receptor B2 | NM_001290753.2 | 90.8% | 99.3% | (many diffs) |
| 10 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538531.3 | 90.8% | 99.3% | (many diffs) |
| 11 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538534.2 | 90.7% | 99.2% | (many diffs) |
| 12 | mouse | 13844 | Ephb2 | Eph receptor B2 | XM_006538533.3 | 87.8% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3030
- ORF length:
- 2958
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctctg cggaggctgg gggccgcgct gctgctgctg ccgctgctcg 121 ccgccgtgga agaaacgcta atggactcca ctacagcgac tgctgagctg ggctggatgg 181 tgcatcctcc atcagggtgg gaagaggtga gtggctacga tgagaacatg aacacgatcc 241 gcacgtacca ggtgtgcaac gtgtttgagt caagccagaa caactggcta cggaccaagt 301 ttatccggcg ccgtggcgcc caccgcatcc acgtggagat gaagttttcg gtgcgtgact 361 gcagcagcat ccccagcgtg cctggctcct gcaaggagac cttcaacctc tattactatg 421 aggctgactt tgactcggcc accaagacct tccccaactg gatggagaat ccatgggtga 481 aggtggatac cattgcagcc gacgagagct tctcccaggt ggacctgggt ggccgcgtca 541 tgaaaatcaa caccgaggtg cggagcttcg gacctgtgtc ccgcagcggc ttctacctgg 601 ccttccagga ctatggcggc tgcatgtccc tcatcgccgt gcgtgtcttc taccgcaagt 661 gcccccgcat catccagaat ggcgccatct tccaggaaac cctgtcgggg gctgagagca 721 catcgctggt ggctgcccgg ggcagctgca tcgccaatgc ggaagaggtg gatgtaccca 781 tcaagctcta ctgtaacggg gacggcgagt ggctggtgcc catcgggcgc tgcatgtgca 841 aagcaggctt cgaggccgtt gagaatggca ccgtctgccg aggttgtcca tctgggactt 901 tcaaggccaa ccaaggggat gaggcctgta cccactgtcc catcaacagc cggaccactt 961 ctgaaggggc caccaactgt gtctgccgca atggctacta cagagcagac ctggaccccc 1021 tggacatgcc ctgcacaacc atcccctccg cgccccaggc tgtgatttcc agtgtcaatg 1081 agacctccct catgctggag tggacccctc cccgcgactc cggaggccga gaggacctcg 1141 tctacaacat catctgcaag agctgtggct cgggccgggg tgcctgcacc cgctgcgggg 1201 acaatgtaca gtacgcacca cgccagctag gcctgaccga gccacgcatt tacatcagtg 1261 acctgctggc ccacacccag tacaccttcg agatccaggc tgtgaacggc gttactgacc 1321 agagcccctt ctcgcctcag ttcgcctctg tgaacatcac caccaaccag gcagctccat 1381 cggcagtgtc catcatgcat caggtgagcc gcaccgtgga cagcattacc ctgtcgtggt 1441 cccagccgga ccagcccaat ggcgtgatcc tggactatga gctgcagtac tatgagaagg 1501 agctcagtga gtacaacgcc acagccataa aaagccccac caacacggtc accgtgcagg 1561 gcctcaaagc cggcgccatc tatgtcttcc aggtgcgggc acgcaccgtg gcaggctacg 1621 ggcgctacag cggcaagatg tacttccaga ccatgacaga agccgagtac cagacaagca 1681 tccaggagaa gttgccactc atcatcggct cctcggccgc tggcctggtc ttcctcattg 1741 ctgtggttgt catcgccatc gtgtgtaaca gacgggggtt tgagcgtgct gactcggagt 1801 acacggacaa gctgcaacac tacaccagtg gccacatgac cccaggcatg aagatctaca 1861 tcgatccttt cacctacgag gaccccaacg aggcagtgcg ggagtttgcc aaggaaattg 1921 acatctcctg tgtcaaaatt gagcaggtga tcggagcagg ggagtttggc gaggtctgca 1981 gtggccacct gaagctgcca ggcaagagag agatctttgt ggccatcaag acgctcaagt 2041 cgggctacac ggagaagcag cgccgggact tcctgagcga agcctccatc atgggccagt 2101 tcgaccatcc caacgtcatc cacctggagg gtgtcgtgac caagagcaca cctgtgatga 2161 tcatcaccga gttcatggag aatggctccc tggactcctt tctccggcaa aacgatgggc 2221 agttcacagt catccagctg gtgggcatgc ttcggggcat cgcagctggc atgaagtacc 2281 tggcagacat gaactatgtt caccgtgacc tggctgcccg caacatcctc gtcaacagca 2341 acctggtctg caaggtgtcg gactttgggc tctcacgctt tctagaggac gatacctcag 2401 accccaccta caccagtgcc ctgggcggaa agatccccat ccgctggaca gccccggaag 2461 ccatccagta ccggaagttc acctcggcca gtgatgtgtg gagctacggc attgtcatgt 2521 gggaggtgat gtcctatggg gagcggccct actgggacat gaccaaccag gatgtaatca 2581 atgccattga gcaggactat cggctgccac cgcccatgga ctgcccgagc gccctgcacc 2641 aactcatgct ggactgttgg cagaaggacc gcaaccaccg gcccaagttc ggccaaattg 2701 tcaacacgct agacaagatg atccgcaatc ccaacagcct caaagccatg gcgcccctct 2761 ccTCTGGCAT CAACCTGCCG CTGCTGGACC GCACGATCCC CGACTACACC AGCTTTAACA 2821 CGGTGGACGA GTGGCTGGAG GCCATCAAGA TGGGGCAGTA CAAGGAGAGC TTCGCCAATG 2881 CCGGCTTCAC CTCCTTTGAC GTCGTGTCTC AGATGATGAT GGAGGACATT CTCCGGGTTG 2941 GGGTCACTTT GGCTGGCCAC CAGAAAAAAA TCCTGAACAG TATCCAGGTG ATGCGGGCGC 3001 AGATGAACCA GATTCAGTCT GTGGAGGTTT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 3061 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3121 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 3181 GTTAACCTTG TCTCTGTAGC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 3241 ttgtgaaaga tt